Abstract
An oligouridylate with chain-length of more thanthree was condensed on a polyadenylate [poly(A)]template with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDAC) in imidazole buffer. The condensation reactionproceeds via the phosphorimidazolide of theoligouridylate as an intermediate. Pentauridylate[(pU)5] was converted to decauridylate[(pU)10] in 10% yield at 0 °C for 7 daysin the presence of the poly(A) template, while nocoupling product was obtained in the absence of thepoly(A) template. The resulting linkage of the(pU)10 was mainly 2′–5′ linkage.
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Sawai, H., Wada, M. Nonenzymatic Template-Directed Condensation of Short-Chained Oligouridylates on a Poly(A) Template. Orig Life Evol Biosph 30, 503–511 (2000). https://doi.org/10.1023/A:1026583814064
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DOI: https://doi.org/10.1023/A:1026583814064