Abstract
Carrot cell suspension culture prior to immobilization by glutaraldehyde was permeabilised by Tween 80. The values of pH optimum for L-alanine aminopeptidase, L-proline iminopeptidase, and L-arginine aminopeptidase were 8.2, 7.4, and 7.9, respectively. The immobilized cells showed significantly lower aminopeptidase activity when compared to untreated cells. Alginate hydrogel was successfully used for immobilization of carrot cells retaining the activity of some aminopeptidases.
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Báleš, V., Gemeiner, P., Kuniak, L., Rexová-Benková, E., Vojtíšek, V., Zemek, L.: Enzýmové Inžinierstvo [Enzyme Engineering.]-Alfa, Bratislava 1987. [In Slovak.]
Benešová, M., Kovács, P., Senkpiel, K.: Aminopeptidase activity in poppy seedlings (Papaver somniferum L.)-Biochem. Physiol. Pflanz. 165: 173-175, 1974.
Berlin, J., Martin, B., Nowak, J., White, L., Wray, V., Ctrack, D.: Effect of permeabilization on the biotransformation of phenylalanine by immobilized tobacco cell cultures.-Z. Naturforsch. 44c: 249-254, 1989.
Blanáriková, V., Benešová, M., Šulková, A., Pšenák, M.: Callus culture of Chelidonium majus L.-Biológia (Bratislava) 51: 76-77, 1996.
Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein dye binding.-Anal. Biochem. 72: 248-254, 1976.
Brodelius, P., Deus, B., Mosbach, K., Zenk, M. H.: Immobilized plant cells for the production and transformation of natural products.-FEBS Lett. 103: 93-97, 1979.
Dixon, R. A.: Isolation and maintenance of callus and cell suspension cultures.-In: Dixon, R.A. (ed.): Plant Cell Culture. A Practical Approach. Pp. 1-20. IRL Press, Oxford-Washington 1991.
Doi, H., Kawakami, M.: Purification of aminopeptidase from Australian classified barley flour.-Int. J. Biochem. cell. Biol. 29: 345-352, 1997.
Furusaki, S., Nozawa, T., Isohara, T., Furuya, T.: Influence of substrate transport on the activity of immobilized Papaver somniferum cells.-Appl. Microbiol. Biotechnol. 29: 437-441, 1988.
Furuya, T., Yoshikawa, T., Taira, M.: Biotransformation codeinone to codeine by immobilized cells of Papaver somniferum L.-Phytochemistry 32: 999-1001, 1984.
Hansen, E.W., Olafsen, K., Klaveness, T.O., Kvernberg, P.O.: Probing the gelation of polyvinylalcohol-water-glutaraldehyde within a porous material by H n.m.r.-a preliminary investigation.-Polymer 39: 1279-1287, 1998.
Hasal, P., Vojtíšek, V., Čejková, A., Kleczek, P., Kofroňová, O.: An immobilized whole yeast cell biocatalyst for enzymatic sucrose hydrolysis.-Enzyme Microbiol. Technol. 14: 221-229, 1992.
Heymann, E., Erlansen-Albertsson, R., Mentlein, J., Naush, W., Sholz, G., Struckhoff, G., Yoshimoto, T.: Dipeptidylpeptidase IV (DPP IV, EC 3.4.14.5) as an enzyme of the plasma membrane.-Beitr. Wirkstofforsch. 38: 22-37, 1990.
Hulst, A.C., Tramper, J.: Immobilized plant cells. A literature survey.-Enzyme Microbiol. Technol. 11: 546-558, 1989.
Poór, J., Stano, J., Tintemann, H., Mičieta, K., Andriamainty, F., Klimecký, A.: Activity of β-D-galactosidase in immobilized cells of tomato.-Bull. Food Res. 37: 33-40, 1998a.
Poór, J., Stano, J., Čižmárik, J., Andriamainty, F., Borovkov, N.: Activity of α-D-galactosidase in immobilized cells of tomato.-Bull. Food Res. 37: 127-134, 1998b.
Radlowski, M., Kalinowski, A., Kralikowski, Z., Bartowiak, S.: Protease activity from maize pollen.-Phytochemistry 35: 24-32, 1994.
Shoichet, M.S., Li, R.H., White, M.L., Winn, S.R.: Stability of hydrogels used in cell encapsulation. An in vitro comparison of alginate and agarose.-Biotechnol. Bioeng. 50: 374-381, 1996.
Stano, J., Kovács, P., Pšenák, M., Barth, A., Beneš, K.: Aminopeptidases isolated from seedlings of Cucumis sativus L.-Biológia (Bratislava) 44: 321-328, 1989.
Stano, J., Kovács, P., Kákoniová, D., Lišková, D., Kirilova, N. V., Komov, V. P.: Activity of dipeptidyl peptidase IV in ginseng callus culture.-Biológia (Bratislava) 49: 353-357, 1994a.
Stano, J., Nemec, P., Kovács, P.: Biochemical and histochemical studies studies of dipeptidyl peptidase IV and proline iminopeptidase in gherkin seedlings.-Histochem. J. 26: 886-887, 1994b.
Stano, J., Nemec, P., Weissová, K., Kovács, P., Kákoniová, D., Lišková, D.: Decarboxylation of L-tyrosine and L-DOPA by immobilized cells of Papaver somniferum.-Phytochemistry 38: 859-860, 1995.
Stano, J., Bezáková, L., Kovács, P., Kákoniová, D., Lišková, D.: α-D-Galactosidase in immobilized plant cells.-Pharmazie 51: 245-247, 1996.
Stano, J., Bezáková, L., Kovács, P., Mičieta, K., Neubert, K., Menon, V.P., Andriamainty, F.H.: β-D-Galactosidase in immobilized cells of Panax ginseng (C.A.MEY).-Pharmazie 52: 569-570, 1997a.
Stano, J., Nemec, P., Bezáková, L., Kovács, P., Kákoniová, D., Neubert, K., Lišková, D.: Invertase in immobilized cells of Papaver somniferum L.-Pharmazie 52: 242-244, 1997b.
Stano, J., Nemec, P., Bezáková, L., Kákoniová, D., Kovács, P., Neubert, K., Lišková, D., Andriamainty, F., Mičieta, K.: β-D-Galactosidase in immobilized cells of gherkin-Cucumis sativus L.-Acta Biochim. Polon. 45: 621-625, 1998.
Trinder, P.: Determination of blood glucose using an oxide-peroxidase systems with a non-carcinogenic chromogen.-Ann. clin. Biochem. 6: 24-32, 1969.
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Hegedus, L., Vojtaššák, J., Bilisics, L. et al. Activity of Some Aminopeptidases in Immobilized Cells of Daucus carota. Biologia Plantarum 43, 463–466 (2000). https://doi.org/10.1023/A:1026756124363
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DOI: https://doi.org/10.1023/A:1026756124363