Abstract
The effect of lead and cadmium on biomass accumulation of cucumber seedlings (Cucumis sativus L.) as well as the contents of abscisic acid (ABA), free proline and soluble proteins in leaves were studied. Seedlings were subjected to lead nitrate or cadmium bromide in low concentrations (1 – 5 µM) for 1, 4 or 7 d, and then to the action of the same substances in high concentrations (500 – 1000 µM). The pretreatments of the seedlings with heavy metals in low concentrations enabled them to tolerate the subsequent high concentrations of cadmium and lead without injury. The plant responses to heavy metal treatment were accompanied by the accumulation of ABA, free proline and soluble proteins in leaf tissues.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ali, G., Srivastava, P.S., Iqbal, M.: Morphogenetic response and proline content in Bacopa monniera cultures grown under copper stress.-Plant Sci. 138: 191-195, 1998.
Bates, L.S., Waldren, R.P., Tear, G.D.: Rapid determination of free proline for water stress studies.-Plant Soil 39: 205-207, 1973.
Bierkens, J., Maes, J., Platse, F.V.: Dose-dependent induction of heat shock protein 70 synthesis in Raphidocelis subcapitata following exposure to different classes of environmental pollutants.-Environ Pollut. 101: 91-97, 1998.
Bradford, M.M.: A rapid and sensitive method of the quantitation of microgram quantities of protein utilizing the principle of protein dye binding.-Anal. Biochem. 72: 248-254, 1976.
Brown, H., Martin, M.H.: Pretreatment effects of cadmium on the root growth of Holcus lanatus L.-New Phytol. 89: 621-629, 1981.
Das, P., Samantaray, S., Rout, G.P.: Studies on cadmium toxicity in plants: A review.-Environ. Pollut. 98: 29-36, 1997.
Didierjean, L., Frendo, P., Nasser, W., Genot, G., Marivet, J., Burkad, G.: Heavy metal-responsive genes in maize: identification and comparison of their expression upon various form of abiotic stress.-Planta 199: 1-8, 1996.
Foy, C.D., Chaney, R.L., White, M.C.: The physiology of metal toxicity in plants.-Annu. Rev. Plant Physiol. 29: 511-566, 1978.
Hollenbach, B., Schreiber, L., Hartung, W., Dietz, K.-J.: Cadmium leads to stimilated expression of the lipid transfer protein genes in barley: implications for the involvement of lipid transfer proteins in wax assembly.-Planta 203: 9-19, 1997.
Kägi, J.H.R.: Overview of metallothionein.-Methods Enzymol. 205: 613-626, 1991.
Kudoyarova, G.R., Veselov, S.U., Erkeev, M.I., Gyuli-Zade, V.S., Gimaeva, R.M., Zagidullin, N.V., Shakirova, F.M.: [Immunoenzyme test-system for indoleacetic acid determination in maize seeds with use of labeled antibodies.]-Fiziol. Rast. 33: 1221-1227, 1986. [In Russ.]
Lin, C.-Y., Chen, Y.-M., Key, J.L.: Solute leakage in soybean seedlings under various heat shock regimes.-Plant Cell Physiol. 26: 1493-1498, 1985.
Leung, J., Giraudat, J.: Abscisic acid signal transduction.-Annu. Rev. Plant Physiol. Plant mol. Biol. 49: 199-222. 1998.
Muñoz, F.J., Dopico, B., Labrador, E.: A cDNA encoding a proline-rich protein from Cicer arietinum. Changes in expression during development and abiotic stresses.-Physiol. Plant. 102: 582-590, 1998.
Murphy, A., Zhou, J., Goldsbrough, P.B., Taiz, L.: Purification and immunological identification of metallothioneins 1 and 2 from Arabidopsis thaliana.-Plant Physiol. 113: 1293-1301, 1997.
Nishizono, H., Kubota, K., Suzuki, S., Ishii, F.: Accumulation of heavy metals in cell walls of Polygonum cuspidatum roots from metalliferous habitat.-Plant Cell Physiol. 30: 595-598, 1989.
Rauser, W.E.: Phytochelatins.-Annu. Rev. Biochem. 59: 61-86, 1990.
Rauser, W.E.: Compartmental efflux analysis and removal of extracellular cadmium from roots.-Plant Physiol. 85: 62-65, 1987.
Sanità di Toppi, L., Gabrielli, R.: Response to cadmium in higher plants.-Environ. exp. Bot. 41: 105-130, 1999.
Schat, H., Sharma, S.S., Vooijs, R.: Heavy metal-induced accumulation of free proline in a metal-tolerant and nontolerant ecotype of Silene vulgaris.-Physiol. Plant. 101: 477-482, 1997.
Shah, K., Dubey, R.S.: Effect of cadmium on proline accumulation and ribonuclease activity in rice seedlings: role of proline as a possible enzyme protectant.-Biol. Plant. 40: 121-130, 1997.
Sobolev, A.S., Mel'nichuk, Yu.P., Kalinin, F.L.: [Adaptation of plant to inhibitory effect of cadmium.]-Fiziol. Biokhim. kul't. Rast. 14: 84-88, 1982. [In: Russ.]
Titov, A.F., Talanova, V.V., Boeva, N.P., Minaeva, S.V., Soldatov, S.E.: The effect of lead ions on the growth of wheat, barley, and cucumber seedlings.-Russ. J. Plant Physiol. 42: 457-462, 1995.
Younis, M.E., Abbas, M.A., Shukry, W.M.: Salinity and hormone interactions in affecting growth, transpiration and ionic relations of Phaseolus vulgaris.-Biol. Plant. 36: 83-89, 1994.
Zenk, M.H.: Heavy metal detoxification in higher plants-a review.-Gene 179: 21-30, 1996.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Talanova, V., Titov, A. & Boeva, N. Effect of Increasing Concentrations of Lead and Cadmium on Cucumber Seedlings. Biologia Plantarum 43, 441–444 (2000). https://doi.org/10.1023/A:1026735603890
Issue Date:
DOI: https://doi.org/10.1023/A:1026735603890