Somatic Cell and Molecular Genetics

, Volume 25, Issue 1, pp 1–8 | Cite as

Phosphatase Inhibitors and Premature Chromosome Condensation in Human Peripheral Lymphocytes at Different Cell-Cycle Phases

  • Reiko KandaEmail author
  • Kiyomi Eguchi-Kasai
  • Isamu Hayata


The cytogenetical reaction of human peripheral lymphocytes to okadaic acid and calyculin A was examined. Calyculin A could induce PCC about 20 times more effectively than okadaic acid. Their mechanisms of PCC induction were judged similar by their dose-dependent manner and chromosome morphology. Contrary to earlier studies suggesting that chemicals could not induce PCC in G 1 cells where little cyclin B is present, the present study showed that calyculin A could induce PCC in lymphocytes not only at S and G 2 /M but also at the second G 1 phase after PHA stimulation in vitro. PCC was induced slightly in lymphocytes both at G 0 and the first in vitro G 1 phase even when the calyculin A concentration increased one hundred fold. It was found that calcium ionophore A23187 increased frequencies of G 0 -PCC induced by calyculin A, although a further refinement is necessary to obtain a suitable morphology of G 0 -PCC for cytogenetic studies.


Calcium Phosphatase Inhibitor Okadaic Acid Cytogenetic Study Peripheral Lymphocyte 
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Copyright information

© Plenum Publishing Corporation 1999

Authors and Affiliations

  • Reiko Kanda
    • 1
    Email author
  • Kiyomi Eguchi-Kasai
    • 1
  • Isamu Hayata
    • 1
  1. 1.Division of Radiobiology and BiodosimetryNational Institute of Radiological SciencesChibaJapan.

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