Plant Cell, Tissue and Organ Culture

, Volume 79, Issue 1, pp 95–100 | Cite as

High Frequency Plant Regeneration from Astragalus melilotoides hypocotyl and stem explants via somatic embryogenesis and organogenesis

  • Sui-Wen Hou
  • Jing-Fen Jia


An efficient and reproducible procedure is established for the plant regeneration from hypocotyl explants and hypocotyl-or stem-derived calli in Astragalus melilotoides. High frequency somatic embryo formation (98.3%) occurred direct on hypocotyls on Murashige and Skoog (MS) medium supplemented with 2.69 µM NAA and 4.44 µM BA within 5 weeks. Three types of calli were induced from the hypocotyl and stem segments on MS medium containing 9.05 µM 2,4-D and 2.22–4.44 µM BA. Both somatic embryos and adventitious buds were initiated from hypocotyl-derived calli while only adventitious buds were formed from stem-derived calli in MS medium supplemented with 2.69 µM NAA and 4.44–8.89 µM BA. Somatic embryos or adventitious buds developed into plantlets following being cultured for 3 weeks on MS medium without any growth regulators or with 14.78 µM IBA, respectively. All the regenerated plants were normal with respect to morphology and growth characters, and produced fertile seeds after planting in soil.

Astragalus melilotoides callus induction direct somatic embryogenesis organogenesis plant regeneration 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Sui-Wen Hou
    • 1
  • Jing-Fen Jia
    • 1
  1. 1.School of Life ScienceLanzhou UniversityLanzhouP.R. China

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