Abstract
Eight 6–9 month old calves, showing clinical signs of intermittent haemoglobinuria, even after treatment with an antipiroplasmal drug (4,4-diamidinodiazoaminobenzene diaceturate), were examined for oxidative damage to their erythrocytes and the presence of hemoprotozoa in blood smears. Four calves without signs of haemoglobinuria served as controls. The blood smears from three of the eight calves contained piroplasms for Theileria annulata. Irrespective of the presence of piroplasms in their blood smears, the calves with haemoglobinuria had significantly (p<0.01) lower haemoglobin concentrations (Hb) and packed cell volumes (PCV). The lipid peroxide level in the erythrocytes, but not in the plasma, of calves with red urine was significantly (p<0.05) higher than that for the controls. It is concluded that haemoglobinuria, irrespective of the presence of piroplasms in blood smears, is associated with oxidative stress to erythrocytes and peroxidation of polyunsaturated fatty acids of cell membrane.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Banarjee, P.K., Guha, C., Gupta, R., Potdar, R.R. and Dutta, A.K., 1986. Studies on the efficacy of some anti-theilerial drugs with special reference to halofuginone lactate, in clinical cases of theileriosis incrossbred cattle. Indian Veterinary Journal, 63, 237-241
Clemens, M.R. and Waller, H.D., 1987. Lipid peroxidation in erythrocytes. Chemistry and Physics of Lipids, 45, 251-268
Coles, E.H., 1980.Veterinary Clinical Pathology, 3rd edn, (W.B. Saunders, Philadelphia)
Dwivedi, S.K., Muslih, N.J., Joshi, H.C. and Ahmed, N.J., 1984. Evaluation of chemotherapeutic agents against bovine theileriosis in Iraq. Indian Journal of Veterinary Medicine, 4, 37-40
Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R.J., 1952. Protein measurement with Folin phenol reagent. Journal of Biological Chemistry, 193, 265-275
Mishra, K.C., Achar, K.C. and Lama, N.D., 1983. Efficacy of some chemotherapeutic agents against clinical Theileria annulata infection in exotic cattle. Indian Veterinary Journal, 60, 313-318
Muduuli, D.S., Marquardt, R.R. and Guenter, W., 1982. Effect of dietary vicine and vitamin E supplementation on the productive performance of growing and laying chickens. British Journal of Nutrition, 47, 53-60
Murase, T., Ueda, T., Yamato, O., Tajima, M. and Maede, Y., 1996. Oxidative damage and enhanced erythrophagocytosis in canine erythrocytes infected with Babesia gibsoni. Journal of Veterinary Medical Science, 58, 259-261
Placer, Z.A., Cushman, L. and Johnson, B., 1966. Estimation of product of lipid peroxidation (malondialdehyde) in biochemical systems. Analytical Biochemistry, 16, 359-364
Radostits, O.M., Blood, D.C. and Gay, C.C., 1994. Veterinary Medicine, 8th edn, (W.B. Saunders, Philadelphia)
Snedecor, G.W. and Cochran, W.G., 1967. Statistical Methods, 6th edn, (Oxford and IBH, New Delhi), 258-268
Somashekaraiah, B.V., Padmaja, K. and Prasad, A.R., 1992. Effect of lead on lipid peroxidation of hepatic subcellular organelles of developing chick embryos. Biochemistry International, 27, 803-809
Urquhart, G.M., Armour, J., Duncan, J.C., Dunn, A.M. and Jennings, F.W., 1996. Veterinary Parasitology, 2nd edn, (Blackwell Science, Oxford), 246-249
Utley, H.G., Bernheim, F. and Hochsein, P., 1967. Effect of sulfhydryl reagents on peroxidation of microsomes. Archives of Biochemistry and Biophysics, 118, 29-32
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sahoo, A., Patra, R., Pathak, N. et al. Enhanced Lipid Peroxide Levels in the Erythrocytes of Calves with Haemoglobinuria. Vet Res Commun 25, 55–59 (2001). https://doi.org/10.1023/A:1026710002039
Issue Date:
DOI: https://doi.org/10.1023/A:1026710002039