Plant Cell, Tissue and Organ Culture

, Volume 79, Issue 1, pp 105–107 | Cite as

Effects of Auxins and 2,3,5-Triiodobenzoic Acid on Somatic Embryo Initiation from Norway Spruce Zygotic Embryos (Picea abies)

  • A.V. Ramarosandratana
  • J. Van Staden


Embryonal-suspensor mass (ESM) initiation from zygotic embryo sections was not affected by explant orientation whatever the type of external auxin used. Thus, polar auxin transport might not be directly involved in the control of somatic embryo formation. Application of 40 µM 2,3,5-triiodobenzoic acid (TIBA) suppressed ESM initiation in hypocotyl sections. This effect of TIBA mimicked that of a supraoptimal dose of α-naphtaleneacetic acid suggesting a detrimental effect of fast internal auxin accumulation on ESM initiation.

conifer polar auxin transport somatic embryogenesis 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • A.V. Ramarosandratana
    • 1
  • J. Van Staden
    • 1
  1. 1.Research Centre for Plant Growth and DevelopmentUniversity of KwaZulu-Natal PietermaritzburgScottsvilleSouth Africa

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