Abstract
Two genotypes of mungbean differing in the leaf shape, K-851 (trifoliate) and a mutant (pentafoliate), were exposed to salinity (0, 2.5, 5, 10 dS m−1) for 3, 6 and 9 d at flowering stage (40 – 45 d after sowing) to see the effect of salinity on nodule functioning. In both the genotypes, osmotic potential (ψs) and relative water content (RWC) of nodules decreased significantly, and proline content increased with increasing the salinity. The ψs of nodules was more decreased in the mutant than in genotype K-851, while reverse was true for RWC and proline accumulation. A remarkable increase in ethylene evolution was noticed from nodulated roots with the increasing level and duration of salinity and was much higher in K-851. A sharp decline in leghemoglobin content and acetylene reduction assay (ARA) of the nodules was observed with the salinity and this decrease was more marked in K-851 than the mutant. N content declined while Na+/K+ ratio and Cl− content increased significantly. The mutant maintained better N status but lower Na+/K+ ratio and Cl− content in nodules than K-851. Nodule dry matter also declined with salinity and the decrease was more expressed in K-851. Thus the functioning of nodules in the mutant was better than in the genotype K-851 under stress conditions.
References
Abd-Alla, M.H., Vuong, T.D., Harper, J.E.: Genotypic differences in dinitrogen fixation response to NaCl stress in intact and grafted soybean.-J. Crop Sci. 38: 72-78, 1998.
Abd El-Samad, H.M., Shaddad, M.A.K.: Salt tolerance of soybean cultivars.-Biol. Plant. 39: 263-269, 1997.
Ashraf, M., Waheed, A.: Responses of some genetically diverse lines of chickpea (Cicer arietinum L.) to salt.-Plant Soil 154: 257-266, 1993.
Bates, L.S., Waldren, R.P., Teare, L.D.: Rapid determination of free proline for water stress studies.-Plant Soil 39: 205-208, 1973.
Cordovilla, M.P., Ligero, F., Lluch, C.: Influence of host genotypes on growth, symbiotic performance and nitrogen assimilation in faba bean (Vicia faba L.) under salt stress.-Plant Soil 172: 289-297, 1995.
El-Sheikh, E.A.E., Wood, M:: Effect of salinity on growth, nodulation and nitrogen fixation and yield of chickpea (Cicer arietinum L.).-J. exp. Bot. 41: 1263-1269, 1990.
Fearn, J.C., La Rue, T.A.: Ethylene inhibitors restore nodulation of sym 5 mutants of Pisum sativum cv. Sparkle.-Plant Physiol. 96: 239-244, 1991.
Feng, J., Barker, A.V.: Ethylene evolution and ammonium accumulation by tomato plants with various nitrogen forms and regimes of acidity.-J. Plant Nutr. 15: 2457-2469, 1992.
Fernandez-Pascual, M., De Lorenzo, C., De Felipe, M.R., Rajalakshmi, S., Gordan, A.J., Thomas, B.J., Minchin, F.R.: The possible reasons for relative salt stress tolerance in nodules of white lupin cv. Multolupa.-J. exp. Bot. 47: 1709-1716, 1996.
Greenway, H., Munns, R.: Mechanism of salt tolerance in non-halophytes.-Annu. Rev. Plant Physiol. 31: 149-190, 1980.
Hafeez, F.Y., Aslam, Z., Malik, K.A.: Effect of salinity and inoculation on growth, nitrogen fixation and nutrient uptake of Vigna radiata (L.) Wilczek.-Plant Soil 106: 3-8, 1988.
Hamada, A.M., Khulaef, E.M.: Effects of salinity and heat-shock on wheat seedling growth and content of carbohydrates, protein and amino acids.-Biol. Plant. 37: 399-404, 1995.
Hardy, R.W.F., Holsten, R.D., Jackson, E.K., Burn, R.C.: The acetylene ethylene assay for N2-fixation: Laboratory and field evaluation.-Plant Physiol. 43: 1185-1207, 1968.
Hartree, E.F.: Haematin compounds.-In: Peach, K., Tracey, M.V. (ed.): Modern Methods of Plant Analysis. Pp. 197-245. Springer-Verlag, Berlin 1955.
Manchanda, H.R., Sharma, S.K., Mor, R.P.: Relative tolerance of pulses for chloride and sulphate salinity.-Indian J. agr. Sci. 61: 20-26, 1991.
Noble, C.L., Rogers, M.J.E.: Arguments for the use of physiological criteria for improving the salt tolerance in crops.-Plant Soil 146: 99-107, 1992.
Rawal, A., Nandwal, A.S., Kundu, B.S., Dayal, J., Sheokand, S.: Ethylene action inhibitor restores nodule initiation in Cicer arietinum L. in the presence of nitrate.-Indian J. Plant Physiol. 38: 247-249, 1995.
Serraj, R., Roy, G., Drevon, J.J.: Salt stress induces a decrease in the oxygen uptake of soybean nodules and in their permeability to oxygen diffusion.-Physiol. Plant. 91: 161-168, 1994.
Sharma, S.K.: Effect of salinity on uptake and distribution of Na+, Cl- and K+ in two wheat cultivars.-Biol. Plant. 38: 261-267, 1996.
Velagaleti, R.R., Marsh, S., Kramer, D., Fleischrman, D., Corbin, J.: Genotypic differences in growth and nitrogen fixation among soybean [Glycine max (L.) Merr.]cultivars grown under salt stress.-Trop. Agr. 67: 169-177, 1990.
Weatherley, P.E.: Studies on the water relations of the cotton plant I. The field measurement of water deficits in leaves.-New Phytol. 40: 81-97, 1950.
Zayed, M.A., Zeid, I.M.: Effect of water and salt stress on growth, chlorophyll, mineral ions and organic solutes contents and enzymes activity in mungbean seedlings.-Biol. Plant. 40: 351-356, 1998.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nandwal, A., Godara, M., Kamboj, D. et al. Nodule Functioning in Trifoliate and Pentafoliate Mungbean Genotypes as Influenced by Salinity. Biologia Plantarum 43, 459–462 (2000). https://doi.org/10.1023/A:1026704107525
Issue Date:
DOI: https://doi.org/10.1023/A:1026704107525