Abstract
The growth of the truffle, Tuber borchii, mycelial strain (1BO) was analysed using biomolecular methods. Total RNA evaluation and ergosterol assay revealed that transcriptional and metabolic activity reach a peak at around 30–50 days of growth and decrease by 70 days. mRNA electrophoretic profiles highlighted a very low degree of variation in gene expression during the growth of mycelium.
Similar content being viewed by others
References
Amicucci A, Rossi I, Potenza L, Zambonelli A, Agostini D, Palma F, Stocchi V (1996) Identification of ectomycorrhizae from Tuber species by RFLP analysis of the ITS regions. Biotechnol. Lett. 18: 821–826.
Amicucci A, Zambonelli A, Giomaro G, Potenza L, Stocchi V (1998) Identification of ectomycorrhizal fungi of the genus Tuber by species-specific ITS primers. Mol. Ecol. 7: 273–277.
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman SG, Smith SA, Struhl K (1992) Current Protocols in Molecular Biology. Toronto: Greene Publishing Associated.
Bertini L, Agostini D, Potenza L, Rossi I, Zeppa S, Zambonelli A, Stocchi V (1998) Molecular markers for the identification of the ectomycorrhizal fungus Tuber borchii. New Phytol. 139: 565–570.
Ekblad A, Wallander H, Näsholm T (1998) Chitin and ergosterol combined to measure total and living fungal biomass in ectomycorrhizas. New Phytol. 138: 143–149.
Liang P, Pardee AB (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257: 967–971.
Martin F, Deraruelle C, Hilbert JL (1990) An improved ergosterol assay to estimate fungal biomass in ectomycorrhizas. Mycol. Res. 94: 1059–1064.
Molina R (1979) Pure culture synthesis and host specificity of red adler mycorrhizae. Can. J. Bot. 57: 1223–1228.
Nylund J-E, Wallander H (1992) Ergosterol analysis as a means of quantifying mycorrhizal biomass. In: Norris JR, Read DJ, Varma AK, eds. Methods in Microbiology, Vol. 24. London: Academic Press, pp. 77–88.
Paolocci F, Rubini A, Granetti B, Arcioni S (1997) Typing Tuber melanosporum and chinese black species by molecular markers. FEMS Microbiol. Lett. 153: 255–260.
Pegler DN, Spooner BM, Young TWK (1993) British Truffles. A Revision of British Hypogeous Fungi. Kew: Royal Botanic Gardens.
Potenza L, Amicucci A, Rossi I, Palma F, De Bellis R, Cardoni P, Stocchi V (1994) Identification of Tuber magnatum Pico DNA markers by RAPD analysis. Biotechnol. Tech. 8: 93–98.
Salmanowicz WE, Nylund J-E (1988) High performance liquid chromatography determination of ergosterol as a measure of ectomycorrhizal infection in Scots pine. Eur. J. For. Path. 18: 291–298.
Saltarelli R, Ceccaroli P, Vallorani L, Zambonelli A, Citterio B, Malatesta M, Stocchi V (1998) Biochemical and morphological modifications during the growth of Tuber borchii mycelium. Mycol. Res. 102: 403–409.
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press.
Sisti D, Zambonelli A, Giomaro G, Rossi I, Ceccaroli P, Citterio B, Benedetti PA, Stocchi V (1998) In vitro mycorrhizal synthesis of micropropagated Tilia platyphyllos Scop. plantlets with Tuber borchii Vittad. mycelium in pure culture. Acta Horticulturae 457: 379–387.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zeppa, S., Potenza, L., Vallorani, L. et al. Evaluation of transcript level and biomass during mycelial development of the truffle, Tuber borchii. Biotechnology Letters 23, 17–20 (2001). https://doi.org/10.1023/A:1026744425252
Issue Date:
DOI: https://doi.org/10.1023/A:1026744425252