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Plant Cell, Tissue and Organ Culture

, Volume 79, Issue 1, pp 53–61 | Cite as

SERK Gene Homolog Expression, Polyamines and Amino Acids Associated with Somatic Embryogenic Competence of Ocotea catharinensis Mez. (Lauraceae)

  • Claudete Santa-Catarina
  • Luiz Ricardo Hanai
  • Marcelo C. Dornelas
  • Ana Maria Viana
  • Eny I.S. Floh
Article

Abstract

In the present work, we investigate the association of SERK gene homolog expression, polyamines (PAs) and amino acids related to putrescine synthesis (arginine and ornithine) and polyamines degradation (γ-aminobutiric acid) or S-adenosylmethionine synthesis (methionine), with the embryogenic competence in cell aggregates of Ocotea catharinensis Mez. (Lauraceae). Cell aggregates were cultivated during 7 days in woody plant medium (WPM) supplemented with 20 g l−1 sucrose, 22 g l−1 sorbitol, 400 mg l−1 glutamine and 2 g l−1 phytagel, and in Murashige and Skoog medium (MS) supplemented 20 g l−1 sucrose, 3 g l−1 activated charcoal, 2 g l−1Phytagel with and without 40 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D). The cell aggregates cultivated in MS plus 2,4-D and in the WPM medium showed hybridization with a SERK gene homolog both in northern and in situ hybridization experiments. Cell aggregates cultivated in an MS basal medium, without 2,4-D, did not exhibit any hybridization signal to the SERK probe used, thus they were considered potentially non-embryogenic cells. In all three media only free polyamines were detected. The higher putrescine levels occurring in WPM callus were associated with a higher arginine and ornithine content, lower γ-aminobutiric acid level, and SERK homolog expression. Putrescine was also the major polyamine in the MS medium. In the MS plus 2,4-D medium, the levels of putrescine, spermidine and spermine were similar. Spermine exhibited similar and the lowest levels in all media. Spermidine intermediary levels occurred in the WPM and MS media. In cell aggregates methionine level was lowest in the MS plus 2,4-D medium, but similar in the MS and WPM media.

γ-aminobutiric acid arginine cell aggregates competence methionine ornithine putrescine spermidine spermine 

