Skip to main content
SpringerLink
Log in

Effects of Inflammatory Cytokines Induced by Helicobacter pylori Infection on Aminopyrine Accumulation in Parietal Cells Isolated from Guinea Pigs

  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

The effects of inflammatory cytokines induced byHelicobacter pylori infection on acid secretion have notbeen well defined. The purpose of this study was toinvestigate the direct effects of these cytokines on parietal cells isolated from guinea pigs. Weexamined the effects of human recombinant IL-1β(0.05-100 ng/ml), IL-8 (2-256 ng/ml), and TNF-α(0.625- 80 ng/ml) on acid secretion stimulated by three secretagogues (10-4 M histamine,10-4 M carbachol, and 10-5 Mtetragastrin) and on basal acid secretion from isolatedparietal cells, which was measured by the aminopyrineaccumulation method. None of three cytokines showed any significant effects onstimulated or basal acid secretion from isolated guineapig parietal cells. We concluded that inflammatorycytokines induced by Helicobacter pylori infection may affect acid secretion through mechanismsother than direct actions on parietal cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Marshall BJ: Virulence and pathogenicity of Helicobacter pylori. J Gastroenterol Hepatol 6:121–124, 1991

    Google Scholar 

  2. Dunn BE: Pathogenic mechanisms of Helicobacter pylori. Gastroenterol Clin North Am 22:43–57, 1993

    Google Scholar 

  3. Dixon MF: Pathophysiology of Helicobacter pylori infection. Scand J Gastroenterol 29( suppl 201):7–10, 1994

    Google Scholar 

  4. Ernst PB, Jin Y, Reyes VE, Crowe SE: The role of the local immune responsein the pathogenesis of peptic ulcer formation. Scand J Gastroenterol 29(suppl 205):22–28, 1994

    Google Scholar 

  5. Cave DR, William WK, Hoffman S: Production of two chemically distinct acid-inhibitory factors by Helicobacter pylori. Eur J Gastroenterol Hepatol 5:S23–S27, 1993

    Google Scholar 

  6. Hoffman JS, King WW, Fox JG, Janik D, Cave DR: Rabbit and ferret parietal cell inhibition by Helicobacter species. Dig Dis Sci 40:147–152, 1995

    Google Scholar 

  7. Beil W, Birkholz C, Wagner S, Sewing K-F: Interaction of Helicobacter pylori and its fatty acids with parietal cells and gastric H1 /K1-ATPase. Gut 35:1176–1180, 1994

    Google Scholar 

  8. Jablonowski H, Hengels KJ, Kramer N, Geis G, Opferkuch W, Strohmeyer G: Effects of Helicobacter pylori on dbc-AMP stimulated acid secretion by human parietal cells. Hepatogastroenterology 41:546–548, 1994

    Google Scholar 

  9. Moss SF, Legon S, Bishop AE, Polak JM, Calam J: Effects of Helicobacter pylori on gastric somatostatin in duodenal ulcer disease. Lancet 340:930–932, 1992

    Google Scholar 

  10. Queiroz DM, Mendes EN, Rocha GA, Moura SB, Resende LMH, Barbosa AJA, Coelho LGV, Passos MCE, Castro LP, Oliveira CA, Lima GF: Effects of Helicobacter pylori eradication on antral gastrin and somatostatin-immunoreactivecell density and gastrin and somatostatin concentrations. Scand J Gastroenterol 28:858–864, 1993

    Google Scholar 

  11. Sumii M, Sumii K, Tari A, Kawaguchi H, Yamamoto G, Takehara Y, Fukino Y, Kamiyasu T, Hamada M, Tsuda T, Yoshihara M, Haruma K, Kajiyama G: Expression of antral gastrin and somatostatin mRNA in Helicobacter pylori-infected subjects. Am J Gastroenterol 89:1515–1519, 1994

    Google Scholar 

  12. Uehara A, Okumura T, Sekiya C, Okamura K, Takasugi Y, Namiki M: Interleukin-1 inhibits the secretin of gastric acid in rats: Possible involvement of prostaglandin. Biochem Biophys Res Commun 163:1578–1584, 1989

    Google Scholar 

  13. Saperas E, Yang H, River C, Tache Y: Central action of recombinant interleukin-1 to inhibit acid secretion in rats. Gastroenterology 99:1599–1606, 1990

    Google Scholar 

  14. Saperas E, Cominelli F, Tache Y: Potent inhibition of gastric acid secretion by intravenous interleukin-1b and-1a in rats. Peptides 13:221–226, 1992

