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Regulation of phagocytic process of macrophages by noradrenaline and its end metabolite 4-hydroxy-3-metoxyphenyl-glycol. Role of α- and β- adrenoreceptors

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Abstract

The regulatory capacity of noradrenaline and its end metabolite 4-hydroxy-3-metoxyphenylglycol (HMPG) on the complete phagocytic process of macrophages were investigated. Either noradrenaline or HMPG did not modify adherence. However, 10−12 M of noradrenaline stimulated the chemotaxis of macrophages, mainly mediated by α-adrenergic receptors. In contrast, 10−12 M of HMPG induced an opposed effect on this stage of the phagocytic process. To stimulate phagocytosis, it is necessary to employ a higher concentration (10−5 M) of noradrenaline and this effect was blocked with either 10−6 M propranolol or 10−6 M phentolamine, and maintained by HMPG. Noradrenaline and HMPG did not modify the microbicide capacity of macrophages (measured by O2 − production after phagocytosis). In conclusion, noradrenaline modulates the phagocytic process of macrophages, and this modulation is completed by HMPG, maintaining the phagocytic functions at physiologically optimal levels. Modulation of chemotaxis is mainly mediated by a-receptors and phagocytosis needs both α- and β-receptor-stimulation.

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References

  1. Besedovsky HO, Del Rey A: Immune-neuro-endocrine interactions: Facts and hypotheses. Endocr Rev 17: 64-102, 1996

    Google Scholar 

  2. Madden KS, Sanders VM, Felten DL: Catecholamine influences and sympathetic neural modulation of immune responsiveness. Annu Rev Pharmacol Toxicol 35: 417-448, 1995

    Google Scholar 

  3. Abrass CK, O'Connor SW, Scarpace PJ, Abrass IB: Characterization of the β-adrenergic receptor of the rat peritoneal macrophage. J Immunol 135: 1338-1341, 1985

    Google Scholar 

  4. Koff WC, Dunegan MA: Modulation of macrophage-mediated tumoricidal activity by neuropeptides and neurohormones. J Immunol 135: 350-354, 1985

    Google Scholar 

  5. Koff WC, Dunegan MA: Neuroendocrine hormones suppress macrophage-mediated lysis of herpes simplex virus infected-cells. J Immunol 136: 705-709, 1986

    Google Scholar 

  6. Ortega E, Garcia JJ, De la Fuente M: Modulation of adherence and chemotaxis of macrophages by norepinephrine. Influence of ageing. Mol Cell Biochem 203: 113-117, 2000

    Google Scholar 

  7. Ortega E, Garcia JJ, Saez MC, De la Fuente M: Changes with ageing in the modulation of macrophages by norepinephrine. Mech Ageing Dev 188: 103-114, 2000

    Google Scholar 

  8. Saez MC, Garcia JJ, De la Fuente M, Ortega E: Modulation of superoxide anion levels of macrophages from young-adult and old mice by the norepinephrine metabolite, 4-hydroxy-3-methoxyphenyl-glycol. Exp Gerontol 37: 395-400, 2002

    Google Scholar 

  9. Sprengler RN, Chensue SW, Giacherio DA, Blenk N, Kunkel SL: Endogenous norepinephrine regulates tumor necrosis factor-α production from macrophages in vitro. J Immunol 152: 3024-3031, 1994

    Google Scholar 

  10. Carrasco M, Del Rio M, Hernanz A, De la Fuente M: Inhibition of human neutrophil function by sulfated and nonsulfated cholecystokinin octapeptides. Peptides 18: 415-422, 1997

    Google Scholar 

  11. Boyden SV: The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocyte. J Exp Med 115: 453-456, 1962

    Google Scholar 

  12. Del Rio M, De la Fuente M: Chemoattractant capacity of bombesin, gastrin-releasing peptide and neuromedin C is mediated through PKC activation in murine peritoneal leukocytes. Regul Pept 49: 185-193, 1994

    Google Scholar 

  13. Ortega E, Forner MA, Barriga C, De la Fuente M: Effect of age and of swimming-induced stress on the phagocytic capacity of peritoneal macrophages from mice. Mech Ageing Dev 70: 53-63, 1993

    Google Scholar 

  14. De la Fuente M, Campos M, Del Rio M, Hernanz A: Inhibition of murine peritoneal macrophage functions by sulfated cholecystokinin octapeptide. Regul Pept 155: 47-56, 1995