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References

  1. Altman A (1989) Polyamines and plant hormones. In: Bachrach U & Heimer VM (eds) The Physiology of Polyamines, Vol. 2 (pp. 122–145). CRC Press, Boca Raton, FL, USAGoogle Scholar
  2. Altman A, Nadel B, Falash Z & Levin N (1990) Somatic embryo-genesis in celery: induction, control and changes in polyamines and proteins. In: Nijkamp HJJ, Van Der Plas LHW & Van Aartrijk J (eds) Progress in Plant Cellular and Molecular Biology (pp. 454–459). Kluwer Academic Publishers, Dordrecht, The NetherlandsGoogle Scholar
  3. Andersen SI, Bastola DR & Minocha SC (1998) Metabolism of polyamines in transgenic cells of carrot expressing a mouse ornithine decarboxylase cDNA. Plant Physiol. 116: 299–307PubMedGoogle Scholar
  4. Astarita LV, Handro W & Floh EIS (2003) Changes in polyamines content associated with zygotic embryogenesis in the Brazilian pine, Araucaria angustifolia (Bert.) O. Ktze. Rev. Bras. Bot. 2: 163–168Google Scholar
  5. Astarita LV, Floh EIS & Handro W (2004) Free amino acid, protein and water content changes associated with seed development in Araucaria angustifolia. Biol. Plant. 47: 53–59Google Scholar
  6. Bais HP & Ravishankar GA (2002) Role of polyamines in the ontogeny of plant and their biotechnology applications. Plant Cell Tiss. Org. Cult. 69: 1–34Google Scholar
  7. Bajaj S & Rajam MV (1995) Efficient plant regeneration from long-term callus cultures of rice by spermidine. Plant Cell Rep. 14: 717–720Google Scholar
  8. Bastola DR & Minocha SC (1995) Increased putrescine biosynthesis through transfer of mouse ornithine decarboxylase cDNA in carrot promotes somatic embryogenesis. Plant Physiol. 109: 63–71PubMedGoogle Scholar
  9. Becraft PW (1998) Receptor kinases in plant development. Trends Plant Sci. 3: 384–388Google Scholar
  10. Boucherau A, Aziz A, Larher F & Martin-Tanguy J (1999) Polyamines and environmental challenges: recent development. Plant Sci. 140: 103–125Google Scholar
  11. Bradley PM, El-Fiji F & Giles KL (1984) Polyamines and arginine affect somatic embryogenesis of Daucus carota. Plant Sci. Lett. 34: 397–401Google Scholar
  12. Claparols I, Santos MA & Torné JM (1993) Influence of some exogenous amino acids on the production of maize embryogenic callus and endogenous amino acid content. Plant Cell Tiss. Org. Cult. 34: 1–11Google Scholar
  13. Dornelas MC, Van Lammeren AA & Kreis M (2000) Arabidopsis thaliana SHAGGY-related protein kinases (AtSK11 and 12) function in perianth and gynoecium development. Plant J. 21: 419–429PubMedGoogle Scholar
  14. Dudits D, Gyorgyey J, Bogre L & Bakó L (1995) Molecular biology of somatic embryogenesis. In: Thorpe TA (ed) In Vitro Embryo-genesis in Plants (pp. 267–308). Kluwer Academic Publisher, Dordrecht, The NetherlandsGoogle Scholar
  15. Fehér A, Pasternak TA & Dudits D (2003) Transition of somatic plant cells to an embryogenic state. Plant Cell Tiss. Org. Cult. 74: 201–228Google Scholar
  16. Feirer RP, Mignon G & Litway JD (1984) Arginine descarboxylase and polyamines required for embryogenesis in the wild carrot. Science 223: 1433–1435Google Scholar
  17. Galston AW & Flores HE (1991) Polyamines and plant morpho-genesis. In: Slocum RD & Flores HE (eds) Biochemistry and Physiology of Polyamines in Plants (pp. 175–186). CRC Press, Boca Raton, FL, USAGoogle Scholar
  18. Haussman JF, Kevers C, Evers D & Gaspar T (1997) Conversion of putrescine in aminobutyric acid, an essential pathway for root formation by poplar shoots in vitro. In: Altman A & Waisel Y (eds) Biology of Root Formation and Development. (pp. 133–140). Plenum Press, New York, USAGoogle Scholar
  19. Hecht V, Vielle-Calzada JP, Hartog MV, Schmidt EDL, Boutilier K, Grossniklaus U & De Vries SC (2001) The Arabidopsis somatic embryogenesis receptor kinase 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture. Plant Physiol. 127: 803–816PubMedGoogle Scholar
  20. Kakkar RK & Sawhney VK (2002) Polyamine research in plants -- a changing perspective. Physiol. Plant. 116: 281–292Google Scholar
  21. Kakkar RK, Nagar PK, Ahuja PS & Raí VK (2000) Polyamines and plant morphogenesis. Biol. Plan 43: 1–11Google Scholar
  22. Kevers C, Le Gal N, Monteiro M, Dommes J & Gaspar T (2000) Somatic embryogenesis of Panax ginseng in liquid cultures: a role for polyamines and their metabolic pathways. Plant Growth Regulat. 31: 209–214Google Scholar
  23. Kong L, Atree SM & Fowke LC (1998) Effects of polyethylene glycol and methylglyoxal bis(guanylhydrazone) on endogenous polyamine levels and somatic embryo maturation in white spruce (Picea glauca). Plant Sci. 133: 211–220Google Scholar
  24. Lee TM, Lur HS & Chu C (1997) Role of abscisic acid in chilling tolerance of rice (Oryza sativa L.) seedlings. II. Modulation of free polyamine levels. Plant Sci. 126: 1–10Google Scholar
  25. Lloyd G & McCown B (1981) Commercially feasible micro-propagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Int. Plant Prop. Soc. Proc. 30: 421–427Google Scholar
  26. Martin-Tanguy J (1997) Conjugated polyamines and reproductive development: biochemical, molecular, and physiological approaches. Physiol. Plant. 100: 675–688Google Scholar
  27. McCabe PF, Valentine TA, Forsberg LS & Pennell RI (1997) Soluble signals from cells identified at the cell wall establish a developmental pathway in carrot. Plant Cell. 9: 2225–2241PubMedGoogle Scholar
