Abstract
An impaired oxidation of butyrate has beensuggested as a causative factor of ulcerative colitisand, moreover, agents present in colonic luminalcontents impair butyrate oxidation in both rat and human colonocytes. To evaluate the overall effect offeces on the production of CO2 and ketonebodies from butyrate oxidation in rat colonocytes, fecalhomogenates from 10 healthy subjects and 10 patients with quiescent and 10 patients with activeulcerative colitis were sterile filtrated and added torat colonocytes incubated with 2, 4, and 10 mmol/literof stock butyrate, respectively. Addition of fecal filtrate from healthy subjects and patientswith quiescent and active ulcerative colitis tocolonocytes incubated with 2, 4, and 10 mmol/liter ofstock butyrate, respectively, tended to decrease theproduction of CO2 from butyrate oxidation,whereas ketogenesis was unaffected. The decrease inCO2 production was not explained by thesimultaneous addition of fecal short-chain fatty acids(SCFAs). However, a difference in the ability todecrease CO2 production was not found betweenfiltrates from healthy subjects and patients withquiescent and active ulcerative colitis. In conclusion, feces from healthy subjects and patients withquiescent and active ulcerative colitis containinhibitor(s) of the production of CO2 frombutyrate oxidation in colonocytes. However, a specific inhibitory effect of feces from patients withulcerative colitis on the production of CO2could not be identified.
Similar content being viewed by others
REFERENCES
Roediger WEW: Role of anaerobic bacteria in the metabolic welfare of the colonic mucosa in man. Gut 21:793–798, 1980
Roediger WEW: Utilization of nutrients by isolated epithelial cells of the rat colon. Gastroenterology 83:424–429, 1982
Roediger WEW: The colonic epithelium in ulcerative colitis- an energy deficiency disease? Lancet 2:712–715, 1980
Chapman MAS, Grahn MF, Boyle MA, Hutton M, Rogers J, Williams NS: Butyrate oxidation is impaired in the colonic mucosa of suffers of quiescent ulcerative colitis. Gut 35:73–76, 1994
Steinhart AH, Brzezinsky JA, Baker JP: Treatment of refractory ulcerative proctosigmoiditis with butyrate enemas. Am J Gastroenterol 89:179–183, 1994
Scheppach W, Sommer H, Kirchner T, Paganelli G-M, Bartram P, Christl S, Richter F, Dusel G, Kasper H: Effect of butyrate enemas on the colonic mucosa in distal ulcerative colitis. Gastroenterology 103:51–56, 1992
Senagore AJ, MacKeigan JM, Scheider M, Ebrom JS: Shortchain fatty acid enemas: a cost-effective alternative in the treatment of nonspecific proctosigmoiditis. Dis Colon Rectum 35:923–927, 1992
Harig JM, Soergel KH, Komorowsky RA, Wood CM: Treatment of diversion colitis with short-chain fatty acid irrigation. N Engl J Med 320:23–28, 1989
Breuer RI, Buto SK, Christ ML, Bean J, Vernia P, Paoluzi P, Di Paolo MC, Caprilli R: Rectal irrigation with short-chain fatty acids for distal ulcerative colitis: pre liminary report. Dig Dis Sci 36:185187, 1991
Finnie IA, Taylor BA, Rhodes JM: Ileal and colonic epithelial me tabolism in quiescent ulcerative colitis: increased glutamine me tabolism in distal colon but no defect in butyrate metabolism. Gut 34:1552–1558, 1993
Clausen MR, Mortensen PB: Kine tic studies on colonocyte me tabolism of short-chain fatty acids and glucose in ulcerative colitis. Gut 37:684–689, 1995
Steinhart AH, Hiruki T, Brzezinski A, Baker JP: Treatment of left-sided ulcerative colitis with butyrate enemas: A controlled trial. Aliment Pharmacol Ther 10:729–736, 1996
Scheppach W: Are short-chain fatty acids effective in the local treatment of ulcerative colitis? Gastroenterology 110:A1010, 1996
Breuer RI, Soergel KH, Lashner BA, Christ ML, Hanauer SB, Vanagunas A, Harig JM,-Keshavarzian A, Robinson M, Sellin JH, We inberg D, Vidican DE, Flemal KL, Rademaker AW: Short chain fatty acid rectal irrigation for left-sided ulcerative colitis: A randomized, placebo-controlled trial. Gut 40:485–491, 1997
