Abstract
Cytokinin oxidase/dehydrogenase (EC 1.5.99.12) specific activity was determined in leaves and roots of two P. sativum cultivars (cv. Scinado and cv. Manuela) during vegetative development and the effect of UV-B irradiation or elevated temperature was assessed. The measurement of CKX activity during development showed localisation of this enzyme to roots. The reduction in CKX activity in leaves after UV-B irradiation and the increased levels of the enzyme in high temperature-treated plants suggests that the enzymes from the CKX gene family have a different expression during stress responses provoked by different factors and probably are tissue specific. Differences regarding cytokinin oxidase/dehydrogenase activity stress response were observed between the two pea cultivars.
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References
Auer C.A., Motyka V., B?ezinová A. and Kaminek M. 1999. Endogenous cytokinin accumulation and cytokinin oxidase activity during shoot organogenesis of Petubia hybrida. Physiol. Plant 105: 141–147.
Bradford M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
Buchanan-Wollaston V., Earl S., Harrison E., Mathas E., Navapour S., Page T. and Pink D. 2003. The molecular analysis of leaf senescence-a genomic approach. Plant Biotech. J. 1: 3–22.
Gan S. and Amasino R.M. 1995. Inhibition of leaf senescence by autoregulated production of cytokinin. Science 270: 1986–1988.
Guivarc'h A., Rembur J., Goetz M., Roitsch T., Noin M., Schmülling T. and Chriqui D. 2002. Local expression of the ipt gene in transgenic tobacco (Nicotiana tabacum L. cv. SR1) axillary buds establishes a role for cytokinins in tuberization and sink formation. J. Exp. Bot. 53(369): 621–629.
Jäger A.K., Stirk W.A. and van Staden J. 1997. Cytokinin oxidase activity in habituated and non-habituated soybean callus. Plant Growth Regul. 22: 203–206.
Jones R.J. and Schreiber B.M.N. 1997. Role and function of cytokinin oxidase in plants. Plant Growth Regul. 23: 123–134.
Kaminek M., Motyka V. and Vankova R. 1997. Regulation of cytokinin content in plant cells. Physiol. Plant 101: 689–700.
Liberos-Minotta C.A. and Tipton P.A. 1995. A colorimetric assay for cytokinin oxidase. Anal. Biochem. 231: 339–341.
Mähönen A.P., Bonke M., Kaupinnen L., Riikonen M., Benfey P.N. and Helariutta Y. 2000. A novel two-component hybrid molecule regulates vascular morphogenesis of the Arabidopsis root. Genes Dev. 14: 2938–2943.
Schmülling T. 2002. New insights into the functions of cytokinins in plant development. J Plant Growth Regul. 21: 40–49.
Werner T., Motika V., Strand M. and Schmülling T. 2001. Regulation of plant growth by cytokinin. Proc. Natl. Acad. Sci. USA 98(18): 10487–10492.
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Vaseva-Gemisheva, I., Lee, D., Alexieva, V. et al. Cytokinin oxidase/dehydrogenase in Pisum sativum plants during vegetative development. Influence of UV-B irradiation and high temperature on enzymatic activity. Plant Growth Regulation 42, 1–5 (2004). https://doi.org/10.1023/B:GROW.0000014888.45592.4d
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DOI: https://doi.org/10.1023/B:GROW.0000014888.45592.4d