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PKB phosphorylation and survivin expression are cooperatively regulated by disruption of microfilament cytoskeleton

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Abstract

Changes in cell shape can lead to detachment and cell death, and the disruption in the actin cytoskeletal network, as one marker of cell shape changes, can itself induce apoptosis. In this study, the effects of cytochalasin B on the apoptosis-related proteins, protein kinase B and survivin were investigated. Apoptosis induced by disruption of microfilaments with cytochalasin B was found, although it happened at a low level, to simultaneously occur with G2/M arrest in 50% of the cytochalasin B-treated cells. During apoptosis, PKB phosphorylation and survivin expression was decreased by cytochalasin B, and the decline in survivin expression were preceded by PKB dephosphorylation, which implicated that survivin may be a target of PKB protein. The G2/M arrest of cytochalasin B-treated cells may be the direct function of cytochalasin B to microfilaments or the subsequent inhibition of survivin expression, or both. These results suggest that PKB/survivin signaling pathway may be responsible for the apoptosis induced by the disruption of actin cytoskeleton.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, P.R. China

    Yu-Long Liang, Li-Ying Wang, Heng Wu, Dong-Zhu Ma & Zhen Xu

  2. Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai

    Xi-Liang Zha

  3. Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai, 200032, P.R. China

    Xi-Liang Zha

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  1. Yu-Long Liang
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Liang, YL., Wang, LY., Wu, H. et al. PKB phosphorylation and survivin expression are cooperatively regulated by disruption of microfilament cytoskeleton. Mol Cell Biochem 254, 257–263 (2003). https://doi.org/10.1023/A:1027385417793

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