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Correlation of O2 uptake rate and mitochondrial activity in carrot cell cultures exposed to laminar fluid shear

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Abstract

Measuring uncoupled oxygen uptake rate (OUR) could provide a convenient method for quantifying changes in the metabolic activity of plant cultures caused by hydrodynamic shear. Experiments on Daucus carota (carrot) cells were performed in a novel O2-permeable Couette viscometer at varying levels of laminar shear (6 to 100 N m−2). When the uncoupled OUR of the cells was compared with mitochondrial activity (determined by 2,3,5-triphenyl tetrazolium chloride assay), a significant correlation was observed (R=0.91 by linear regression).

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Authors and Affiliations

  1. Department of Chemical Engineering, Adelaide University, Adelaide, SA, 5005, Australia

    Victor Wong, David Williams & Christopher Colby

  2. Department of Physiology, Adelaide University, Adelaide, SA, 5005, Australia

    David A. Saint

Authors
  1. Victor Wong
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  2. David Williams
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  3. Christopher Colby
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  4. David A. Saint
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Wong, V., Williams, D., Colby, C. et al. Correlation of O2 uptake rate and mitochondrial activity in carrot cell cultures exposed to laminar fluid shear. Biotechnology Letters 22, 1919–1924 (2000). https://doi.org/10.1023/A:1026752418909

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  • DOI: https://doi.org/10.1023/A:1026752418909

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