Abstract
A functionally active anti-hepatitis B surface antigen single-chain Fv antibody fragment (scFv) was expressed in seeds of transgenic tobacco plants using genetic constructs for expression in the vacuole or the apoplastic fluid. Antibody levels close to 0.2% of the total soluble protein were found. After storage of the transgenic tobacco seeds for one year and a half a year at room temperature, the scFv maintained its antigen-binding activity in full.
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Ayala M, Balint RF, Fernández de Cossio ME, Cannán-Haden L, Larrick JW, Gavilondo JV (1995) Variable region sequence modulates periplasmic export of a single chain Fv antibody fragment in E. coli. Biotechniques 18: 832–842.
Canaán-Haden L, Ayala M, Fernández-de-Cossio ME, Pedroso I, Rodés L, Gavilondo JV (1995) Purification and application of a single chain Fv antibody fragment specific to hepatitis B virus surface antigen. Biotechniques 19: 606–614.
Coego A, Vazquez R, Alfonso J, Coll Y, Pujol M, Menéndez E, López MA, Molina P, Hernández L, Bencomo B, De la Riva G, Selman G (1996) Effect of promoter-stimulatory element combination on transient reporter gene expression in tobacco protoplast using PEG-treatment. Biotecnol. Appl. 13: 147.
Fiedler U, Conrad U (1995) High-level production and long-term storage of engineered antibodies in transgenic tobacco seeds. Biotechnology 13: 1090–1093.
Kusnadi A, Hood E, Witcher D, Howard J, Nikolov Z (1998) Production and purification of two recombinant proteins from transgenic corn. Biotechnol. Prog. 14: 149–155.
Larrick J, Yu L, Hood Chen J, Jaiswal S, Wycoff K (1998) Production of antibodies in transgenic plants. Res. Immunol. 149: 603–608.
Parmenter D, Boothe J, van Rooijen G, Yeung E, Moloney M (1995) Production of biologically active hirudin in plant seeds using oleosin partitioning. Plant. Mol. Biol. 29: 1167–1180.
Phillips J, Fiedler U, Horstmann C, Mock H-P, Müntz K (1997) Seed-specific immunomodulation of abscisic acid activity induces a developmental switch. EMBO J. 16: 4489–4496.
Ramírez N, Ayala M, Lorenzo D, Palenzuela D, Herrera L, Doreste V, Falcón V, Gavilondo J, Oramas P (2000a) Expression of single-chain antibody fragments (scFv) against the hepatitis B antigen (HBsAg) in transgenic tobacco plants. Transgen. Res. (in press).
Ramírez N, Lorenzo D, Herrera L, Ayala M, Fuentes A, Pérez M, Gavilondo J, Oramas P (2000b) Single-chain antibody fragments specific to the hepatitis B surface antigen, produced in recombinant tobacco cell cultures. Biotechnol. Lett. 22: 1233–1236.
Zambrysky P, Joos H, Gentello J, Leemans J, Van Montagu M, Schell J (1983) Ti-plasmid vector for introduction of DNA into plant cells without altering their normal regeneration capacity. EMBO J. 2: 2143–2150.
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Ramírez, N., Oramas, P., Ayala, M. et al. Expression and long-term stability of a recombinant single-chain Fv antibody fragment in transgenic Nicotiana tabacum seeds. Biotechnology Letters 23, 47–49 (2001). https://doi.org/10.1023/A:1026728721966
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DOI: https://doi.org/10.1023/A:1026728721966