Abstract
Oxidative stress has been associated in several inflammatory conditions and incriminated in the pathogenesis of many diseases. However, little information is available on the status of plasma antioxidant levels, essential components of important antioxidant enzymes such as copper, zinc and selenium in blood, and the end product of oxidative damage to the erythrocytic polyunsaturated fatty acids in inflammatory udder conditions. Blood samples were collected from three groups of dairy cows, with 21 in each group: animals with healthy udder, clinical mastitis, and subclinical mastitis. These animals were randomly selected from a herd on the basis of the California mastitis test, somatic cell count and total bacterial count. The mean plasma ascorbic acid concentration was significantly lower in cows with subclinical (p = 0.004) and clinical mastitis (p = 0.000) and the erythrocytic lipid peroxide levels were significantly (p = 0.000) higher in clinical mastitis as compared to controls. There was a significant decrease in mean blood zinc concentration in subclinical (p = 0.005) and clinical mastitis (p = 0.000), but an increase in mean blood copper level in the clinical mastitis group. It was concluded that the blood antioxidant status declines in inflammatory udder conditions, suggesting that incorporation of antioxidants may help in better management of mastitis in dairy cows.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Atroshi, F., Rizzo, A., Osterman, T. and Parantainene, J., 1989. Free fatty acids and lipid peroxidation in normal and mastitic bovine milk. Journal of Veterinary Medicine Series A, 36, 321–330
Bitman, J., Wood, D.L., Bright, S.A., Miller, R.H., Capuco, A.V., Roche, A. and Pankey, J.W., 1991. Lipid composition of teat canal keratin collected before and after milking from Holstein and Jersey cows. Journal of Dairy Science, 74, 414–420
Braun, U., Forrer, R., Furer, W. and Lutz, H., 1991. Seleniumand vitamin E in blood sera of cows from farms with increased incidence of disease. Veterinary Record, 128, 543–547
Burk, K. and Raymond, F., 2002. Selenium, an antioxidant nutrient. Nutrition and Clinical Care, 5, 75–79
Conner, J.G., Eckersall, P.D., Doherty, M. and Douglas, T.A., 1986. Acute phase response and mastitis in the cow. Research in Veterinary Science, 41,126–128
Cuzzocrea, S., Riley, D.P., Caputi, A.P. and Salvemini, D., 2001. Antioxidant therapy: a new pharmacological approach in shock, in£ammation, and ischemia/reperfusion injury. Pharmacological Reviews, 53, 135–159
Erskine, R.J. and Bartlett, P.C., 1993. Serumconcentrations of copper, iron and zinc during Escherichia coli induced mastitis. Journal of Dairy Science., 76,408–413
Erskine, R.J., Eberhart, R.J. and Scholz, R.W., 1988. Experimental E. coli mastitis in selenium-de¢cient and selenium-adequate cows. Journal of Dairy Science, 71s,150
Evans, P. and Halliwell, B., 2001. Micronutrients. Oxidant/antioxidant status. British Journal of Nutrition, 85,s57–s74
Gri¤n, T.K., Dodd, F.H., Neave, F.K., Westgarth, D.R., Kingwill, R.G. and Wilson, C.D., 1977. A method of diagnosing intramammary infection in dairy cows for large experiments. Journal of Dairy Research, 44, 25–45
Gupta, V.K., Kumar, A. and Sharma, S.D., 1999. Plasma ascorbic acid in healthy and subclinical mastitic goats. Indian Journal of Veterinary Medicine, 19, 83–85
Halliwell, B. and Gutteridge, J.M.C., 1999. Free Radicals in Biology and Medicine, 3rd edn, (Oxford University Press, Oxford)
Halliwell, B., Wasil, M. and Grootveld, M., 1987. Biologically signi¢cant scavenging of myeloperoxidasederived oxidant hypochlorous acid by ascorbic acid. FEBS Letters, 213,15–17
