Abstract
Effects of the superinfection with tobacco mosaic virus (TMV) on susceptible tobacco plants infected with potato virus Y (PVY) were determined. Dynamic changes in the TMV and/or PVY contents, the ribonucleases (RNases), the phosphomonoesterase (PME), the phosphodiesterase (PDE) and the glucose-6-phosphate dehydrogenase (G6P DH) activities were studied. The PVY infection caused a substantial reduction in the multiplication of TMV. The content of TMV in the PVY inoculated leaves amounts to 6 and 9 % in the PVY systemically infected leaves when compared with single TMV. Surprisingly, the challenging virus (TMV) enhanced the content of inducing virus (PVY) in the locally inoculated leaves up to 130 – 141 %. In contrast, the reduction of PVY content down to 35 – 40 % by TMV was seen in the PVY systemically infected leaves. The activities of the RNase, the PME, the PDE and the G6P DH were increased (when compared with the healthy plants) during the acute phase of single virus multiplication (PVY or TMV). The increase in the activities of the enzymes in the leaves with mixed infection was at least as high as the sum of the increases of single infections. Moreover, a higher increase than the sum was seen for G6P DH and PDE (by about 20 – 35 %).
Similar content being viewed by others
References
Aguilar, I., Sanchez, F., Ponz, F.: Different forms of interference between two tobamoviruses in two different hosts.-Plant Pathol. 49: 659-665, 2000.
Arnon, D.I.: Copper enzymes in isolated chloroplasts: polyphenol oxidase in Beta vulgaris.-Plant Physiol. 24: 1-5, 1949.
Bagi, G., Farkas, G.L.: On the nature of increase in ribonuclease activity in mechanically damaged tobacco leaf tissues.-Phytochemistry 6: 161-169, 1967.
Barker, H., Harrison, B.D.: Double infection, interference and superinfection in protoplasts exposed to two strains of raspberry ringspot virus.-J. gen. Virol. 40: 647-658, 1978.
Barna, B., Ibenthal, W.D., Heitefuss, R.: Extracellular RNase activity in healthy and rust infected wheat leaves.-Physiol. mol. Plant Pathol. 35: 151-160, 1989.
Bozart R.F.: Pea enation mosaic virus: glucose catabolism in leaves and pods of infected peas.-Phytopathology 59: 389-390, 1969.
Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976.
Chersi, A., Bernardi, A., Bernardi, G.: Studies on acid hydrolases.-Biochim. biophys. Acta 129: 12-22, 1966.
Clark, M.F., Adams, A.N.: Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses.-J. gen. Virol. 34: 473-483, 1977.
Diener, T.O.: Virus infection and other factors affecting ribonuclease activity of plant leaves.-Virology 14: 177-189, 1961.
Gooding, G.V., Hebert, T.T.: A simple technique for purification of tobacco mosaic virus in large quantities.-Phytopathology 57: 1285, 1967.
Goodman, R.M., Ross, A.F.: Independent assembly of virions in tobacco doubly infected by potato virus X and potato virus Y or tobacco mosaic virus.-Virology 59: 314-318, 1974.
Green, P.J.: The ribonucleases of higher plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 421-445, 1994.
Hammerschmidt, R.: Induced disease resistance: how do induced plants stop pathogens?-Physiol. mol. Plant Pathol. 55: 77-84, 1999.
Huth, W.: Das Verhalten einiger Enzyme des Kohlenhydratstoffwechsels in Kartoffel-X-Virus-kranken Tabakpflanzen.-Phytopathol. Z. 77: 117-124, 1973.
Kazmierczak, A., Knypl, J.S.: Endoribonuclease of Vicia faba ssp. minor radicles, and enhancement of its activity by chilling stress or abscisic acid.-Physiol. Plant. 91: 722-728, 1994.
Leiser, R., Richter, J.: Reinigung und einige Eigenschaften des Kartoffel-Y-Virus.-Arch. Phytopath. Pflanz.-Schutz 14: 337-350, 1978.
Mahmood, T., Rush, C.M.: Evidence of cross-protection between beet soilborne mosaic virus and beet necrotic yellow vein virus in sugar beet.-Plant Dis. 83: 521-526, 1999.
Mančal, P.: Methods of Enzyme Immunoassay.-ÚSOL, Prague 1987.
Nelson, R.S., Powell, P.A., Beachy, R.N.: Coat protein-mediated protection against virus infection.-In: Fraser, R.S.S. (ed.): Recognition and Response in Plant-Virus Interactions. Pp. 427-442. Springer-Verlag, Berlin-Heidelberg 1990.
Nhu, K.M., Mayee, C.D., Sarkar, S.: Reduced synthesis of PVY in PVX-infected tobacco plants.-Naturwissenschaften 69: 183-184, 1982.
Procházková, Z.: Interaction of cucumber mosaic virus and potato virus Y with tobacco mosaic virus.-Biol. Plant. 12: 297-304, 1970.
