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Somatic Cell and Molecular Genetics

, Volume 25, Issue 1, pp 1–8 | Cite as

Phosphatase Inhibitors and Premature Chromosome Condensation in Human Peripheral Lymphocytes at Different Cell-Cycle Phases

  • Reiko KandaEmail author
  • Kiyomi Eguchi-Kasai
  • Isamu Hayata
Article

Abstract

The cytogenetical reaction of human peripheral lymphocytes to okadaic acid and calyculin A was examined. Calyculin A could induce PCC about 20 times more effectively than okadaic acid. Their mechanisms of PCC induction were judged similar by their dose-dependent manner and chromosome morphology. Contrary to earlier studies suggesting that chemicals could not induce PCC in G 1 cells where little cyclin B is present, the present study showed that calyculin A could induce PCC in lymphocytes not only at S and G 2 /M but also at the second G 1 phase after PHA stimulation in vitro. PCC was induced slightly in lymphocytes both at G 0 and the first in vitro G 1 phase even when the calyculin A concentration increased one hundred fold. It was found that calcium ionophore A23187 increased frequencies of G 0 -PCC induced by calyculin A, although a further refinement is necessary to obtain a suitable morphology of G 0 -PCC for cytogenetic studies.

Keywords

Calcium Phosphatase Inhibitor Okadaic Acid Cytogenetic Study Peripheral Lymphocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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LITERATURE CITED

