Abstract
Callus and suspension cultures were established from the leaves of Agastache rugosa. The suspension cell growth was maximum at 15 days after inoculation. The cellular content of rosmarinic acid increased slowly and reached maximum (0.42 mg g−1 dry wt) during the stationary phase of culture, after 18 days of inoculation. The addition of yeast extract preparation (MW < 10 000) at 50 μg ml−1 elevated the rosmarinic acid content up to 5.7-fold of that found in non-elicited suspension cells. The elicitation of yeast extract preparation was further 2-fold enhanced by the presence of benzothiadiazole, a synthetic activator of plant systemic acquired resistance, as compared to yeast extract alone. These results showed that benzothiadiazole can be used as a tool for enhancing secondary metabolite accumulation in cell cultures.
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References
Benhamou N, Belanger RR (1998) Benzothiadiazole-mediated induced resistance to Fusarium oxysporum f. sp. radicis-lycoperici in tomato. Plant Physiol. 118: 1203–1212.
Constabel CP, Ryan CA (1998) A survey of wound-and methyl jasmonate-induced leaf polyphenol oxidase in crop plants. Phytochemistry 47: 507–511.
Fesen MR, Kohn KW, Leteurtre F, Pommier Y (1993) Inhibitors of human immunodeficiency virus integrase. Proc. Natl. Acad. Sci. USA 90: 2399–2403.
Friedrich L, Lawton K, Ruess W, Masner P, Specker N, Rella MG, Meier B, Dincher S, Staub T, Uknes S, Metraux J-P, Kessmann H, Ryals J (1996) A benzothiazole derivative induces systemic acquired resistance in tobacco. Plant J. 10: 61–70.
Gertlowski C, Petersen M (1993) Influence of the carbon source on growth and rosmarinic acid production in suspension cultures of Coleus blumei. Plant Cell Tissue Organ Cult. 34: 183–190.
Gorlach J, Volrath S, Knauf-Beiter G, Hengy G, Beckhove U, Kogel KH, Ostendorp M, Staub T, Ward E, Kessmann H (1996) Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat. Plant Cell 8: 629–643.
Hippolyte I, Marin B, Baccou JC, Jonard R (1992) Growth and rosmarinic acid production in cell suspension cultures of Salvia officinalis L. Plant Cell Rep. 11: 109–112.
Ilieva M, Pavlov A (1997) Rosmarinic acid production by Lavandula vera MM cell-suspension culture. Appl. Microbiol. Biotechnol. 47: 683–688.
Katz VA, Thulke OU, Conrath U (1998) A benzothiazole primes parsley cells for augmented elicitation of defense responses. Plant Physiol. 117: 1333–1339.
Kim HK, Lee HK, Shin CG, Huh H (1999) HIV integrase inhibitory activity of Agastache rugosa. Arch. Pharm. Res. 22: 520–523.
Lawton KA, Friedrich L, Hunt M, Weymann K, Delaney T, Kessmann H, Staub T, Ryals J (1996) Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway. Plant J. 10: 71–82.
Lee CB (1980) Korean Flora. Seoul: Hyangmoonsa.
Lee HK, Oh SR, Kim JI, Kim J, Lee C (1995) Agastaquinone, a new cytotoxic diterpenoid quinone from Agastache rugosa. J. Nat. Prod. 58: 1718–1721.
López-Arnaldos T, López-Serrano M, Ros Barceló A, Calderón AA, Zapata JM (1994) Tentative evidence of a rosmarinic acid peroxidase in cell cultures from Lavandin (Lavandula × intermedia) flowers. Biochem. Mol. Biol. Int. 34: 809–816.
Mizukami H, Ellis BE (1991) Rosmarinic acid formation and differential expression of tyrosine aminotransferase isoforms in Anchusa officinalis cell suspension cultures. Plant Cell Rep. 10: 321–324.
Mizukami H, Ogawa T, Ohashi H, Ellis BE (1992) Induction of rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures by yeast extract. Plant Cell Rep. 11: 480–483.
Mizukami H, Tabira Y, Ellis BE (1993) Methyl-jasmonate induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures. Plant Cell Rep. 12: 706–709.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497.
Oh SR, Jung KY, Lee HK (1996) In vitro anticomplementary activity of phenylpropanoids from Agastache rugosa. Kor. J. Pharmocogn. 27: 20–25.
Pearson DA, Frankel EN, Aeschbach R, German JB (1997) Inhibition of endothelial cell-mediated oxidation of low-density lipoprotein by rosmary and plant phenolics. J. Agric. Food Chem. 45: 578–582.
Petersen M, Hausler E, Meinhard J, Karwatzki BM, Gerlowski C (1994) The biosynthesis of rosmarinic acid in suspension cultures of Coleus blumei. Plant Cell Tissue Organ Cult. 38: 171–179.
Sumaryono W, Proksch P, Hartmann T, Nimtzs M, Wray V (1991) Induction of rosmarinic acid accumulation in cell suspension cultures of Orthosiphon aristatus after treatment with yeast extract. Phytochemisrty 30: 3267–3271.
Szabo E, Thelen A, Petersen M (1999) Fungal elicitor preparations and methyljasmonate enhance rosmarinic acid accumulation in suspension cultures of Coleus blumei. Plant Cell Rep. 18: 485–489.
Yoon HJ, Kim HK, Ma C-J, Huh H (2000) Induced accumulation of triterpenoids in Scutellaria baicalensis suspension cultures using a yeast elicitor. Biotechnol. Lett. 22: 1071–1075.
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Kim, H.K., Oh, SR., Lee, HK. et al. Benzothiadiazole enhances the elicitation of rosmarinic acid production in a suspension culture of Agastache rugosa O. Kuntze. Biotechnology Letters 23, 55–60 (2001). https://doi.org/10.1023/A:1026738409671
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DOI: https://doi.org/10.1023/A:1026738409671