Plant Cell, Tissue and Organ Culture

, Volume 79, Issue 1, pp 39–44 | Cite as

Effect of Combined Changes in Culture Medium and Incubation Conditions on the Regeneration from Immature Embryos of Elite Varieties of Winter Wheat

  • Cecília Tamás
  • Péter Szűcs
  • Mariann Rakszegi
  • László Tamás
  • Zoltán Bedő


In this study, tissue culture method for plant regeneration from immature embryos of elite Hungarian winter wheat varieties was established. The influence of the growth regulators and the concentration of macroelements in the regeneration medium and of the incubation temperature and light intensity on regeneration frequency were investigated. The most noticeable effect on regeneration frequency was achieved by simultaneously reducing both the incubation temperature to 23 °C and the concentration of macroelements in the regeneration medium to half-strength. This modification increased the average regeneration frequency from about 10–78%. Changes in the light intensity and temperature gave an average plant regeneration frequency of 83%.

immature embryo in vitro culture light shoot regeneration temperature Triticum aestivum L. 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Cecília Tamás
    • 1
  • Péter Szűcs
    • 1
  • Mariann Rakszegi
    • 1
  • László Tamás
    • 2
  • Zoltán Bedő
    • 1
  1. 1.Agricultural Research Institute of the Hungarian Academy of SciencesHungary
  2. 2.Department of Plant PhysiologyEötvös Loránd UniversityHungary

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