Abstract
Many different oligosaccharides were produced by transferring the fructose residue of sucrose to maltose, cellobiose, lactose and sucrose (self-transfer), where their yields of fructosylated acceptor products accounted for 26–30% (w/w). The maximum conversion yield (30%) was obtained in fructosyl cellobioside formation with 500 g sucrose l−1 (substrate) and 200 g cellobiose l−1 (acceptor). These four acceptors gave various products having DP (degree of polymerization) 2–7 by successive transfer reactions.
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References
Heincke K, Demuth B, Jordening HJ, Buchholz K (1999) Kinetics of the dextransucrase acceptor reacton with maltoseexperimental results and modeling. Enzyme Microbiol. Technol. 24: 523–534.
Hirayama M, Sumi N, Hidaka H (1989) Purification and properties of a fructooligosaccharide-producing β-fructofuranosidase from Aspergillus niger ATCC 20611. Agric. Biol. Chem. 53: 667–673.
Jung KH, Yun JW, Kang KR, Lim JY, Lee JH (1989) Mathematical model for enzymatic production of fructo-oligosaccharides from sucrose. Enzyme Microbiol. Technol. 11: 491–494.
Kim BW, Choi JW, Yun JW (1998) Selective production of GF4-fructooligosaccharides from sucrose by a new transfructosylating enzyme. Biotechnol. Lett. 20: 1031–1034.
Kitaoka M, Robyt JF (1999) Mechanism of the action of Leuconostoc mesenteroides B-512 FMC dextransucrase: kinetics of the transfer of D-glucose to maltose and the effects of enzyme and substrate concentrations. Carbohydr. Res. 320: 183–191.
Lee CY, Yun CY, Yun JW, OH TK, Kim CJ (1997) Production of glucooligosaccharides by an acceptor reaction using two types of glucansucrase from Streptococcus sobrinus. Biotechnol. Lett. 19: 1227–1230.
Mayer RM, Matthews MM, Futerman CL, Parnaik VK, Jung SM (1981) Dextransucrase: acceptor substrate reactions. Arch. Biochem. Biophys. 208: 278–287.
Monchois V, Willemot R, Monsan P (1999) Glucanosucrase: mechanism of action and structure-fuction relationships. FEMS Microbiol. Rev. 23: 131–151.
Muramatsu M, Nakakuki T (1995) Enzymatic synthesis of novel fructosyl and oligofructosyl trehaloses by Aspergillus sydowi β-fructofuranosidase. Biosci. Biotechnol. Biochem. 59: 208–212.
Park JP, Bae JT, Yun JW (1999) Critical effect of ammonium ions on the enzymatic reaction of a novel transfructosylating enzyme for fructo-oligosaccharide production from sucrose. Biotechnol. Lett. 21: 491–494.
Park KH, Kim MJ, Lee HS, Han NS, Kim DM, Robyt JF (1998) Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors. Carbohydr. Res. 313: 235–246.
Pereira AM, Costa FAA, Rodrigues MI, Maugeri F (1998) In vitro synthesis of oligosaccharides by acceptor reaction of dextransucrase from Leuconostoc mesenteroides. Biotechnol. Lett. 20: 397–401.
Robyt JF, Kim D, Yu L (1995) Mechanism of dextran activation dextransucrase. Carbohydr. Res. 266: 293–299.
Yun JW (1996) Fructooligosaccharides-occurrence, preparation, and application. Enzyme Microbiol. Technol. 19: 107–117.
Yun JW, Song SK (1993) The production of high-content fructooligosaccharides from scurose by the mixed enzyme system of fructosyltransferase and glucose oxidase. Biotechnol. Lett. 15: 573–576.
Yun JW, Jung KH, Jeon YJ, Lee JH (1992) Continuous production of fructo-oligosaccharides by immobilized cells of Aureobasidium pullulans. J. Microbiol. Biotechnol. 2: 98–101.
Yun JW, Lee MG, Song SK (1994) Batch production of high-content fructo-oligosaccharides from sucrose by the mixed-enzyme system of β-fructofuranosidase and glucose oxidase. J. Ferment. Bioeng. 77: 159–163.
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Kim, Y.M., Park, J.P., Sinha, J. et al. Acceptor reactions of a novel transfructosylating enzyme from Bacillus sp.. Biotechnology Letters 23, 13–16 (2001). https://doi.org/10.1023/A:1026788224343
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DOI: https://doi.org/10.1023/A:1026788224343