  1. 1.
    Gotoh, E., Asakawa, Y., and Kosaka, H. (1995). Inhibition of protein serine/threonine phosphatases directly induces premature chromosome condensation in mammalian cells. Biomed. Res. 16:63–68.Google Scholar
  2. 2.
    Gotoh, E., and Asakawa, Y. (1996). Detection and evaluation of chromosomal aberrations induced by high doses of γ-irradiation using immunogold-silver painting of prematurely condensed chromosomes. Int. J. Radiat. Biol. 70:517–520.Google Scholar
  3. 3.
    Kanda, R., Hayata, I., and Lloyd, D.C. (1999). Easy biodosimetry for high-dose radiation exposures using drug-induced prematurely condensed chromosomes. Int. J. Radiat. Biol. 75:441–446.Google Scholar
  4. 4.
    Durante, M., Furusawa, Y., and Gotoh, E. (1998). A simple method for simultaneous interphase-metaphase chromosome analysis in biodosimetry. Int. J. Radiat. Biol. 74:457–462.Google Scholar
  5. 5.
    Ghosh, S., Paweletz, N., and Schroeter, D. (1992). Failure of kinetochore development and mitotic spindle formation in okadaic acid-induced premature mitosis in Hela cells. Exp. Cell Res. 201:535–540.Google Scholar
  6. 6.
    Tam, S.W., and Schlegel, R. (1992). Staurosporine overrides checkpoints for mitotic onset in BHK cells. Cell Growth. Differ. 3:811–817.Google Scholar
  7. 7.
    Tam, S.W., Belinsky, G.S., and Schlegel, R. (1995). Premature expression of cyclin B sensitizes human HT1080 cells to caffeine-induced premature mitosis. J. Cell Biochem. 59:339–349.Google Scholar
  8. 8.
    Yoshida, M., Usui, T., Tsujimura, K., Inagaki, M., Beppu, T., and Horinouchi, S. (1997). Biochemical differences between staurosporine-induced apoptosis and premature mitosis. Exp. Cell Res. 232:225–239.Google Scholar
  9. 9.
    Rao, P.N., Wilson, B., and Puck, T.T. (1977). Premature chromosome condensation and cell cycle analysis. J. Cell Physiol. 91:131–142.Google Scholar
  10. 10.
    Eguchi-Kasai, K., Itsukaichi, H., Murakami, M., Kanai, T., Shimizu, K., and Sato, K. (1996). Effect of exposure of carbon ion beams on cell cycle progression. J. Radiat. Res. 37:309.Google Scholar
  11. 11.
    Guglielmi, G.E., Vogt, T.F., and Tice, R.R. (1982). Induction of sister chromatic exchanges and inhibition of cellular proliferation in vitro. I. Caffeine. Environ. Mutagen. 4:191–200.Google Scholar
  12. 12.
    Beetham, K.L., and Tolmach, L.J. (1982). Growth and death of HeLa cells in the presence of caffeine. J. Cell Physiol. 113:385–397.Google Scholar
  13. 13.
    Pantelias, G.E., and Maillie, H.D. (1984). The use of peripheral blood mononuclear cell prematurely condensed chromosomes for biological dosimetry. Radiat. Res. 99:140–150.Google Scholar
  14. 14.
    Coco-Martin, J.M., and Begg, A.C. (1997). Detection of radiation-induced chromosome aberrations using fluorescence in situ hybridization in drug-induced premature chromosome condensations of tumour cell lines with different radiosensitivities. Int. J. Radiat. Biol. 71:265–273.Google Scholar
  15. 15.
    Dyban, A.P., De Sutter, P., and Verlinsky, Y. (1993). Okadaic acid induces premature chromosome condensation reflecting the cell cycle progression in one-cell stage mouse embryos. Mol. Reprod. Dev. 34:402–415.Google Scholar
  16. 16.
    Steinmann, K.E., Belinsky, G.S., Lee, D., and Schlegel, R. (1991). Chemically induced premature mitosis: Differential response in rodent and human cells and the relationships to cyclin B synthesis and p34cdc2/cyclin B complex formation. Proc. Nat. Acad. Sci. U.S.A. 88:6842–6847.Google Scholar
  17. 17.
    Colomer, J., Lopez-Girona, A., Agell, N., and Bachs, O. (1994). Calmodulin regulates the expression of cdks, cyclins and replicative enzymes during proliferative activation of human T lymphocytes. Biochem. Biophys. Res. Commun. 200:306–312.Google Scholar
  18. 18.
    Wolowiec, D., Benchaib, M., Pernas, P., Deviller, P., Souchier, C., Rimokh, R., Felman, P., Bryon, P.A., and Ffrench, M. (1995). Expression of cell cycle regulatory proteins in chronic lymphocytic leukemias. Comparison with non-Hodgkin's lymphomas and non-neoplastic lymphoid tissue. Leukemia 9:1382–1388.Google Scholar
  19. 19.
    Ciemerych, M.A., Tarkowski, A.K., and Kubiak, J.Z. (1998). Autonomous activation of histone H1 kinase, cortical activity and microtubule organization in one-and two-cell mouse embryos. Biol. Cell 90:557–564.Google Scholar
  20. 20.
    Ajiro, K., Yasuda, H., and Tsuji, H. (1996). Vanadate triggers the transition from chromosome condensation to decondensation in a mitotic mutant (tsTM13) inactivation of p34cdc2/H1 kinase and dephosphorylation of mitosis-specific histone H3. Eur. J. Biochem. 41: 923–930.Google Scholar
  21. 21.
    Maldonado-Codina, G., and Gloverm, D.M. (1992). Cyclins A and B associate with chromatin and the polar regions of spindles, respectively, and do not undergo complete degradation at anaphase in syncytial Drosophila embryos. J. Cell Biol. 116:967–976.Google Scholar
  22. 22.
    Hanks, S.K., Rodriguez, L.V., and Rao, P.N. (1983). Relationship between histone phosphorylation and premature chromosome condensation. Exp. Cell Res. 148:293–302.Google Scholar
  23. 23.
    Guo, X.W., Th'ng, J.P.H., Swank, R.A., Anderson, H.J., Tudan, C., Bradbury, E.M., and Roberge, M. (1995). Chromosome condensation induced by fostriecin does not require p34cdc2 kinase activity and histone H1 hyperphosphorylation, but is associated with enhanced histone H2A and H3 phospholylation. EMBO J. 14:976–985.Google Scholar
  24. 24.
    Ajiro, K., Yoda, K., Utsumi, K., and Nishikawa, Y. (1996) Alteration of cell cycle-dependent histone phosphorylations by okadaic acid. Induction of mitosis-specific H3 phosphorylation and chromatin condensation in mammalian interphase cells. J. Biol. Chem. 271:13197–13201.Google Scholar
  25. 25.
    Van Hooser, A., Goodrich, D.W., Allis, C.D., Brinkley, B.R., and Mancini, M.A. (1998). Histone H3 phosphorylation is required for the initiation, but not maintenance, of mammalian chromosome condensation. J. Cell Sci. 111:3497–3506.Google Scholar
  26. 26.
    Whitlock, J.P. Jr., Augustine, R., and Schulman, H. (1980). Calcium-dependent phosphorylation of histone H3 in butyrate-treated HeLa cells. Nature 287:74–76.Google Scholar
  27. 27.
    Whitlock, J.P. Jr., Galeazzi, D., and Schulman, H. (1983). Acetylation and calcium-dependent phosphorylation of histone H3 in nuclei from butyrate-treated HeLa cells. J. Biol. Chem. 258:1299–1304.Google Scholar
  28. 28.
    Kanda, R., Jiang, T., Hayata, I., and Kobayashi, S. (1994). Effects of colcemid concentration on chromosome aberration analysis in human lymphocytes. J. Radiat. Res. 35:41–47.Google Scholar

Copyright information

© Plenum Publishing Corporation 1999

Authors and Affiliations

  • Reiko Kanda
    • 1
    Email author
  • Kiyomi Eguchi-Kasai
    • 1
  • Isamu Hayata
    • 1
  1. 1.Division of Radiobiology and BiodosimetryNational Institute of Radiological SciencesChibaJapan.

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