Plant Cell, Tissue and Organ Culture

, Volume 79, Issue 1, pp 19–24 | Cite as

Somatic embryogenesis in Centella asiatica L. an important medicinal and neutraceutical plant of India

  • Ch. Paramageetham
  • G. Prasad Babu
  • J.V.S. Rao


Leaf segments excised from Centella asiatica, a medicinal and neutraceutical plant, produced abundant somaticembryoswhen cultured onMS mediumwith 9.29 μMkinetin in combination with 2.26 μM2,4-D. Granular, white,shiny clusters of callus developed after 1 week of culture, and then formed heart and cotyledonary stage embryoson the same medium after 4 weeks. Somatic embryos matured and germinated in the presence of MS mediumcontaining 2.32 μM kinetin with (2.89μM) GA3. Plantlets were successfully transferred to pots containing amixture of soil and vermiculite (1:1).

Apiaceae asiaticosides bipolar and unipolar embryos leaf explants 


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  1. Ahmad Ru (1993) Medicinal plants used in ISM -- their procurement, cultivation, regeneration and import/export aspects: a review. In: Govil JN, Singh VK & Hashmi S (eds) Medicinal Plants: New Vistas of Research. Part I (pp 221–258). Today and Tomorrow printers and publishers, New Delhi.Google Scholar
  2. Banerjee S, Zehra M & Kumar S (1999) In vitro multiplication of Centella asiatica, a medicinal herb from leaf explants. Curr. Sci. 76: 147–148.Google Scholar
  3. Kameshwara Rao C (ed) (2000) In: Material for the Database of Medicinal Plants. Karnataka State council for Science and Technology for the Dept. of Forests, Environment and Ecology Govt. of Karnataka. 90 pp.Google Scholar
  4. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15: 473–497.Google Scholar
  5. Pandey NK, Tewari KC, Tewari RN, Joshi GC, Pande VN & Pandey G (1993). Medicinal plants of Kumaon Himalaya strategies for conservation. In: Dhar U (ed) Himalayan Biodiversity Conservation Strategies, No. 3 (pp 293–302) Himavikas Publications, Nanital.Google Scholar
  6. Patra A, Rai B, Rout GR & Das P (1998) Successful plant regeneration from callus cultures of Centella asiatica (Linn.) Urban. Plant Growth Regul. 24: 13–16.Google Scholar
  7. Prakash E, Shavalli Khan, Elusing Meru & Rao KR (2001) Somatic embryogenesis in Pimpinella tirupatiensis Bal and Subr., an endangered medicinal plant of Tirumala hills. Curr. Sci. 81(9): 1239–1242.Google Scholar
  8. Samantaray S, Rout GR & Das P (1997) Regeneration of plants via somatic embryogenesis from leaf base and leaf tip segments of Echinochloa colona. Plant Cell Tiss. Org. Cult. 47: 119–125.Google Scholar
  9. Sharma BL & Kumar A (1998) Biodiversity of Medicinal plants of Trigugi Narain (Garhwal Himalaya) and their conservation. National Conference on Recent Trends in Spices and Medicinal Plant Research, A-78 (2–4) April, Calcutta, WB, India.Google Scholar
  10. Singh HG (1989) Himalayan herbs and drugs importance and extinction threat. J. Sci. Res. Plants Med. 10: 47–52.Google Scholar
  11. Srivastava R, Shukla YN & Kumar S (1998) Chemistry and pharmacology of Centella asiatica: a review. J. Med. Arom. Plant Sci. 19: 1049–1056.Google Scholar
  12. Tanaka K, Kanno Y, Kudo S & Suzuki M (2000) Somatic embryogenesis and plant regeneration in Chrysanthemum (Dendanthema grandiflorum (Ramt.) Kitamura). Plant Cell Rep. 19: 946–953.Google Scholar
  13. Tiwari V, Singh BD & Tiwari KN (1998) Shoot regeneration and somatic embryogenesis from different explants of brahmi (Bacopa monniera L. wettst). Plant Cell Rep. 17: 538–543.Google Scholar
  14. Tiwari KN, Sharma NC, Tivari V & Singh BD (2000) Micropropagation of Centella asiatica (L.), a valuable medicinal herb. Plant Cell Tiss. Org. Cult. 63: 179–185.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Ch. Paramageetham
    • 1
  • G. Prasad Babu
    • 1
  • J.V.S. Rao
    • 1
  1. 1.Plant Biotechnology Laboratory, Department of BotanyS.V. UniversityTirupati-India

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