Randles, J.W.: Ribonuclease isozymes in Chinese cabbage systemically infected with turnip yellow mosaic virus.-Virology 36: 556-563, 1968.
Reddi, K.K.: Studies on the formation of tobacco mosaic virus ribonucleic acid: II. Degradation of host ribonucleic acid following infection.-Proc. nat. Acad. Sci. USA 50: 75-81, 1963.
Sequeira, L.: Cross protection and induced resistance: their potential for plant disease control.-Trends Biotechnol. 2: 25-29, 1984.
Šindelář, L.: Changes in the activity of glucose-6-phosphate dehydrogenase and some problems relating to its regulation in tobacco plants infected with potato virus Y.-Biol. Plant. 28: 440-448, 1986.
Šindelář, L., Šindelářová, M., Burketová, L.: Changes in activity of glucose-6-phosphate and 6-phosphogluconate dehydrogenase isozymes upon potato virus Y infection in tobacco leaf tissues and protoplasts.-Plant Physiol. Biochem. 37: 195-201, 1999a.
Šindelář, L., Šindelářová, M., Burketová, L.: Changes in glucose, fructose and saccharose metabolism in tobacco plants infected with potato virus Y.-Biol. Plant. 42: 431-439, 1999b.
Šindelářová, M., Šindelář, L.: Changes in composition of soluble intercellular proteins isolated from healthy and TMV infected Nicotiana tabacum L. cv. Xanthi-nc.-Biol. Plant. 44: 567-572, 2001.
Šindelářová, M., Šindelář, L., Burketová, L.: Dynamic changes in the activities of glucose-6-phosphate dehydrogenase, ribulose bisphosphate carboxylase and ribonuclease in tobacco leaves, leaf discs and mesophyll protoplasts in relation to TMV multiplication.-Physiol. mol. Plant Pathol. 51: 99-109, 1997.
Šindelářová, M., Šindelář, L., Burketová, L.: Influence of some auxin-like herbicides on TMV multiplication and PR-protein biosynthesis.-Biol. Plant. 43: 467-470, 2000a.
Šindelářová, M., Šindelář, L., Burketová, L.: Correlation between activity of ribonucleases and potato virus Y biosynthesis in tobacco plants.-Physiol. mol. Plant Pathol. 57: 191-199, 2000b.
Šindelářová, M., Šindelář, L., Burketová, L.: Glucose-6-phosphate dehydrogenase, ribonucleases and esterases upon tobacco mosaic virus infection and benzothiodiazole treatment in tobacco.-Biol. Plant. 45: 423-432, 2002.
Šindelářová, M., Šindelář, L., Burketová, L., Táborský, V., Kazda, J.: Potato virus-Y multiplication in susceptible tobacco cultivar and transgenic breeding line producing coat protein mRNA.-Biol. Plant. 41: 565-573, 1998.
Solymosy, F., Farkas, G.L.: Simultaneous activation of pentose phosphate shunt enzymes in a virus infected local lesion host plants.-Nature 195: 835, 1962.
Takahashi, T.: Dehydrogenase activities of the pentose phosphate pathway in tobacco leaf epidermis infected with tobacco mosaic virus.-Phytopathol. Z. 72: 29-33, 1971.
Urban, L.A., Sherwood, J.L., Rezende, J.A.M., Melcher, U.: Examination of mechanisms of cross protection with non-transgenic plants.-In: Fraser, R.S.S. (ed.): Recognition and Response in Plant-Virus Interactions. Pp. 415-426. Springer-Verlag, Berlin-Heidelberg 1990.
Valkonen, J.P.T.: Accumulation of potato virus Y is enhanced in Solanum brevidens also infected with tobacco mosaic virus or potato spindle tuber viroid.-Ann. appl. Biol. 121: 321-327, 1992.
Voinnet, O.: A viral movement protein prevents systemic spread of the gene silencing signal in Nicotiana benthamiana.-Cell 103: 157-167, 2000.
Watts, J.W., Dawson, J.R.O.: Double infection of tobacco protoplasts with brome mosaic virus and cowpea chlorotic mottle virus.-Virology 105: 501-507, 1980.
Wyen, H.V., Udvardy, J., Erdei, S., Farkas, G.L.: The level of a relatively purine-specific ribonuclease increases in virus-infected hypersensitive or mechanically injured tobacco leaves.-Virology 48: 337-341, 1972.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Šindelářová, M., Šindelář, L. Changes in Glucose-6-Phosphate Dehydrogenase, Ribonucleases, Esterases and Contents of Viruses in Potato Virus Y Infected Tobacco Superinfected with Tobacco Mosaic Virus. Biologia Plantarum 47, 99–104 (2003). https://doi.org/10.1023/A:1027341217317
Issue Date:
DOI: https://doi.org/10.1023/A:1027341217317