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Somatic Cell and Molecular Genetics

, Volume 25, Issue 1, pp 49–57 | Cite as

Introduction of Plasmid DNA and Oligonucleotides into Lung Epithelial Cells by the Hemagglutinating Virus of Japan (HVJ)-Liposome Method

  • Mitsuhiro Yoshida
  • Seiji HayashiEmail author
  • Junko Sakuma-Mochizuki
  • Kin'nya Abe
  • Toru Arai
  • Masahide Mori
  • Sho Goya
  • Hiroto Matsuoka
  • Yasufumi Kaneda
  • Tadamitsu Kishimoto
Article
  • 22 Downloads

Abstract

The hemagglutinating virus of Japan (HVJ) fused with liposomes provides a unique transfection vehicle with characteristics of both virus vector and liposome. Here we investigate the efficiency and safety of the HVJ-liposome technique in delivering foreign genes and oligonucleotides into the lung of the Wistar rat. A plasmid vector containing the Escherichia coli β-galactosidase (β-gal) gene and the chicken β-actin promoter was transfected via the trachea using the HVJ-liposome method. Cytochemical staining showed expression of exogenous β-gal activity in airway epithelial cells, alveolar macrophages, and alveolar type II cells. This activity persisted at least 28 days after administration of the genes. FITC-labeled oligonucleotides also were introduced into the same types of lung cells as those expressing β-gal. After instillation of HVJ-liposome, anti-HVJ antibodies were detected in the sera of the rats, but even after repeated administration of HVJ-liposome, no marked histopathologic change was observed while exogenous β-gal expression was detected in pulmonary cells.

Keywords

Escherichia Coli Alveolar Macrophage Virus Vector Plasmid Vector Histopathologic Change 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1999

Authors and Affiliations

  • Mitsuhiro Yoshida
    • 1
  • Seiji Hayashi
    • 1
    Email author
  • Junko Sakuma-Mochizuki
    • 1
  • Kin'nya Abe
    • 1
  • Toru Arai
    • 1
  • Masahide Mori
    • 1
  • Sho Goya
    • 1
  • Hiroto Matsuoka
    • 1
  • Yasufumi Kaneda
    • 2
  • Tadamitsu Kishimoto
    • 3
  1. 1.Department of Molecular MedicineOsaka University Medical SchoolOsakaJapan
  2. 2.Division of Gene Therapy ScienceOsaka University Medical SchoolOsakaJapan
  3. 3.Osaka UniversityOsakaJapan

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