    Google Scholar 

  15. Wallace JL, Cucala M, Mugridge K, Parente L: Secretagogue-specific effects of interleukin-1 on gastric acid secretion. Am J Physiol 261:G559–G564, 1991

    Google Scholar 

  16. Weigert N, Schaffer K, Schusdjiarra V, Glassen M, Schepp W: Gastrin secretion from primary cultures of rabbit antral G cells: Stimulation by inflammatory cytokines. Gastroenterology 110:147–154, 1996

    Google Scholar 

  17. Tani N, Watanabe Y, Karasawa H, Hara M, Miwa T, Watanabe T, Watanabe K: Isolation of parietal cells from guinea pig gastric mucosa by a Percoll density gradient. Tokai J Exp Clin Med 9:395–401, 1984

    Google Scholar 

  18. Berglindh T, Helander HF, Obrink KJ: Effects of secretagogues on oxygen consumption, aminopyrine accumulation and morphology in isolated gastric glands. Acta Physiol Scand 97:401–414, 1976

    Google Scholar 

  19. Morris A, Nicholson G: Ingestion of Campylobacter pyloridis causes gastritis and raised fasting gastric pH. Am J Gastroenterol 82:192–199, 1987

    Google Scholar 

  20. Graham DY, Alpert LC, Smith JL, Yoshimura HH: Iatrogenic Campylobacter pylori infection is a cause of epidemic achlorhydria. Am J Gastroenterol 83:974–980, 1998

    Google Scholar 

  21. Sobala GM, Crabtree JE, Dixon MF, Schorah CJ, Taylor JD, Rathbone BJ, Heatley RV, Axon ATR: Acute Helicobacter pylori infection: clinical features, local and systemic immune response, gastric mucosal histology, and gastric juiceascorbic acid concentrations. Gut 32:1415–1418, 1991

    Google Scholar 

  22. Courillon-Mallet A, Launay J-M, Roucayrol A-M, Callebert J, Emond J-P, Tabuteau F, Cattan D: Helicobacter pylori infection: Physiopathologic implication of N a-Methyl histamine. Gastroenterology 108:959–966, 1995

    Google Scholar 

  23. Noach LA, Bosma NB, Jansen J, Hoek FJ, van Deventer SJH, Tytgat GNT: Mucosal tumor necrosis factor-a, interleukin-1b, and interleukin-8 production in patients with Helicobacter pylori infection. Scand J Gastroenterol 29:425–429, 1994

    Google Scholar 

  24. Moss SF, Legon S, Davies J, Calam J: Cytokine gene expression in Helicobacter pylori associated antral gastritis. Gutc 35:1567–1570, 1994

    Google Scholar 

  25. Robert A, Olafsson AS, Lancaster C, Zhang W: Interleukin-1 is cytoprotective, antisecretory, stimulates PGE2 synthesis by the stomach, and retards gastric emptying. Life Sci 48:123–134, 1991

    Google Scholar 

  26. Tache Y, Saperas E: Potent inhibition of gastric acid secretion and ulcer formation by centrally and peripherally administered interleukin 1. Ann NY Acad Sci 664:353–368, 1992

    Google Scholar 

  27. Esplugues JV, Barrachina MD, Calatayud S, Pique JM, Whittle BJR: Nitric oxidemediates then inhibition by interleukin-1b of pentagastrin-stimulated rat gastric acid secretion. Br J Pharmacol 108:9–10, 1993

    Google Scholar 

  28. Nompleggi DJ, Beinborn M, Roy A, Wolfe MM: The effect of recombinant cytokines on [14C]-aminopyrine accumulation by isolated canineparietal cells. J Pharmacol Exp Ther 270:440–445, 1994

    Google Scholar 

  29. Katsuura G, Gottschall PE, Dahl RR, Arimura A: Interleukin-1 beta increases prostaglandin E2 in the rat astrocyte cultures: Modulatory effect of neuropeptides. Endocrinology 124:3125–3107, 1989

    Google Scholar 

Download references

Authors
  1. Norio Tani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yoriko Watanabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takayoshi Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shgeru Muramatsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Masayuki Miyazawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Norio Kimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Takeshi Miwa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tani, N., Watanabe, Y., Suzuki, T. et al. Effects of Inflammatory Cytokines Induced by Helicobacter pylori Infection on Aminopyrine Accumulation in Parietal Cells Isolated from Guinea Pigs. Dig Dis Sci 44, 686–690 (1999). https://doi.org/10.1023/A:1026645321698

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1026645321698

Search

Navigation