Abstract
Heterocyclic bases and phosphate groups involved in the DNA–methyltransferase SsoII (M · SsoII) interaction were identified in the regulatory DNA region localized within the promoter region of the SsoII restriction–modification genes by footprinting with the use of formic acid, hydrazine, dimethyl sulfate, and N-ethyl-N-nitrosourea as modifying agents. It has been established that the enzyme interacts with three guanines, one adenine, two thymines, and three phosphate groups of each strand of the DNA duplex. These heterocyclic bases and phosphate groups are disposed symmetrically within the 15-mer inverted repeat of the regulatory DNA region. It has been demonstrated by footprinting with dimethyl sulfate that the C7 atoms of guanines interacting with the enzyme are exposed to the DNA major groove. Two theoretical models were built describing the contacts in a complex between M · SsoII and the regulatory DNA region.
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Vorob'eva, O.V., Vasil'ev, S.A., Karyagina, A.S. et al. Analysis of DNA–Protein Contacts in a Complex between Methyltransferase SsoII and a Promoter Region of the SsoII Restriction–Modification Genes. Molecular Biology 34, 921–926 (2000). https://doi.org/10.1023/A:1026640212498
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DOI: https://doi.org/10.1023/A:1026640212498