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The Role of Reactive Oxygen Species in Mitochondrial Permeability Transition

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Bioscience Reports

Abstract

We have provided evidence that mitochondrial membrane permeability transition induced by inorganic phosphate, uncouplers or prooxidants such as t-butyl hydroperoxide and diamide is caused by a Ca2+-stimulated production of reactive oxygen species (ROS) by the respiratory chain, at the level of the coenzyme Q. The ROS attack to membrane protein thiols produces cross-linkage reactions, that may open membrane pores upon Ca2+ binding. Studies with submitochondrial particles have demonstrated that the binding of Ca2+ to these particles (possibly to cardiolipin) induces lipid lateral phase separation detected by electron paramagnetic resonance experiments exploying stearic acids spin labels. This condition leads to a disorganization of respiratory chain components, favoring ROS production and consequent protein and lipid oxidation.

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Authors and Affiliations

  1. The Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP 13083-970, Brazil

    Anibal E. Vercesi, Alicia J. Kowaltowski, Mercedes T. Grijalba, André R. Meinicke & Roger F. Castilho

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  1. Anibal E. Vercesi
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  2. Alicia J. Kowaltowski
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  3. Mercedes T. Grijalba
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Vercesi, A.E., Kowaltowski, A.J., Grijalba, M.T. et al. The Role of Reactive Oxygen Species in Mitochondrial Permeability Transition. Biosci Rep 17, 43–52 (1997). https://doi.org/10.1023/A:1027335217774

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