Advertisement

Cytotechnology

, Volume 24, Issue 1, pp 1–9 | Cite as

Insect cell physiology

  • Ravinder Bhatia
  • Gary Jesionowski
  • Jerome Ferrance
  • Mohammad M. Ataai
Article
insect cells metabolism energetics amino acids fluxes oxygen uptake 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bedard C, Tom R and Kamen A (1993) Growth, nutrient consumption and end-product accumulation in Sf9 and BTI-EAA insect cell cultures: Insights into growth limitation and metabolism. Biotechnology Progress 9: 615–624.PubMedCrossRefGoogle Scholar
  2. Bedard C, Kamen A, Tom R and Massie B (1994) Maximization of recombinant protein yield in the insect cell/baculovirus system by on-time addition of nutrients to high-density batch cultures. Cytotechnology 15: 129–138.PubMedCrossRefGoogle Scholar
  3. Caron AW, Archambaust J and Massie B (1990) High-level recombinant protein production in bioreactors using the baculovirus-insect cell expression system. Biotechnol. and Bioeng. 36: 1133–1140.CrossRefGoogle Scholar
  4. Cho T, Shuler ML and Granados RR (1989) Current developments in new media and cell culture system for the large-scale production of insect cells. Advances in Cell Culture 7: 261–277. Academic Press, New York.Google Scholar
  5. Clements AN and Grace TDC (1967) The utilization of sugars by insect cell in culture. J. Insect Physiology 13: 1327–1332.CrossRefGoogle Scholar
  6. Fraser MJ (1989) Expression of eucaryotic genes in insect cell culture. In vitro cellular and developmental biology 253: 225–235.PubMedGoogle Scholar
  7. Ferrance JP, Goel A and Ataai MM (1993) Utilization of glucose and amino acids in insect cell cultures: Quantifying the metabolic flows within the primary pathways and medium development. Biotechnol. and Bioeng. 42: 697–707.CrossRefGoogle Scholar
  8. Goel A, Domach MM and Ataai MM (1995a) A novel dual feeding strategy for suppression of acid formation in bacteria: Lowering of the glycolysis flux. Biotechnology Progress, in press.Google Scholar
  9. Goel A, Domach MM, Hanley W and Ataai MM (1995b) Coordination of glycolysis and TCA cycle reaction networks: Citrateglucose co-metabolism eliminate acids and reveals potential metabolic engineering strategies. Ann. NY. Acad. Sci., in press.Google Scholar
  10. Grace TDC and Brzostowski HW (1966) Analysis of the amino acids and sugars in an insect culture medium during cell growth. Journal of Insect Physiology 12: 625–633.CrossRefGoogle Scholar
  11. Hensler WT and Agathos SN (1994) Evaluation of monitoring approaches and effects of culture conditions on recombinant protein production in baculovirus-infected cells. Cytotechnology 15: 177–186.PubMedCrossRefGoogle Scholar
  12. Hink WF, Thomsen DR, Davidson DJ and Castellino FJ (1991) Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress 7: 9–14.PubMedCrossRefGoogle Scholar
  13. Kamen AA, Tom RL, Caron AW, Massie B and Archambault J (1991) Culture of insect cells in a helical ribbon impeller bioreactor. Biotechnology and Bioengineering 38: 619–628.CrossRefPubMedGoogle Scholar
  14. Lazarte JE, Tosi PF and Nicolau C (1992) Optimization of the production of full-length rCD4 in baculovirus-infected Sf9 cells. Biotechnol. and Bioeng. 40: 214–217.CrossRefGoogle Scholar
  15. Licari P and Bailey JE (1992) Factors influencing recombinant protein yields in an insect cell-baculovirus system: multiplicity of infection and intracellular protein degradation. Biotechnol. and Bioeng. 39: 614–618.CrossRefGoogle Scholar
  16. Lindsay DA and Betenbaugh MG (1992) Quantification of cell culture factors affecting recombinant protein yields in baculovirus-infected cells. Biotechnol. and Bioeng. 39: 614–618.CrossRefGoogle Scholar
  17. Louloudes SJ, Vaughn JL and Dougherty KA (1973) Fatty acid profiles of cells from cell line IRPL-21 (Spodoptera frugiperda) and of the tissue culture medium after repeated use. In Vitro 8: 473–479.PubMedGoogle Scholar
  18. Lynn DE and Hink WF (1980) Comparison of nuclear polyhedrosis virus replication in five lepidopteran cell lines. Journal of Invertebrate Pathology 35: 234–240.CrossRefGoogle Scholar
  19. Mathews CK and Holde VEK (1990) Biochemistry. The Benjamin/Cummings Publishings, California.Google Scholar
  20. McIntosh AH and Grasela JJ (1994) Expression of b-galactosidase and luciferase in insect cell lines infected with a recombinant AcMNPV. In Vitro Cell Development Biology 30A: 275–278.Google Scholar
  21. Mitsuhashi J (1976) Insect cell line: amino acid utilization and requirements. In: Kurstak E and Maramorosch K (eds) Invertebrate tissue culture. (pp. 257–262) Academic Press, London.Google Scholar
