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Changes in Activities of Antioxidant Enzymes in Sunflower Leaves of Different Ages

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Biologia Plantarum

Abstract

Role of superoxide dismutase isozymes and other antioxidant enzymes was studied in relation to leaf age in sunflower (Helianthus annuus L. cv. ACC 1508) at pre-flowering and grain filling stages. Relative water content (RWC) did not change much in leaves of different age and at the two stages. Protein content declined continuously from the youngest to the oldest leaf, while chlorophyll (Chl) and carotenoids (Car) contents increased down to 7th/9th leaf and declined in subsequent older leaves. Protein, Chl and Car contents were higher at pre-flowering than at seed filling stage. Superoxide dismutase (SOD), its isozymes, and ascorbate peroxidase (APO) and catalase (CAT) activities were highest in the 9th leaf and declined in subsequent older leaves. SOD and APO activities were higher at seed filling, except in oldest senescent (13th, 15th) leaves. Among SOD isozymes, Cu/Zn-SOD and Mn-SOD activities accounted for most of the total SOD, and only marginal activity was observed for Fe-SOD. Peroxidase activity increased from youngest to the oldest leaf at pre-flowering stage and down to 13th leaf at seed filling stage.

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References

  • Aharoni, N., Lieberman, M.: Patterns of ethylene production in senescing leaves.-Plant Physiol. 64: 796-800, 1979.

    PubMed  CAS  Google Scholar 

  • Anderson, M.D., Prasad, T.K. Stewart, C.R.: Changes in isozyme profiles of catalase, peroxidase and glutathione reductase during acclimation to chilling in mesocotyls of maize seedlings.-Plant Physiol. 109: 1247-1257, 1995.

    PubMed  CAS  Google Scholar 

  • Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949.

    PubMed  CAS  Google Scholar 

  • Asada, K.: Ascorbate peroxidase —a hydrogen peroxide scavenging enzyme in plants.-Physiol. Plant. 55: 235-241, 1992.

    Article  Google Scholar 

  • Bowler, C., Van Montague, M. Inze, D.: Superoxide dismutase and stress tolerance.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 83-116, 1992.

    Article  CAS  Google Scholar 

  • Bueno, P., Del Rio, L.A.: Purification and properties of glyoxysomal cuperozinc superoxide dismutase from watermelon (Citrullus vulgaris Scrad.).-Plant Physiol. 98: 331-336, 1992.

    Article  PubMed  CAS  Google Scholar 

  • Castillo, F.I., Penel, I., Greppin, H.: Peroxidase release induced by ozone in Sedum album leaves.-Plant Physiol. 74: 846-851, 1984.

    PubMed  CAS  Google Scholar 

  • Comba, M.E., Benavides, M.P., Tomaro, M.L.: Effect of salt stress on antioxidant defence system in soybean root nodules.-Aust. J. Plant Physiol. 25: 665-671, 1998.

    CAS  Google Scholar 

  • Del Rio, L.A., Sandalio, L.M., Palma, J.M., Bueno, P., Corpas, F.J.: Metabolism of oxygen radicals in peroxisomes and cellular implications.-Free Radical Biol. Med. 13: 557-580, 1992.

    Article  CAS  Google Scholar 

  • Del Rio L.A., Pastori, G.M., Palma, J.M., Sandalio, L.M., Sevilla, F., Corpas, F.J., Jimenez, A., Lopez-Huertas, E., Hernandez, J.A.: The activated oxygen role of peroxisomes in senescence.-Plant Physiol. 116: 1195-1200, 1998.

    Article  PubMed  CAS  Google Scholar 

  • Dhindsa, R.A., Plumb-Dhindsa, P., Thorpe, T.A.: Leaf senescence: Correlated with increased permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase.-J. exp. Bot. 126: 93-101, 1981.

    Google Scholar 

  • Dunaeva, S.E., Galeeva, A.Z.: [Changes in some photosynthetic characteristics of the flag leaf of wheat in the process of its senescence.]-Tr. prikl. Bot. Genet. Selek. 67: 30-37, 1980. [In Russ.]

    Google Scholar 

  • Doulis, A., Debian, N., Kingston-Smith, A.H., Foyer, C.H.: Characterization of chilling sensitivity in maize. I. Differential localization of antioxidants in maize leaves.-Plant Physiol. 114: 1031-1037, 1997.

