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Effects of Auxins and 2,3,5-Triiodobenzoic Acid on Somatic Embryo Initiation from Norway Spruce Zygotic Embryos (Picea abies)

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Abstract

Embryonal-suspensor mass (ESM) initiation from zygotic embryo sections was not affected by explant orientation whatever the type of external auxin used. Thus, polar auxin transport might not be directly involved in the control of somatic embryo formation. Application of 40 µM 2,3,5-triiodobenzoic acid (TIBA) suppressed ESM initiation in hypocotyl sections. This effect of TIBA mimicked that of a supraoptimal dose of α-naphtaleneacetic acid suggesting a detrimental effect of fast internal auxin accumulation on ESM initiation.

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Authors and Affiliations

  1. Research Centre for Plant Growth and Development, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, South Africa

    A.V. Ramarosandratana & J. Van Staden

Authors
  1. A.V. Ramarosandratana
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  2. J. Van Staden
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Ramarosandratana, A., Van Staden, J. Effects of Auxins and 2,3,5-Triiodobenzoic Acid on Somatic Embryo Initiation from Norway Spruce Zygotic Embryos (Picea abies). Plant Cell, Tissue and Organ Culture 79, 105–107 (2004). https://doi.org/10.1023/B:TICU.0000049446.77837.d7

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  • DOI: https://doi.org/10.1023/B:TICU.0000049446.77837.d7

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