Somatic Cell and Molecular Genetics

, Volume 25, Issue 1, pp 49–57 | Cite as

Introduction of Plasmid DNA and Oligonucleotides into Lung Epithelial Cells by the Hemagglutinating Virus of Japan (HVJ)-Liposome Method

  • Mitsuhiro Yoshida
  • Seiji HayashiEmail author
  • Junko Sakuma-Mochizuki
  • Kin'nya Abe
  • Toru Arai
  • Masahide Mori
  • Sho Goya
  • Hiroto Matsuoka
  • Yasufumi Kaneda
  • Tadamitsu Kishimoto


The hemagglutinating virus of Japan (HVJ) fused with liposomes provides a unique transfection vehicle with characteristics of both virus vector and liposome. Here we investigate the efficiency and safety of the HVJ-liposome technique in delivering foreign genes and oligonucleotides into the lung of the Wistar rat. A plasmid vector containing the Escherichia coli β-galactosidase (β-gal) gene and the chicken β-actin promoter was transfected via the trachea using the HVJ-liposome method. Cytochemical staining showed expression of exogenous β-gal activity in airway epithelial cells, alveolar macrophages, and alveolar type II cells. This activity persisted at least 28 days after administration of the genes. FITC-labeled oligonucleotides also were introduced into the same types of lung cells as those expressing β-gal. After instillation of HVJ-liposome, anti-HVJ antibodies were detected in the sera of the rats, but even after repeated administration of HVJ-liposome, no marked histopathologic change was observed while exogenous β-gal expression was detected in pulmonary cells.


Escherichia Coli Alveolar Macrophage Virus Vector Plasmid Vector Histopathologic Change 
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Copyright information

© Plenum Publishing Corporation 1999

Authors and Affiliations

  • Mitsuhiro Yoshida
    • 1
  • Seiji Hayashi
    • 1
    Email author
  • Junko Sakuma-Mochizuki
    • 1
  • Kin'nya Abe
    • 1
  • Toru Arai
    • 1
  • Masahide Mori
    • 1
  • Sho Goya
    • 1
  • Hiroto Matsuoka
    • 1
  • Yasufumi Kaneda
    • 2
  • Tadamitsu Kishimoto
    • 3
  1. 1.Department of Molecular MedicineOsaka University Medical SchoolOsakaJapan
  2. 2.Division of Gene Therapy ScienceOsaka University Medical SchoolOsakaJapan
  3. 3.Osaka UniversityOsakaJapan

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