Plant Foods for Human Nutrition

, Volume 58, Issue 3, pp 1–10 | Cite as

Hypolipidemic effect of pulse seed coats in rats

  • Nikita Sihag
  • Asha Kawatra


The present investigation was undertaken to study the hypolipidemic effect of bengal gram and green gram seed coats in rats. Isonitrogenous diets having one percent cholesterol and ten percent dietary fiber from both pulse seed coats were fed to four groups of albino rats for six weeks.The levels of total lipids, cholesterol, LDL-cholesterol and triglycerides in the serum were significantly reduced when cellulose of the diet was replaced by pulse seed coats. However, serum levels of HDL-cholesterol and phospholipids were not significantly affected by pulse seed coats. Fecal analyses indicated that cholesterol and bile acid excretion higher in the bengal gram seed coat fed group than green gram fed groep. Bengal gram seed coats were observed to be more hypolipidemic than green gram seed coats.

Bengal gram seed coat Cholesterol Dietary fiber Green gram seed coat HDL-cholesterol LDL-cholesterol Phospholipids Serum lipids Triglycerides 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Anderson JW, Spencer DB, Hamilton CC, Smith SF, Tietyen J, Bryant CA, Oeltgen P (1990) Oat bran and cereal lower serum total and LDL cholesterol in hypercholester-olemic men. Am J Clin Nutr: 495–499.Google Scholar
  2. 2.
    AOAC (1997) Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists, Washington, D.C.Google Scholar
  3. 3.
    Van Soest PJ, Wines RH (1967) Use of detergents in the analysis of fibrous feeds and determination of plant cell wall constituents. J Assoc Off Anal Chem 50: 50–55.Google Scholar
  4. 4.
    Rocos HG, Farenbach MJ (1963) Analytical biochem 6: 520–529.Google Scholar
  5. 5.
    Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for isolation and purification of total lipids from animal tissues. J Biol Chem: 226–407.Google Scholar
  6. 6.
    Brockhuyse RM (1968) Phospholipids in tissues of eye. Biochem Biophys Acta 152: 307.Google Scholar
  7. 7.
    Friedewald WT, Levy R, Frederickson DPS (1972) Estimation of plasma low density lipoprotein cholesterol concentration without use of preparative ultracentrifuge. Clin Chem 18: 499.Google Scholar
  8. 8.
    Sharma M, Kawatra A (1995) Effect of dietary fibre from cereal brans and pulse seed coats on serum lipids in rats. Pl Fd Hum Nutr 47: 287–292.Google Scholar
  9. 9.
    Uberoi SK, Vadhera S, Soni GL (1992) Role of dietary fibre from pulses and cereals as hypocholesterolemic and hypolipidemic agents. J Fd Sci Technol 29: 281–283.Google Scholar
  10. 10.
    Taneja A, Bhat CM, Arora A, Kaur AP (1989) Effect of incorporation of isabgol huks in a low fibre diet on faecal excretion and serum levels of lipids in adolescent girls. European J Clin Nutr 43: 197–202.Google Scholar
  11. 11.
    Vahouny GV (1982) Dietary fibres and intestinal absorption of lipids. In Vahouny GV, Kritchevsky D (eds), Dietary Fibre in Health and Disease. New York: Plenum Press, pp 203–227.Google Scholar
  12. 12.
    Vadhera S, Punia AK, Soni GL (1995) Hypocholesterolemic/hypolipidemic effect of dietary fibres from outer dry skin of garlic and onion. J Fd Sci Technol 32: 62–64.Google Scholar
  13. 13.
    Agarwal V, Chauhari BM (1988) Effect of composition and hypolipidemic effect of dietary fibre from some plant fonds. Pl Fd Hum Nutr 38: 189–197.Google Scholar
  14. 14.
    Kies C, Fox HM (1977) Dietary hemicellulose interactions influencing serum lipid patterns and protein nutritional status of adult men. J Fd Sci 42: 440–443.Google Scholar
  15. 15.
    Rani B, Kawatra A (1998) Hypolipidemic effect of some plant foods. J Dairying Foods and Home Sci 17(1): 61–65.Google Scholar
  16. 16.
    Chen WJL, Anderson JW (1986) Hypo-cholesterol effect of soluble fibre. In Vahouny GV, Kritchevsky D (eds), Dietary Fibre Basic and Clinical Aspects. New York: Plenum Press, pp 275–286.Google Scholar
  17. 17.
    Kelley JJ, Tsai AC (1978) Effect of pectin, gum arabic and agar on cholesterol absorption, synthesis and turn over in rats. J Nutr 108: 630–639.Google Scholar
  18. 18.
    Kirby RM, Anderson JW, Sieling B, Rees ED, Chen WJL, Miller RE, Kay RM (1981) Oat-bran intake selectively lowers low density lipoprotein cholesterol concentrations of hypercholesterolemic men. Am J Clin Nutr 34: 824–829.Google Scholar
  19. 19.
    Thomas M, Leelam S, Kurup PA (1989) Effect of black gram fibre (Phaseolus mungo) on the metabolism of lipoproteins in rats. J Fd Sci Technol 27: 224–227.Google Scholar
  20. 20.
    Vles RO, Gottenbos JJ (1989) Nutritional characteristics and food uses of vegetable oils. In: Robbelen G, Downey RK, Ashri A (eds), Oil crops of world. New York: McGraw-Hill Publishing Company, pp 63–86.Google Scholar
  21. 21.
    Zingarde F, Larson L (1984) Effect of a concentrated bran fibre preparation on HDL-cholesterol in hypercholesterolemic men. Human Nutr Clin Nutr 38C: 39–45.Google Scholar
  22. 22.
    Jackson KA, Suter DAI, Topping DL (1994) Oatbran barley and malted barley lower plasma cholesterol relative to wheat bran but differ in their effects on fiber cholesterol in rats fed diets with and without cholesterol. J Nutr 124: 1678–1684.Google Scholar
  23. 23.
    Jonnalagadda SS, Thye FW, Robertson JL (1993) Plasma total and lipoprotein cho-lesterol and faecal cholesterol excretion in hamsters fed fibre diets. J Nutr 123: 1377–1382.Google Scholar
  24. 24.
    Wu AL, Clark SB, Holt PR (1980) Composition of lymph chylomicrons from proximal and distal rats small intestine. Am J Clin Nutr 33: 582–589.Google Scholar
  25. 25.
    Zhang JX, Lundin E, Hallmans G, Adlercreutz H, Anderson H, Bosneus I, Aman P, Stenling R, Dahlgren S (1994) Effect of dry bran on excretion of bile acids, cholesterol, nitrogen and fat in human subjects with ileostomies. Am J Clin Nutr 59: 389–394.Google Scholar
  26. 26.
    Anderson JW, Chen WJL (1979) Plant fibre, carbohydrate and lipid metabolism. Am J Clin Nutr 32: 346–363.Google Scholar
  27. 27.
    Usha V, Vijayammal PL, Kurup PA (1984) Effect of dietary fibre from banana (Musa paradisca) on cholesterol metabolism. Ind J Exptal Biol 22: 550.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Nikita Sihag
    • 1
  • Asha Kawatra
    • 1
  1. 1.Department of Foods and NutritionCCS Haryana Agricultural UniversityHisarIndia

Personalised recommendations