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Immunization of cross-bred cattle against Hyalomma anatolicum anatolicum by purified antigens

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Abstract

Extracts prepared from unfed larvae of Hyalomma anatolicum anatolicum were purified by immunoaffinity chromatography using anti-gut IgG as ligand. Affinity purified antigen (Aff-GHLAg) was used to immunize cross-bred (Bos taurus × B. indicus) calves of 6–7 months of age. Immunized calves rejected 70.6% larvae, 54.5% nymphs and 61.9% adults. No significant changes in the engorged weight of females was observed; however, significant decrease in the engorgement weight of larvae and nymphs was recorded. There was a significant decrease in the emerging nymphs (p < 0.05) and adults (p < 0.01) of the tick stages fed on immunized animals. SDS-PAGE analysis revealed three antigenic proteins of 100, 59.4 and 37 kDa responsible for induction of resistance in the host.

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Authors and Affiliations

  1. Entomology Laboratory, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243 122, Uttar Pradesh, India

    G. Das, M.H. Khan & J.K. Sharma

  2. Entomology Laboratory, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243 122, Uttar Pradesh, India

    S. Ghosh

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  1. G. Das
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  2. S. Ghosh
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  3. M.H. Khan
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  4. J.K. Sharma
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Das, G., Ghosh, S., Khan, M. et al. Immunization of cross-bred cattle against Hyalomma anatolicum anatolicum by purified antigens. Exp Appl Acarol 24, 645–659 (2000). https://doi.org/10.1023/A:1026581200514

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