    Google Scholar 

  15. Jin GF, Guo YS, Smith ER, Houston CW: The effect of bombesin-related peptides on the phagocytic function of mouse phagocytes in vitro. Peptides 11: 393-396, 1990

    Google Scholar 

  16. De la Fuente M: Changes in the macrophages function with aging. Comp Biochem Physiol A 81: 935-938, 1985

    Google Scholar 

  17. Delgado M, Garrido E, De la Fuente M, Gomariz R: Pituitary adenylate cyclase activating polypeptide (PA CAP-38). Stimulates rat peritoneal macrophage functions. Peptides 17: 1097-1105, 1996

    Google Scholar 

  18. Madden KS, Livnat S: Catecholamine action and immunologic reactivity. In: R. Ader, DL. Felten, N. Cohen (eds). Psychoneuroimmunology, 2nd edn. Academic Press, San Diego, 1991, pp 283-310

    Google Scholar 

  19. Straub RH, Mayer M, Kreutz M, Leeb S, Scholmerich J, Falk W: Neurotransmitters of the sympathetic nerve terminal are powerful chemoattractants for monocytes. J Leukoc Biol 67: 553-558, 2000

    Google Scholar 

  20. Doherty DE, Haslett C, Tonnesen MG, Henson PM: Human monocyte adherence; a primary effect of chemotactic factors on the monocyte to stimulate adherence to human endothelium. J Immunol 138: 1762-1771, 1987

    Google Scholar 

  21. Forner MA, Collazos ME, Barriga C, De la Fuente M, Rodriguez AB, Ortega E: Effect of age on adherence and chemotaxis capacities of peritoneal macrophages. Influence of physical activity stress. Mech Ageing Dev 75: 179-189, 1994

    Google Scholar 

  22. Miles BA, Lafuse WP, Zwilling BS: Binding of α-adrenergic receptors stimulates the anti-mycobacterial activity of murine peritoneal macrophages. J Neuroimmunol 71: 19-24, 1996

    Google Scholar 

  23. Ali RA, Qureshi MA, McCorkle FM: Prolife of chicken macrophage function after exposure to catecholamine in vitro. Immunopharmacol Immunotoxicol 166: 11-25, 1994.

    Google Scholar 

  24. Hartmann G, Michna H, Schanzer W: Cardiac and suprarrenal mediators of peritoneal macrophage activity and training. J Sports Med 8: 157-162, 1987

    Google Scholar 

  25. Petermann H, Heymann S, Vogl S, Dargel K: Phagocytic function and metabolite production in tiocetamide-induced liver cirrhosis: A comparative study in perfused livers and cultured Kuppfer cells. J Hepatol 24: 468-477, 1996

    Google Scholar 

  26. Javierre MQ, Pinto LV, Lima AO, Sassine WA: Immunologic phagocytosis by macrophages: Effect by stimulation of alpha adrenergic receptors. Rev Bras Pesqui Med Biol 8: 271-274, 1975

    Google Scholar 

  27. Serio M, Potenza MA, Montagnani M, Mansi G, Mitolo-Chieppa D, Jirillo E: Beta-adrenoceptor responsiveness of splenic macrophages in normotensive and hypertensive rats. Immunopharmacol Immunotoxicol 18: 24-65, 1996

    Google Scholar 

  28. Narnaware YK, Baker BI: Evidence that cortisol may protect against the immediate effects of stress on circulating leukocytes in the trout. Gen Comp Endocrinol 103: 359-366, 1996

    Google Scholar 

  29. Silvestri M, Oddera S, Lantero S, Rossi GA: Beta 2-agonist-induced inhibition of neutrophil chemotaxis is not associated with modification of LFA-1 and Mac-1 expression or with impairment of polymorphonuclear leukocyte antibacterial activity. Respir Med 93: 416-423, 1999

    Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, University of Extremadura, 06071, Badajoz, Spain

    Juan José García, Maríia del Carmen Sáez & Eduardo Ortega

  2. Department of Animal Biology II, Complutense University, 27080, Madrid, Spain

    Mónica De la Fuente

Authors
  1. Juan José García
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  2. Maríia del Carmen Sáez
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  3. Mónica De la Fuente
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  4. Eduardo Ortega
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García, J.J., del Carmen Sáez, M., De la Fuente, M. et al. Regulation of phagocytic process of macrophages by noradrenaline and its end metabolite 4-hydroxy-3-metoxyphenyl-glycol. Role of α- and β- adrenoreceptors. Mol Cell Biochem 254, 299–304 (2003). https://doi.org/10.1023/A:1027345820519

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