    PubMed  CAS  Google Scholar 

  • Foyer, C.H.: Ascorbic acid.-In: Alscher, R.C., Hess, J.L. (ed.): Antioxidants in Higher Plants. Pp. 31-58. CRC Press, Boca Raton 1993.

    Google Scholar 

  • Foyer, C.H., Descourvieres, P., Kunert, K.J.: Protection against oxygen radicals: an important defence mechanism studied in transgenic plants.-Plant Cell Environ. 17: 507-523, 1994.

    Article  CAS  Google Scholar 

  • Fukushima, T., Murayama, H., Katoh, C.: Effect of bolting on post-harvest chlorophyll degradation of Japanese radishes.-J. jap. Soc. hort. Sci. 61: 933-939, 1993.

    Article  CAS  Google Scholar 

  • Greppin, H., Penel, C., Gaspar, T. (ed.).: Molecular and Physiological Aspects of Plant Peroxidases.-Geneva University, Geneva 1986.

    Google Scholar 

  • Gomez, J.M., Hernandez, J.A., Jimenez, A., Del Rio, L.A., Sevilla, F.: Differential response of antioxidant enzymes of chloroplasts and mitochondria to long term NaCl stress of pea plants.-Free Radical Res. 31 (Suppl.): 11-18, 1999.

    Google Scholar 

  • Hernandez, J.A., Campillo, A., Jimenez, A., Alarcon, J.J. Sevilla, F.: Response of antioxidant systems and leaf water relations to NaCl stress in pea.-New Phytol. 141: 241-251, 1999.

    Article  CAS  Google Scholar 

  • Hernandez, J.A., Corpas, F.J., Gomez, M., Del Rio, L.A., Sevilla, F.: Salt induced oxidative stress mediated by activated oxygen species in pea leaf mitochondria.-Physiol Plant. 89: 103-110, 1993.

    Article  CAS  Google Scholar 

  • Hernandez, J.A., Del Rio, L.A., Sevilla, F.: Salt stress induced changes in superoxide dismutase isozyme in leaves and mesophyll protoplast from Vigna unguiculata (L) Walp.-New Phytol. 126: 37-44, 1994.

    Article  CAS  Google Scholar 

  • Hernandez, J.A., Jimenez, A., Mullineaux, P., Sevilla, F.: Tolerance of pea (Pisum sativum L.) to long term salt stress is associated with induction of antioxidant defences.-Plant Cell Environ. 23: 853-862, 2000.

    Article  CAS  Google Scholar 

  • Hernandez, J.A., Olmos, E., Corpas, F.J., Sevilla, F., Del Rio, L.A.: Salt induced oxidative stress in chloroplast of pea plants.-Plant Sci. 105: 151-167, 1995.

    Article  CAS  Google Scholar 

  • Hiscox, J.D., Israelstam, G.F.: A method for extraction of chlorophyll from leaf tissue without maceration.-Can. J. Bot. 57: 1332-1334, 1979.

    CAS  Google Scholar 

  • Hurng, W.P., Kao, C.H.: Lipid peroxidation and antioxidative enzymes in senescing tobacco leaves during post flooding.-Plant Sci. 96: 41-44, 1994.

    Article  CAS  Google Scholar 

  • Inanaga, S., Kumura, A.:. Studies on (dry) matter production of the rape plant (Brassica napus L.) 1. Changes with growth in rates of photosynthesis and respiration of rape population.-Proc. Crop Sci. Soc. Jap. 43: 261-266, 1974.

    Google Scholar 

  • Lichtenthaler, H.K., Wellburn, W.R.: Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents.-Biochem. Soc. Trans. 11: 591-592, 1983.

    CAS  Google Scholar 

  • Lowry, O.H., Rosenbrough, N.J., Farr, A.I., Randal, R.J.: Protein measurement with Folin-phenol reagent.-J. biol. Chem. 193: 265-275, 1951.

    PubMed  CAS  Google Scholar 

  • Menconi, M., Sgherri, C.L.M., Pinzino, C., Navari-Izzo, F.: Activated oxygen production and detoxification in wheat plants subjected to a water deficit programme.-J. exp. Bot. 46: 1123-1130, 1995.

    CAS  Google Scholar 

  • Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts.-Plant Cell Physiol. 22: 867-880, 1981.

