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Phosphatase Inhibitors and Premature Chromosome Condensation in Human Peripheral Lymphocytes at Different Cell-Cycle Phases

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Somatic Cell and Molecular Genetics

Abstract

The cytogenetical reaction of human peripheral lymphocytes to okadaic acid and calyculin A was examined. Calyculin A could induce PCC about 20 times more effectively than okadaic acid. Their mechanisms of PCC induction were judged similar by their dose-dependent manner and chromosome morphology. Contrary to earlier studies suggesting that chemicals could not induce PCC in G 1 cells where little cyclin B is present, the present study showed that calyculin A could induce PCC in lymphocytes not only at S and G 2 /M but also at the second G 1 phase after PHA stimulation in vitro. PCC was induced slightly in lymphocytes both at G 0 and the first in vitro G 1 phase even when the calyculin A concentration increased one hundred fold. It was found that calcium ionophore A23187 increased frequencies of G 0 -PCC induced by calyculin A, although a further refinement is necessary to obtain a suitable morphology of G 0 -PCC for cytogenetic studies.

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Authors and Affiliations

  1. Division of Radiobiology and Biodosimetry, National Institute of Radiological Sciences, 4–9–1, Anagawa, Inage-ku, Chiba, 263–8555, Japan.

    Reiko Kanda, Kiyomi Eguchi-Kasai & Isamu Hayata

Authors
  1. Reiko Kanda
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  2. Kiyomi Eguchi-Kasai
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  3. Isamu Hayata
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Correspondence to Reiko Kanda.

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Kanda, R., Eguchi-Kasai, K. & Hayata, I. Phosphatase Inhibitors and Premature Chromosome Condensation in Human Peripheral Lymphocytes at Different Cell-Cycle Phases. Somat Cell Mol Genet 25, 1–8 (1999). https://doi.org/10.1023/B:SCAM.0000007135.12486.e3

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  • DOI: https://doi.org/10.1023/B:SCAM.0000007135.12486.e3

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