Abstract
The abilities of various sorbents to adsorb catalase (CAT; EC 1.11.1.6) from filtered culture liquid (FCL) of the fungus Penicillium piceum F-648 were compared. Potassium phosphate, hydroxyapatite (HAP), and coprecipitated sorbents containing calcium phosphate and magnesium hydroxide adsorbed extracellular CAT more efficiently than aluminum oxide, aluminum phosphate, or quartz sand. The enzyme was isolated from FCL of Penicillium piceum with the use of HAP and a binary coprecipitated sorbent, Ca3(PO4)2 + Mg(OH)2, 1 : 1 (CM). The CAT(CM) sample contained the least amount of protein admixture. Its spectra had absorption maximums at 279.6, 406.8 (Soret band), 540, 585, 636, and 703 nm and negative molar ellipticity minimums at 207 and 210–214 nm. The kinetic indices of the samples (K M, V max : K M, and specific activity) were intricately dependent on the protein concentration in the reaction mixture. In dilute solutions, the K M and specific activities of CAT(CM) and CAT(HAP) equaled 667 and 137 mM; 300.9 × 104 and 30.0 × 104 U/mg protein, respectively. The effective velocity constants of inactivation of CAT(HAP), CAT(CM), and FCL in the reaction of H2O2 decomposition increased dramatically after the dilution of samples. In the infinitely dilute solution, they were 4.30 × 10–2, 6.46 × 10–2, and 1.12 × 10–2 s–1, respectively.
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REFERENCES
Miroshnichenko, O.S., Biopolimery i Kletka, 1992, vol. 8, no. 6, pp. 3–25.
Switala, J. and Loewen, P.C., Arch. Biochem. Biophys., 2002, vol. 401, no. 2, pp. 145–154.
Maksimenko, A.V., Uspekhi Sovrem. Biol., 1993, vol. 113, no. 2, pp. 351–365.
Mikhailova, R.V., Lobanok, A.G., Shishko, Zh.F., and Yasenko, M.I., Mikol. Fitopatol., 1998, vol. 32, no. 1, pp. 60–64.
Kurakov, A.V., Kupletskaya, M.B., Skrinnikova, E.V., and Somova, N.G., Prikl. Biokhim. Mikrobiol., 2001, vol. 37, no. 1, pp. 67–72.
Gulyi, M.F., Gudkova, L.V., Degtyar', R.G., Mironenko, N.I., and Latyshko, N.V., Dokl. Akad. Nauk SSSR, 1975, vol. 225, no. 1, pp. 211–213.
Kikuchi-Torii, K., Hayashi, S., Nakamoto, H., and Naka-mura, S., J. Biochem. (Tokyo), 1982, vol. 92, no. 5, pp. 1449–1456.
Gorbunoff, M.J., Anal. Biochem., 1984, vol. 136, no. 2, pp. 425–432.
Latyshko, N.V. and Gudkova, L.V., Ukr. Biokhim. Zh., 1996, vol. 68, no. 2, pp. 69–73.
Eremin, A.N., Mikhailova, R.V., and Metelitsa, D.I., Prikl. Biokhim. Mikrobiol., 2000, vol. 36, no. 3, pp. 261–266.
Eremin, A.N., Moroz, I.V., Pavlovskaya, Zh.I., and Mikhailova, R.V., Vestnik Fonda Fundamental'Nykh Issledovanii (Belarus'), 2002, no. 2, pp. 32–45.
Pavlovskaya, Zh.I., Mikhailova, R.V., Moroz, I.V., and Eremin, A.N., Prikl. Biokhim. Mikrobiol., 2003, vol. 39, no. 1, pp. 31–36.
Knopov, V.E. and Degtyar', R.G., Ukr. Biokhim. Zh., 1983, vol. 55, no. 5, pp. 494–498.
Drozhdenyuk, A.P., Eremin, A.N., Moroz, I.V., Pav-lovskaya, Zh.I., and Mikhailova, R.V., Vestsi NAN Belarusi, Ser.: Khim. Navuki, 2002, no. 2, pp. 76–82.
Komarov, V.S., Adsorbenty. Voprosy teorii, sinteza i struktury (Adsorbents: Problems of Theory, Synthesis, and Structure), Minsk: Belaruskaya navuka, 1997.
Orlovskii, V.P., Ezhova, Zh.A., Rodicheva, G.V., Koval', E.M., Sukhanova, G.E., and Tezikova, L.A., Zhurn. Neorg. Khim, 1992, vol. 37, no. 4, pp. 881–883.
Sedmak, J.J. and Grossberg, S.E., Anal. Biochem., 1977, vol. 79, nos. 1–2, pp. 544–552.
Eremin, A.N., Litvinchuk, A.V., and Metelitsa, D.I., Biokhimiya, 1996, vol. 61, no. 4, pp. 664–679.
Matheis, W., Zott, A., and Schwanig, M., Vaccine, 2000, vol. 20, no. 1, pp. 67–73.
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Eremin, A.N., Makarenko, M.V., Drozhdenyuk, A.P. et al. Precipitated, Coprecipitated, and Carbon–Mineral Sorbents for Isolation of Exracellular Catalase from Penicillium piceum F-648. Applied Biochemistry and Microbiology 40, 433–440 (2004). https://doi.org/10.1023/B:ABIM.0000040663.66205.d9
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DOI: https://doi.org/10.1023/B:ABIM.0000040663.66205.d9