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Role of Endothelin in Obstructive Jaundice

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Abstract

Mediators responsible for renal changes inobstructive jaundice are not specified. This study isdesigned to study the role of endothelin-1 (ET-1) inobstructive jaundice in rats. Animals were randomly placed into five experimental groups. Group 1(N = 3) was the sham-operated group. Group 2 (N = 8)after common bile duct (CBD) ligation, receivedbosentan, which is a nonselective endothelin receptorblocker, 50 mg/kg/day for seven days. Group 3 (N = 7)received 1 μg/kg/day captopril. Group 4 (N = 7) wasgiven both drugs orally for seven days. Group 5 (N = 6)after CBD ligation, received Arabic gum as the vehicle. Blood was drawn from the infrahepaticvena cava for the determination of ET-1, bilirubin,creatinine, protein oxidation products, hyaluronic acid,and β-N-acetyl-hexosaminase. Liver tissue samples were obtained to determine glutathionelevels. ET-1, protein oxidation products, hyaluronicacid, bilirubin, and creatinine levels increasedsignificantly in the control group when compared with sham. Bosentan effectively prevented ET-1elevation but could not reverse creatinine or bilirubinelevation. Captopril with or without bosentan wascytoprotective but did not reverse increased creatinine levels. It is concluded that increased ET-1 inobstructive jaundice may be one of the contributingfactors of renal damage.

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Authors
  1. A. Murat Sarac
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  2. A. Ozdemir Aktan
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  3. Hadi Moini
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  4. Serpil Bilsel
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  5. Eitan Scapa
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Sarac, A.M., Aktan, A.O., Moini, H. et al. Role of Endothelin in Obstructive Jaundice. Dig Dis Sci 44, 356–363 (1999). https://doi.org/10.1023/A:1026614803584

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