  22. Mitsuhashi J, Makasone S and Hori Y (1985) Total fatty acids of some insect cell lines. App. Ent. Zool. 20: 8–12.Google Scholar
  23. Murhammer DW (1991) The use of insect cell cultures for recombinant protein sysnthesis: Engineering aspects. Applied Biochemistry and Biotechnology 31: 283–310.PubMedCrossRefGoogle Scholar
  24. O'Reilly DR, Miller LK and Luckow VA (1992) Baculovirus Expression Vectors: a laboratory manual. W. H. Freeman and Company, New York.Google Scholar
  25. Reuveny S, Kemp CW, Eppstein I and Shiloach J (1992) Carbohydrate metabolism in insect cell cultures during cell growth and recombinant protein production. In: Pederson H, Mytharasan R and Dibasio D (eds) Biochemical Engineering. Vol. 1.VII (pp. 230–237) New York Academy of Sciences.Google Scholar
  26. Reuveny S, Kim YJ, Kemp CW and Shiloach J (1993) Production of recombinant proteins in high-density insect cell cultures. Biotechnology and Bioengineering 42: 235–239.CrossRefPubMedGoogle Scholar
  27. Scott TRI, Blanchard JH and Ferguson CHR (1992) Effects of oxygen on recombinant protein production by suspension cultures of Spodoptera frugiperda (Sf9) insect cells. Enzyme Microbiology Technology 14: 798–804.CrossRefGoogle Scholar
  28. Stravroulakis DA, Kalogerakis N and Behie LA (1991a) Kinetic data for the BM-5 insect cell line in repeated-batch suspension culture. Biotechnology and Bioengineering 38: 116–126.CrossRefGoogle Scholar
  29. Stavroulakis DA, Kalogerakis N and Behie LA (1991b) Growth characteristics of a Bombyx mori Insect cell line in stationary and suspension culture. The Canadian Journal of Chemical Engineering 69: 457–464.CrossRefGoogle Scholar
  30. Stockdale H and Gardiner GR (1976) Utilization of some sugars by a line of Trichoplusia NI cells. In: Kurstak E and Maramorosch K (eds) Invertebrate Tissue Culture. (pp. 267–274) Academic Press, London.Google Scholar
  31. Stouthamer AH (1979) The search for correlation between theoretical and experimental yields. In: Ouayle JR (ed.) Microbial Biochemistry. University Park Press, Baltimore.Google Scholar
  32. Stouthamer AH, Bulthuis BA and Verseveld HWV (1990) Energetics of growth at low growth rates and its relevance for the maintenance concept. In: Poole RK et al. (eds) Microbial Growth and Dynamics. Oxford University Press.Google Scholar
  33. Taticek RA, Hammer DA and Shulker MA (1995) Overview of issues in bioreactor design and scale-up. In: Shuler ML et al. (eds) Baculovirus expression systems and biopesticides. (pp. 131–174) Wiley-Liss Inc., New York.Google Scholar
  34. Wang MY, Vakharia V and Bentley, WE (1993) Expression of epoxide hydrolase in insect cells: A focus on the infected cell. Biotechnology and Bioengineering 42: 240–246.CrossRefPubMedGoogle Scholar
  35. Wang MY, Kwong S and Bentley WE (1993) Effects of oxygen/glucose/glutamine feeding on insect cell baculovirus protein expression: A study on the Expoxide Hydrolase Production. Biotechnology Progress 9: 355–361.PubMedCrossRefGoogle Scholar
  36. Weiss SA, Gorfien A, Fike R, DiSorbo D and Jayme D (1990) Large scale production of proteins using serum-free insect cell culture. In: Ninth Australian Biotechnology Conference proceedings, Biotechnology: The science and business. (pp. 230–231) Gold Coast Australia.Google Scholar
  37. Wickham TJ, Davis T, Granados RR, Shuler ML and Wood HA (1992) Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system. Biotechnology Progress 8: 391–396.PubMedCrossRefGoogle Scholar
  38. Wickham TJ, Davis T, Granados RR, Hammer DA, Shuler ML and Wood HA (1991) Baculovirus defective interfering particles are responsible for variations in recombinant protein production as a function of multiplicity of infection. Biotechnology Letters 13: 483–488.CrossRefGoogle Scholar
  39. Wilkie GEI, Stockdale H and Prit SV (1980) Chemically defined media for production of insect cells and viruses in vitro. Develop. Biol. Standard. 46: 29–37.Google Scholar
  40. Wong TKK, Nielsen LK, Greenfield PF and Reid S (1994) Relationship between oxygen uptake rate and time of infection of Sf9 insect cells infected with a recombinant baculovirus. Cytotechnology 15: 157–167.PubMedCrossRefGoogle Scholar
  41. Wood AH (1995) Development and testing of genetically improved baculovirus insecticides. In: Shuler ML et al. (eds) Baculovirus expression system and biopesticides. (pp. 91–102) Wiley-Liss, New York.Google Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Ravinder Bhatia
    • 1
  • Gary Jesionowski
    • 1
  • Jerome Ferrance
    • 1
  • Mohammad M. Ataai
    • 1
  1. 1.Chemical Engineering and Center for Biotechnology and Bioengineering, 300 Technology DriveUniversity of PittsburghPittsburghU.S.A

Personalised recommendations