    CAS  Google Scholar 

  • Olsson, M.: Alteration in lipid composition and antioxidative protection during senescence in drought stressed plants and non-drought stressed plants of Pisum sativum.-Plant Physiol. Biochem. 33: 547-553, 1995.

    CAS  Google Scholar 

  • Procházková, D., Sairam, R.K., Srivastava, G.C., Singh, D.V.: Oxidative stress and antioxidant activity as the basis of senescence in maize leaves.-Plant Sci. 161: 765-771, 2001.

    Article  Google Scholar 

  • Sairam, R.K., Srivastava, G.C.: Water stress tolerance of wheat (Triticum aestivum L.): Variations in hydrogen peroxide accumulation and antioxidant activity in tolerant and susceptible genotypes.-J. Agron. Crop Sci. 186: 63-70, 2001.

    Article  CAS  Google Scholar 

  • Sairam, R.K, Srivastava, G.C. Saxena, D.C.: Increased antioxidant activity under elevated temperature: a mechanism of heat stress tolerance in wheat genotypes.-Biol. Plant. 43: 245-251, 2000.

    Article  CAS  Google Scholar 

  • Sairam, R.K., Saxena, D.C.: Oxidative stress and antioxidants in wheat genotypes: Possible mechanism of water stress tolerance.-J. Agron. Crop Sci. 184: 55-61, 2000.

    Article  CAS  Google Scholar 

  • Sandalio, L.M., Palma, J.M., del Rio, L.A.: Localization of manganese-superoxide dismutase in peroxisomes isolated from Pisum sativum L.-Plant Sci. 51: 1-8, 1987.

    Article  CAS  Google Scholar 

  • Scandalios, J.G.: Oxygen stress and superoxide dismutase.-Plant Physiol. 101: 7-12, 1993.

    PubMed  CAS  Google Scholar 

  • Scebba, F., Sabastiani, L., Vitagliano, C.: Protective enzymes against activated oxygen species in wheat (Triticum aestivum L.) seedlings. Response to cold acclimation.-J. Plant Physiol. 155: 762-768, 1999.

    CAS  Google Scholar 

  • Sehmer, L., Dizengremel, P.: Contribution to subcellular localization of superoxide dismutase isoforms of spruce needles and oak leaves.-J. Plant Physiol. 153: 5-6, 1998.

    Google Scholar 

  • Teranishi, Y., Tanaka, A. Osumi, M., Fukui, S.: Catalase activity of hydrocarbon utilizing candida yeast.-Agr. biol. Chem. 38: 1213-1216, 1974.

    CAS  Google Scholar 

  • Thompson, J.E., Legge, R.L., Barber, R.L.: The role of free radicals in senescence and wounding.-New Phytol. 105: 317-334, 1987.

    Article  CAS  Google Scholar 

  • Upadhyaya, A., Davis, T.D., Larsen, M.H., Waker, R.H., Sankhla, N.: Uniconazole-induced thermo-tolerance in soybean seedling root tissues.-Physiol. Plant. 79: 78-84, 1990.

    Article  CAS  Google Scholar 

  • Whetherley, P.E.: Studies in the water relations of cotton plants. I. The field measurement of water deficit in leaves.-New Phytol. 49: 81-87, 1950.

    Article  Google Scholar 

  • Ye, Z., Rodriguez, R., Tran, A., Hoang, H., de los Santos, D., Brown, S., Vellanoweth, R.L.: The developmental transition to flowering repress ascorbate peroxidase activity and induces enzymatic lipid peroxidation in leaf tissue in Arabidopsis thaliana.-Plant Sci. 158: 115-127, 2000.

    Article  PubMed  CAS  Google Scholar 

  • Yu, Q., Rengel, Z.: Drought and salinity differentially influence activities of superoxide dismutase in narrow-leafed lupines.-Plant Sci. 142: 1-11, 1999.

    Article  CAS  Google Scholar 

  • Zhang, J., Kirkham, M.B.: Antioxidant responses to drought in sunflower and sorghum seedlings.-New Phytol. 132: 361-373, 1996.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110012, India

    R.K. Sairam, D.V. Singh & G.C. Srivastava

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  1. R.K. Sairam
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  2. D.V. Singh
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  3. G.C. Srivastava
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Sairam, R., Singh, D. & Srivastava, G. Changes in Activities of Antioxidant Enzymes in Sunflower Leaves of Different Ages. Biologia Plantarum 47, 61–66 (2003). https://doi.org/10.1023/A:1027328814591

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