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Somatic Embryo Formation on Mature Abies alba × Abies cephalonica Zygotic Embryo Explants

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Biologia Plantarum

Abstract

Somatic embryogenesis was achieved from mature embryos excised from stored hybrid Abies alba × Abies cephalonica seeds. Embryogenic tissue formation occurred on SH medium supplemented with 1 mg dm−3 benzyladenine. The formation of embryogenic tissue was influenced by the time of storage of seeds. Initiation frequencies 27.2 – 29.0 % were obtained in embryos isolated from 6 month and 1 year stored seeds. Embryos excised from 4-year stored seeds showed no response. Embryogenic structures appeared on the surface of hypocotyl. They originated without previous callus formation. Embryogenic tissues were maintained in long-term cultures. After maturation treatment cotyledonary somatic embryos developed and germinated in small plantlets.

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  1. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, P. O. Box 39 A, SK-95007, Nitra 1, Slovak Republic

    T. Salaj & J. Salaj

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  1. T. Salaj
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  2. J. Salaj
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Salaj, T., Salaj, J. Somatic Embryo Formation on Mature Abies alba × Abies cephalonica Zygotic Embryo Explants. Biologia Plantarum 47, 7–11 (2003). https://doi.org/10.1023/A:1027312410957

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