Abstract
A method for isolating a GTP-binding regulatory protein from starfish oocytes is described. The protein consists of three subunits with molecular weights of 40, 37, and about 8 kDa. It is shown that the 40-kDa subunit has a high GTPase activity and is susceptible to ADP-ribosylation by pertussis toxin. The latter property of this subunit proved to decrease upon its incubation with nonhydrolyzable GTP analogues. These data provide evidence that the plasma membrane of starfish oocytes contains a 40-kDa GTP-binding protein with properties characteristic of the alpha subunit of the inhibitory G i protein. The role of this protein in the transmembrane signal transmission from the 1-methyladenine receptor to intracellular effectors is discussed.
Similar content being viewed by others
REFERENCES
Chiba, K. andHoshi, M., G Protein Function in Starfish Oocyte Maturation, Invert. Rep. Dev., 1996, vol. 30, no. 1, pp. 117-122.
Gilman, A.G., G Proteins: Transducers of Receptor-Generated Signal, Annu. Rev. Biochem., 1987, vol. 56, pp. 615-649.
Goffenberg, S.I.,Panchenko, M.P.,Zhuravlev, I.V., andTkachuk, V.A., Identification and Purification of GTP-Binding Regulatory Proteins from Plasma Membranes of the Porcine Heart, Biokhimiya, 1990, vol. 55, no. 5, pp. 878-887.
Greenberg, C.S. andGaddock, P.R., Rapid Single-Step Membrane Protein Assay, Clin. Chem., 1982, vol. 28, no. 7, pp. 725-726.
Hoshi, M.,Chiba, K.,Matsumoto, M.,Tadenuma, H.,Takashi, K., andKatada, T., Pertussis Toxin-Sensitive G Protein Participating in Starfish Oocyte Maturation Induced by 1-Methyladenine, Invert. Rep. Dev., 1992, vol. 22, pp. 1-10.
Jaffe, L.A.,Gallo, C.J.,Lee, R.H.,Ho, Y.-K., andJones, T.L.Z., Oocyte Maturation in Starfish Is Mediated by the βλ-Subunit Complex of a G Protein, J. Cell Biol., 1993, vol. 121, pp. 775-783.
Karaseva, E.,Lamash, N., andKhotimchenko, Yu., 1-Methyladenine Inhibits Adenylate Cyclase in Starfish Oocytes, Invert. Rep. Dev., 1996, vol. 30, pp. 153-158.
Laemmli, U.K., Cleavage of Structural Proteins during of the Head of Bacteriophage T4, Nature, 1970, vol. 227, no. 5259, p. 680.
Lamash, N.,Karaseva, E.M., andKhotimchenko, Yu.S., Alteration of Starfish Oocyte Adenylate Cyclase Properties in Gametogenesis, Comp. Biochem. Physiol., 1993, vol. 105C, no. 3, pp. 531-534.
Lamash, N.E., Karaseva, E.M., and Khotimchenko, Yu.S., The Dynamics of Adenylate Cyclase Properties in the Course of Starfish Oocyte Growth, Ontogenez, 1994, vol. 25, no. 2, pp. 24-31.
Shilling, F.,Chiba, K., andHoshi, M., Pertussis Toxin Inhibits 1-Methyladenine-Induced Maturation in Starfish Oocytes, Dev. Biol., 1989, vol. 133, pp. 605-608.
Stryer, L. andBourne, H.R., G Proteins: A Family of Signal Transducers, Annu. Rev. Cell Biol., 1986, no. 2, pp. 391-419.
Tadenuma, H.,Takahashi, K.,Chiba, K.,Hoshi, M., andKatada, T., Properties of 1-Methyladenine Receptors in Starfish Oocyte Membranes: Involvement of Pertussis Toxin-Sensitive GTP-Binding Protein in the Receptor-Mediated Signal Transduction, Biochem. Biophys. Res. Commun., 1992, vol. 186, pp. 114-121.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lamash, N.E. Identification and Isolation of GTP-Binding Regulatory Protein from Starfish (Asterias amurensis) Oocyte Plasma Membranes. Biology Bulletin 28, 107–109 (2001). https://doi.org/10.1023/A:1026675124909
Issue Date:
DOI: https://doi.org/10.1023/A:1026675124909