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Supercritical CO2 extraction of velutinol A from Mandevilla velutina (Apocynaceae) cultured cells and MALDI-TOF MS analysis

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Abstract

MALDI-TOF MS analysis of supercritical CO2 extracted samples obtained from Mandevilla velutina cell cultures allowed the detection of the anti-bradykinin pregnanic steroid, velutinol A, using low amount of sample (1 g lyophilized cells), with minimum analyte isolation.

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Authors and Affiliations

  1. University Federal of Santa Catarina/CCA, Plant Morphogenesis/Biochemistry Laboratory, P.O. Box, 476, Florianopolis, Brazil

    M. Maraschin

  2. UFPR/Chemo-Biotechnology Biomass Lab., Curitiba, Brazil

    J.A. Sugui

  3. Purdue University, Biochemistry Department, West Lafayette, USA

    K.V. Wood

  4. Purdue University, Biochemistry Department, West Lafayette, USA

    C. Bonham

  5. USP/IQSC/Chromatography Lab., Sao Carlos, Brazil

    F.M. Lanças

  6. University Federal of Santa Catarina/CCA, Plant Morphogenesis/Biochemistry Laboratory, P.O. Box, 476, 88.049-900, Florianopolis, Brazil

    P.S. Araujo

  7. UFSC/Chemistry Department, Florianopolis, Brazil

    R.A. Yunes

  8. LACDR/Division of Pharmacognosy, Leiden University, Leiden, The Netherlands

    R. Verpoorte

  9. UFPR/Chemo-Biotechnology Biomass Lab., Curitiba, Brazil

    J.D. Fontana

Authors
  1. M. Maraschin
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  2. J.A. Sugui
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  3. K.V. Wood
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  4. C. Bonham
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  5. F.M. Lanças
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  6. P.S. Araujo
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  7. R.A. Yunes
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  8. R. Verpoorte
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  9. J.D. Fontana
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Maraschin, M., Sugui, J., Wood, K. et al. Supercritical CO2 extraction of velutinol A from Mandevilla velutina (Apocynaceae) cultured cells and MALDI-TOF MS analysis. Biotechnology Letters 23, 77–82 (2001). https://doi.org/10.1023/A:1026776815646

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  • DOI: https://doi.org/10.1023/A:1026776815646

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