  28. Minocha SC & Minocha R (1995) Roles of polyamines in somatic embryogenesis. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol. 30. Somatic Embryogenesis and Synthetic Seeds 1. (pp. 53–70). Springer-Verlag, Berlin, GermanyGoogle Scholar
  29. Minocha R, Smith DR, Reeves C, Steele KD & Minocha SC (1999) Polyamine levels during the development of zygotic and somatic embryos of Pinus radiata. Physiol. Plant. 105: 155–164Google Scholar
  30. Mordhost AP, Toonen MAJ & De Vries SC (1997) Plant embryo-genesis. Crit. Rev. Plant Sci. 16: 535–576Google Scholar
  31. Moura-Costa PH, Viana AM & Mantell SH (1993) In vitro plantlet regeneration of Ocotea catharinensis Mez. (Lauraceae), an endangered forest tree of S. Brasil. Plant Cell Tiss. Org. Cult. 35: 279–286Google Scholar
  32. Murashigue T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497Google Scholar
  33. Nolan KE, Irwanto RR & Rose RJ (2003) Auxin up-regulates MtSERK1 expression in both Medicago trunculata root-forming and embryogenic cultures. Plant Physiol. 133: 218–230PubMedGoogle Scholar
  34. Pennel RI, Janniche L, Scofield GN, Booij H, de Vries SC & Roberts K (1992) Identification of a transitional cell state in the developmental pathway to carrot embryogenesis. J. Cell Biol. 119: 1371–1380PubMedGoogle Scholar
  35. Rajesh MK, Radha E, Karun A & Parthasarathy VA (2003) Plant regeneration from embryo-derived callus of oil palm -- the effect of exogenous polyamines. Plant Cell Tiss. Org. Cult. 75: 41–47Google Scholar
  36. Rey M, Diaz-Sala C & Rodriguez R (1994) Exogenous polyamines improve rooting of hazel microshoots. Plant Cell Tiss. Org. Cult. 36: 303–308Google Scholar
  37. Sambrook J, Fritsch EF & Maniatis T (1989) Molecular Cloning, A Laboratory Manual, 3 vols. Cold Spring Harbour Laboratory Press, Cold Spring Harbour, NY, USAGoogle Scholar
  38. Santos M, Clapatols I & Torné JM (1993) Effect exogenous arginine, ornithine, methionine and γ-amino butyric acid on maize (Zea mays L.) embryogenesis, and polyamine content. J. Plant Physiol. 142: 74–80Google Scholar
  39. Schmidt EDL, Guzzo F, Toonen MAJ & De Vries SC (1997) A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos. Development 124: 2049–2062PubMedGoogle Scholar
  40. Shen H & Galston AW (1985) Correlations between polyamine ratios and growth patterns in seedling roots. Plant Growth Regulat. 3: 353–363Google Scholar
  41. Silveira V, Floh EIS, Handro W & Guerra MP (2004) Effect of plant growth regulators on the cellular growth and levels of intracellular protein, starch and polyamines in embryogenic suspension cultures of Pinus taeda. Plant Cell Tiss. Org. Cult. 76: 53–60Google Scholar
  42. Slocum RD & Flores HE (eds) (1991) Biochemistry and Physiology of Polyamines in Plants. CRC Press, Boca Raton, FL, USAGoogle Scholar
  43. Somleva MN, Scmidt EDL & de Vries SC (2000) Embryogenic cells in Dactylis glomeranta L. (Poaceae) explants identified by cell tracking and by SERK expression. Plant Cell Rep. 19: 718–726Google Scholar
  44. Tiburcio AF, Figueras X, Claparols I, Santos M & Torne JM (1990) Improved plant regeneration in maize callus cultures after pre-treatment with DL-alpha-difluoromethylarginine. Plant Cell Tiss. Org. Cult. 27: 27–32Google Scholar
  45. Toonen MAJ, Schmidt EDL, Van Kammen A & De Vries SC (1997a) Promotive and inhibitory effects of diverse ara-binogalactan proteins on Daucus carota L. somatic embryogenesis. Planta 203: 188–195Google Scholar
  46. Toonen MAJ, Verhees JA, Schmidt EDL, Van Kammen A & De Vries SC (1997b) AtLTP1 luciferase expression during carrot somatic embryogenesis. Plant. J. 12: 1213–1221PubMedGoogle Scholar
  47. Vain P, Flament P & Soudain P (1989) Role of ethylene in embryo-genic callus initiation and regeneration in Zea mays L. J. Plant Physiol. 135: 537–540Google Scholar
  48. Viana AM (1998) Somatic embryogenesis in Ocotea catharinensis Mez (Lauraceae). In: Mantell SH, Bruns S, Tragardh C & Viana AM (eds) Recent Advances in Biotechnology for Conservation and Management (pp. 244–253). International Foundation for Science, Stockholm, SwedenGoogle Scholar
  49. Viana AM & Mantell H (1999) Somatic embryogenesis of Ocotea catharinensis: an endangered tree of the Mata Atlântica (S. Brazil). In: Jain S, Gupta P & Newton R (eds) Somatic Embryogenesis in Woody Plants, Vol. 5 (pp. 3–30). Kluwer Publishers, The NetherlandsGoogle Scholar
  50. Viana AM, Mazza MC & Mantell SH (1999) Plant conservation biotechnology: applications of biotechnology for the conservation and sustainable exploitation of plants from Brazilian rain forest. In: Benson E (ed) Plant Conservation Biotechnology (pp. 277–299). University of Abertay, Dundee, UKGoogle Scholar
  51. Yadav JS & Rajam MV (1997) Spatial distribution of free and conjugated polyamines in leaves of Solanum melongena L. associated with differential morphogenetic capacity: efficient somatic embryogenesis with putrescine. J. Exp. Bot. 48: 1537–1545Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Claudete Santa-Catarina
    • 1
  • Luiz Ricardo Hanai
    • 2
  • Marcelo C. Dornelas
    • 3
  • Ana Maria Viana
    • 4
  • Eny I.S. Floh
    • 1
  1. 1.Plant Cell Biology Laboratory, Department of Botany, Institute of BiosciencesUniversity of São PauloSão PauloBrazil
  2. 2.Departamento de Genética, P.O. BoxUniversidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”PiracicabaBrazil
  3. 3.Plant Biotechnology Laboratory, CENAUniversity of São PauloPiracicabaBrazil
  4. 4.Department of BotanyUniversity Federal of Santa CatarinaFlorianópolisBrazil

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