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Tryptophan 7-Halogenase from Pseudomonas aureofaciens ACN Strain: Gene Cloning and Sequencing and the Enzyme Expression

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Abstract

The gene of tryptophan 7-halogenase was isolated from the Pseudomonas aureofaciens ACN strain producing pyrrolnitrin, a chlorocontaining antibiotic, and sequenced. A high homology degree (over 95%) was established for the genes and the corresponding halogenases from P. aureofaciens ACN and P. fluorescens BL915. The tryptophan 7-halogenase gene was amplified by PCR, and the corresponding enzyme was expressed in Escherichia coli cells using the pBSII SK+ vector.

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Authors and Affiliations

  1. Kupala State University, per, Dovatora 3/1, Grodno, 230012, Belarus

    V. N. Burd

  2. Technical University, Dresden, Germany

    K.-H. van Pee

Authors
  1. V. N. Burd
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  2. K.-H. van Pee
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Burd, V.N., van Pee, KH. Tryptophan 7-Halogenase from Pseudomonas aureofaciens ACN Strain: Gene Cloning and Sequencing and the Enzyme Expression. Russian Journal of Bioorganic Chemistry 30, 353–358 (2004). https://doi.org/10.1023/B:RUBI.0000037262.79118.20

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  • DOI: https://doi.org/10.1023/B:RUBI.0000037262.79118.20

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