Roediger WEW, Nance S: Selective reduction of fatty acid oxidation in colonocytes: Correlation with ulcerative colitis. Lipids 25:646–652, 1990
Roediger WEW, Duncan A, Kapaniris O, Millard S: Reducing sulfur compounds of the colon impair colonocyte nutrition: Implications for ulcerative colitis. Gastroenterology 104:802–809, 1993
Roediger WEW, Duncan A, Kapaniris O, Millard S: Sulphide impairment of substrate oxidation in rat colonocytes: A biochemical basis for ulcerative colitis? Clin Sci 85:623627, 1993
Darcy-Vrillon B, Cherbuy C, Morel M-T, Durand M, Duée P-H: Short chain fatty acid and glucose me tabolism in isolated pig colonocytes: modulation by NH4 +. Mol Cell Biochem 156:145151, 1996
Roediger WE, Radcliffe BC, Deakin EJ, Nance SH: Specific metabolic effect of sodiumnitrite on fat metabolism by mucosal cells of the colon. Dig Dis Sci 31:535–539, 1986
Hartman PE: Nitrates and nitrites: Ingestion, pharmacodynamics, and toxicology. In Chemical Mutagens: Principles and Methods for Their Detection, Vol 7. FJ De Serres, A Hollaender (eds). New York, Plenum Press, 1982, pp 211–294
Roediger WEW, Babidge W, Millard S: Methionine derivatives diminish sulphide damage to colonocytes-implications for ulcerative colitis. Gut 39:77–81, 1996
Roediger WEW, Truelove SC: Method of preparing isolated colonic epithelial cells (colonocytes) for metabolic studies. Gut 20:484–488, 1979
Krebs HA, Henseleit K: Untersuchungen über die harnstoffbildung im tierkörper. Hoppe-Seyler's Z Physiol Chem 210:33–66, 1932
Jørgensen JR, Clausen MR, Mortensen PB: Oxidation of short-and medium-chain C2-C8 fatty acids in Sprague-Dawley rat colonocytes. Gut 40:400–405, 1997
Kight CE, Fleming SE. Nutrient Oxidation by rat intestinal epithelial cells is concentration dependent. J Nutr 123:876–881, 1993
Gutmann I, Wahlefeld AW: Lactate determination with lactate dehydrogenase and NAD. In Methods of Enzymatic Analysis. HU Bergmeyer (ed). New York, Academic Press, 1974, pp 1464–1468
Zijlstra JB, Beukema J, Wolthers BG, Byrne BM, Groen A, Donkert L: Pretreatment methods prior to gas chromatographic analysis of volatile fatty acids from faecal samples. Clin Chim Acta 78:243–250, 1977
Cline JD: Spectrophotometric determination of hydrogen sul-de and reducible sulde in natural waters. Limnol Oceanogr 14:454–458, 1969
Florin THJ: Hydrogen sulphide and total acid-volatile sulphide in faeces, determined with a direct spectrophotometric method. Clin Chim Acta 196:127–134, 1991
Florin THJ, Gibson GR, Neale G, Cummings JH: A role for sulfate reducing bacteria in ulcerative colitis? Gastroenterology 98:A170, 1990
Gibson GR, Cummings JH, MacFarlane GT: Growth and activities of sulphate-reducing bacteria in gut contents of healthy subjects and patients with ulcerative colitis. FEMS Microbiol Ecol 86:103–112, 1991
Pitcher MCL, Beatty ER, Cummings JH: 5–aminosalicylic acid (5–ASA) inhibits luminal sul de production by gut bacteria in ulcerative colitis. Gastroenterology 110:A992, 1996
Hove H, Brøbech Mortensen P: Influence of intestinal inflammation (IBD) and small and large bowel length on fecal short-chain fatty acids and lactate. Dig Dis Sci 40:1372–1380, 1995
Clausen MR, Mortensen PB: Kinetic studies on the me tabolism of short-chain fatty acids and glucose by isolated rat colonocytes. Gastroenterology 106:423–432, 1994
Pitcher MCL, Beatty ER, Gibson GR, Cummings JH: Sulfatereducing bacteria: Prevalence in active and inactive ulcerative colitis. Gastroenterology 108:A894, 1995
Moore JWE, Roediger WEW, Babidge WJ: Colonic luminal hydrogen sulphide is not elevated in ulcerative colitis. Int J Colorectal Dis 11:151, 1996
Rights and permissions
About this article
Cite this article
Jorgensen, J.R., Mortensen, P.B. Influence of Feces from Patients with Ulcerative Colitis on Butyrate Oxidation in Rat Colonocytes. Dig Dis Sci 44, 2099–2109 (1999). https://doi.org/10.1023/A:1026642923168
Issue Date:
DOI: https://doi.org/10.1023/A:1026642923168