Jagota, S.K. and Dani, H.M., 1982. A new colorimetric technique for the estimation of vitamin C using Folin phenol reagent. Analytical Biochemistry, 127, 178–182
Kolb, E., 1992. Recent ¢ndings of the signi¢cance of ascorbic acid for domestic animals and its uses in veterinary medicine. Tierarztliche-Umschau, 47, 163–175
Kolmer, J.A., Spandling, E.H. and Robinson, H.W., 1951. Approved Laboratory Techniques, (Appleton Century Croft, New York), 1090–1091
Kossaibati, M.A. and Esslemont, R.J., 1997. The costs of production diseases in dairy herds in England. The Veterinary Journal, 154, 41–51
Lamand, M and Levieux, D., 1981. E¡ects of infection on plasma levels of copper and zinc in ewes. Annales de Recherches Veterinaires, 12,133–136
Mass, J.P., 1983. Diagnosis and management of selenium responsive diseases in cattle. Compendium of Continuing Education in Practicing Veterinary, 5, s393–s399
Nockels, C.F. and Blair, R., 1996. Antioxidants improve cattle immunity following stress. Animal Feed Science and Technology, 62,59–68
Patra, R.C. and Swarup, D., 2000. E¡ect of lead on erythrocytic antioxidant defense, lipid peroxide level and thiol groups in calves. Research in Veterinary Science, 68, 71–74
Placer, Z.A., Cushman, L. and Johnson, B., 1966. Estimation of product of lipid peroxidation (malonyldialdehyde) in biochemical systems. Analytical Biochemistry, 16, 359–364
Ram Naresh, Dwivedi, S.K., Dey, S. and Swarup, D., 2001. Zinc, copper and cobalt concentrations in blood during in£ammation of the mammary gland in dairy cows. Asian-Australian Journal of Animal Science, 14, 564–566
Roth, J.A. and Kaeberle, M.L., 1985. In vivo e¡ect of ascorbic acid on neutrophil function in healthy and dexamethasone treated cattle. American Journal of Veterinary Research, 46, 2434–2436
Schalm, O.W., Carrol, E.J. and Jain, N.C., 1971. Bovine Mastitis, (Lea and Febiger, Philadelphia), 95–156
Singh, K.B. and Bansal, B.K., 2001. Control of mastitis: a nutritional approach. In: Proceedings of the Round Table on Mastitis, VIII Annual Conference of Indian Association for Advancement of Veterinary Research, Bareilly (UP), India, 76–80
Snedecor, G.W. and Cochran, W.G., 1989. Statistical Methods, 8th edn, (Oxford and IBH, New Delhi)
Sorenson, J.R.S., 1987. Anti-in£ammatory, antiulcer and analgesic activities of copper complex o¡er a pharmacological approach to treatment of rheumatoid arthritis. In: Proceedings of the International Symposium on Current Trends in Trace Elements Research, Paris
Spain, J., 1993. Tissue integrity: a key defence against mastitis infection: the role of zinc proteinates and a theory for mode of action. In: Biotechnology in the Feed Industry, Proceedings of Alltech's 9th Annual Symposium, (Alltech Technical Publications, Nicholasville, KY) 53–60
Stevens, J.B., Olson, W.G., Kraemer, R. and Archambeau, J., 1985. Serumseleniumconcentrations and glutathione peroxidase activities in cattle grazing forages of various seleniumconcentrations. American Journal of Veterinary Research, 46, 1556–1560
Utley, H.G., Bernheim, F. and Hochseim, P., 1967. E¡ect of sulfahydryl reagents on peroxidation of microsomes. Archives of Biochemistry and Biophysics, 118, 29–32
Vankampen, E.J. and Ziglstra, W.G., 1961. Colorimetric determination of haemoglobin. Clinica Chimica Acta, 6, 3588
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ranjan, R., Swarup, D., Naresh, R. et al. Enhanced Erythrocytic Lipid Peroxides and Reduced Plasma Ascorbic Acid, and Alteration in Blood Trace Elements Level in Dairy Cows with Mastitis. Vet Res Commun 29, 27–34 (2005). https://doi.org/10.1023/B:VERC.0000046740.59694.5d
Issue Date:
DOI: https://doi.org/10.1023/B:VERC.0000046740.59694.5d