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Plant Cell, Tissue and Organ Culture

, Volume 79, Issue 1, pp 105–107 | Cite as

Effects of Auxins and 2,3,5-Triiodobenzoic Acid on Somatic Embryo Initiation from Norway Spruce Zygotic Embryos (Picea abies)

  • A.V. Ramarosandratana
  • J. Van Staden
Article

Abstract

Embryonal-suspensor mass (ESM) initiation from zygotic embryo sections was not affected by explant orientation whatever the type of external auxin used. Thus, polar auxin transport might not be directly involved in the control of somatic embryo formation. Application of 40 µM 2,3,5-triiodobenzoic acid (TIBA) suppressed ESM initiation in hypocotyl sections. This effect of TIBA mimicked that of a supraoptimal dose of α-naphtaleneacetic acid suggesting a detrimental effect of fast internal auxin accumulation on ESM initiation.

conifer polar auxin transport somatic embryogenesis 

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References

  1. Bronsema FBF, van Oosteveen WJF & van Lammeren AAM (2001) Influence of 2,4-D, TIBA and 3,5-D on the growth response of cultured maize embryos. Plant Cell Tiss. Org. Cult. 65: 45–46Google Scholar
  2. Delbarre A, Muller P, Imhoff V & Guern J (1996) Comparison of mechanisms controlling uptake and accumulation of 2,4-dichlorophenoxy acetic acid, naphtalene-1-acetic acid, and indole-3-acetic acid in suspension-cultured tobacco cells. Planta 198: 532–541Google Scholar
  3. Petrášek J, Elčkner M, Morris D & Zažímalová E (2002) Auxin efflux carrier activity and auxin accumulation regulate cell division and polarity in tobacco cells. Planta 216: 302–308PubMedGoogle Scholar
  4. Ramarosandratana AV & Van Staden J (2002) Tri-iodobenzoic acid promotes embryonal-suspensor mass (ESM) initiation from hypocotyl segments of Norway spruce embryos. In: Proceedings of the 10th IAPTC&B Congress (p. 152A), Orlando, Florida, USAGoogle Scholar
  5. Ramarosandratana AV & Van Staden J (2003) Tissue position, explant orientation and naphtaleneacetic acid (NAA) affect initiation of somatic embryos and callus proliferation in Norway spruce (Picea abies). Plant Cell Tiss. Org. Cult. 74: 249–255Google Scholar
  6. Tokuji Y & Kuriyama K (2003) Involvement of gibberellin and cytokinin in the formation of embryogenic clumps in carrot (Daucus carota). J. Plant Physiol. 160: 133–141PubMedGoogle Scholar
  7. Von Arnold S, Egertsdotter U, Ekberg I, Gupta P, Mo H & Nörgaard J (1995) Somatic embryogenesis in Norway spruce (Picea abies). In: Jain S, Gupta P & Newton R (eds) Somatic Embryogenesis in Woody Plants, Vol. 1 (pp. 17–36). Kluwer Academic Publishers, Dordrecht, The NetherlandsGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • A.V. Ramarosandratana
    • 1
  • J. Van Staden
    • 1
  1. 1.Research Centre for Plant Growth and DevelopmentUniversity of KwaZulu-Natal PietermaritzburgScottsvilleSouth Africa

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