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Morphological and Immunohistochemical Changes in Intestinal Mucosa and PYY Release Following Total Colectomy with Ileal Pouch-Anal Anastomosis in Dogs

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Abstract

Other studies have shown that both morphologicaland functional adaptation occur in the ileal mucosaafter total colectomy and may be mediated by humoralfactors. To elucidate the participation of peptide YY (PYY) in intestinal adaptation after totalproctocolectomy with ileal pouch-anal anastomosis(IPAA), changes in the number of PYY-containing cellsand in histological appearance in the intestinal mucosa, especially in the mucosa of ileal pouch, wereinvestigated in dogs. We further examined changes inpostprandial PYY release in relation to those inPYY-containing cells. Ten adult beagle dogs underwent IPAA. Before and 2, 6, and 12 months aftersurgery, a test meal was given, and blood samples weretaken from a foreleg vein at intervals for 3 hr formeasurements of plasma PYY concentration byradioimmunoassay. Before and one year after surgery,morphological studies of the intestinal mucosa wereperformed using parameters such as villous height (VH),mucosal thickness (MT), and villous index (VI).Immunohistochemical studies of PYY were also done in the intestinalmucosa. Both fasting and postprandial plasma PYY levelswere reduced to half of the preoperative levels at twomonths after surgery. Thereafter, postprandial levels approached preoperative concentrations,while fasting levels remained unchanged. VH, MT, and VIwere significantly smaller in the ileal pouch than inthe ileal end of the controls. The MT of the ileal pouch was similar to that of the colon.PYY-containing cells in the mucosa of the ileal pouchwere distributed more densely than those in the ilealend, similar to those in the colon of the controls. It was concluded that after IPAA, the pouchmucosa gradually changed to resemble the colonic mucosanot only in histological appearance but also in thepopulation of PYY-containing cells. It is therefore considered that colonic transformation of theilealpouch mucosa is closely related to the increase inthe number of PYY-containing cells and the steadyrecovery of postprandial PYY secretion.

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Authors
  1. Mikio Imamura
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  2. Hiroto Nakajima
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  3. Yukio Mikami
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  4. Hidemi Yamauchi
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Imamura, M., Nakajima, H., Mikami, Y. et al. Morphological and Immunohistochemical Changes in Intestinal Mucosa and PYY Release Following Total Colectomy with Ileal Pouch-Anal Anastomosis in Dogs. Dig Dis Sci 44, 1000–1007 (1999). https://doi.org/10.1023/A:1026620900259

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