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Enhancement of penicillin G hydrolysis using penicillin acylase to 6-aminopenicillanic acid by simultaneous chromatographic separation

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Abstract

Penicillin G (Pen-G) was hydrolyzed to 6-aminopenicillanic acid (6-APA) and phenylacetic acid (PAA) in a chromatographic reactor-separator using the mixture of immobilized Escherichia coli cells and a macroporous adsorbent as stationary phase and a phosphate buffer of pH 7.8 as eluant. Pen-G conversion of 98% was observed without adjustment of the eluant pH due to the effective separation of 6-APA from Pen-G and PAA. At a sample load of 600 mg Pen-G, the volume overload gave higher Pen-G conversion (86%) than the mass overload (68%), while their difference in product resolution (0.9 and 1.0, respectively) was insignificant.

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Authors and Affiliations

  1. Chemical Engineering Research Center, Tianjin University, Tianjin, 300072, P.R. China

    J.C. Wu, Z.M. He, Z.W. Han & K.T. Yu

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  1. J.C. Wu
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  2. Z.M. He
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  3. Z.W. Han
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  4. K.T. Yu
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Wu, J., He, Z., Han, Z. et al. Enhancement of penicillin G hydrolysis using penicillin acylase to 6-aminopenicillanic acid by simultaneous chromatographic separation. Biotechnology Letters 22, 1959–1962 (2000). https://doi.org/10.1023/A:1026706111815

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  • DOI: https://doi.org/10.1023/A:1026706111815

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