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Plant Cell, Tissue and Organ Culture

, Volume 79, Issue 1, pp 19–24 | Cite as

Somatic embryogenesis in Centella asiatica L. an important medicinal and neutraceutical plant of India

  • Ch. Paramageetham
  • G. Prasad Babu
  • J.V.S. Rao
Article

Abstract

Leaf segments excised from Centella asiatica, a medicinal and neutraceutical plant, produced abundant somaticembryoswhen cultured onMS mediumwith 9.29 μMkinetin in combination with 2.26 μM2,4-D. Granular, white,shiny clusters of callus developed after 1 week of culture, and then formed heart and cotyledonary stage embryoson the same medium after 4 weeks. Somatic embryos matured and germinated in the presence of MS mediumcontaining 2.32 μM kinetin with (2.89μM) GA3. Plantlets were successfully transferred to pots containing amixture of soil and vermiculite (1:1).

Apiaceae asiaticosides bipolar and unipolar embryos leaf explants 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Ch. Paramageetham
    • 1
  • G. Prasad Babu
    • 1
  • J.V.S. Rao
    • 1
  1. 1.Plant Biotechnology Laboratory, Department of BotanyS.V. UniversityTirupati-India

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