Abstract
After a 6 min exposure of isolated thylakoids to 43 °C, the extent of phosphorylation of light-harvesting complex of photosystem 2 (LHC2) was higher than in control thylakoids kept at 25 °C. Similarly, the exposure of intact spinach plants to 43 °C in dark for 11 h induced higher extent of thylakoid LHC2 phosphorylation than in control plants kept at 25 °C. The induced ability of LHC2 for enhanced phosphorylation may enable better energy distribution in favour of photosystem 1.
This is a preview of subscription content, log in via an institution to check access.
References
Allen, J.F., Bennett, J., Steinback, K.E., Arntzen, C.J.: Chloroplast protein phosphorylation couples plastoquinone redox state to distribution of excitation energy between photosystems.-Nature 291: 25-29, 1981.
Bukhov, N.G., Mohanty, P.: Elevated temperature stress effects on photosystems: Characterization and evaluation of the nature of heat induced impairments.-In: Irrgang, K.D., Govindjee (ed.): Concept in Photobiology: Photosynthesis and Photomorphogenesis. Pp. 617-648. Narosa Publishing House, New Delhi 1999.
Ghanotakis, D.F., Yocum, C.F.: Photosystem II and oxygen evolving complex.-Annu. Rev. Plant Physiol. Plant. mol. Biol. 41: 255-276, 1990.
Gounaris, K., Brain, A.P.R., Quinn, P.J., Williams, W.P.: Structural reorganisation of chloroplast thylakoid membranes in response to heat stress.-Biochim. biophys. Acta 766: 198-208, 1984.
Joshi, M.K., Mohanty, P., van Rensen, J.J.S., Bose, S.: In situ prolonged partial inhibition of photosystem II in pea plants lead to an increase in the unit size and number of photosystem II units.-Plant Sci. 106: 19-30, 1995.
Joshi, M.K., Desai, T.S., Mohanty, P.: Temperature dependent alterations in the pattern of photochemical and non photochemical quenching associated in Photosystem II conditions of leaves.-Plant Cell Physiol. 16: 1221-1227, 1995.
Laemmli, U.K.: Cleavage of structural proteins during the assembly of head of bacteriophage T4.-Nature 227: 680-685, 1970.
Larsson, U.K., Jergil, B., Andersson, B.: Changes in the lateral distribution of the light-harvesting chlorophyll a/b-protein complex induced by its phosphorylation.-Eur. J. Biochem. 136: 25-29, 1983.
Larsson, U.K., Sundby, C., Andersson, B.: Characterization of two different subpopulations of spinach light-harvesting chlorophyll a/b-protein complex (LHC II): polypeptide composition, phosphorylation pattern and association with photosystem II.-Biochim. biophys. Acta 894: 59-68, 1987.
Nakatani, H.Y., Barber, J.: An improved method for isolating chloroplasts retaining their outer membrane.-Biochim. biophys. Acta 461: 510-512, 1977.
Porra, R.J., Thompson, W.A., Kriedemann, P.E.: Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophyll a and b extracted with four different solvents: Verification of the concentration of chlorophyll standard by atomic absorption spectroscopy.-Biochim. biophys. Acta 975: 384-394, 1989.
Staehelin, L.A., Arntzen, C.J.: Regulation of chloroplast membrane function: Protein phosphorylation changes the spatial organization of membrane components.-J. cell. Biol. 97: 1327-1337, 1983.
Whitmarsh, J., Govindjee: Photosynthesis.-In: Immergut, E.H. (ed.): Encyclopedia of Applied Physics. Vol. 13. Pp. 513-532. VCH Publishers, New York 1995.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Satpathy, M., Mohanty, P. Effects of High Temperature Exposure of Spinach Intact Plants and Isolated Thylakoids on Light-Harvesting Complex 2 Protein Phosphorylation. Biologia Plantarum 43, 433–435 (2000). https://doi.org/10.1023/A:1026775202073
Issue Date:
DOI: https://doi.org/10.1023/A:1026775202073