Abstract
It is often assumed that all species of arbuscular mycorrhizal fungi (AMF) have the same function because of the ubiquity of the arbuscular mycorrhizal symbiosis and the fact that all AMF occupy the same plant/soil niche. Despite apparent differences in the timing of evolutionary divergence and the morphological characteristics of AMF from the different genera, the majority of studies on these fungi use only species of Glomus. There is increasing evidence, however, that the mechanisms involved in the establishment of a mycorrhiza may differ for species and genera of AMF and influence their subsequent function. The aim of this paper is to highlight the diversity in the form and function of AMF from different genera, knowledge of which is vital in understanding their ecological roles. Potential use of biochemical and molecular approaches to detect AMF in planta and ex planta is also discussed.
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References
Abbott L K 1982 Comparative anatomy of vesicular-arbuscular mycorrhizas formed on subterranean clover. Aust. J. Bot. 30, 485–499.
Abbott L K and Gazey C 1994 An ecological view of the formation of VA mycorrhizas. Plant Soil 159, 69–78.
Abbott L K and Robson A D 1985 Formation of external hyphae in soil by four species of vesicular-arbuscular mycorrhizal fungi. New Phytol. 99, 245–255.
Abbott L K, Robson A D, Jasper D A and Gazey C 1992What is the role of VA Mycorrhizal Hyphae in soil? In Mycorrhizas in Ecosystems. Eds. DJ Read, DH Lewis, AH Fitter and IJ Alexander. pp 37–41. CAB International, University Press Cambridge.
Addy H D, Miller M H and Peterson R L 1997 Infectivity of the propagules associated with extraradical mycelia of two AMfungi following winter freezing. New Phytol. 135, 745–753.
Alexander T, Toth R, Meier R and Weber H C 1989 Dynamics of arbuscule development and degeneration in onion, bean and tomato with particular reference to vesicular-arbuscular mycorrhizae in grasses. Can. J. Bot. 67, 2505–2513.
Allen MF 1996 The ecology of arbuscular mycorrhizas: a look back into the 20th century and a peek into the 21st. Mycol. Res. 100, 769–782.
Ames R N, Ingham E R and Reid C P P 1982 Ultraviolet-induced autofluorescence of arbuscular mycorrhizal root infections: an alternative to cleaning and staining methods for assessing infections. Can. J. Microbiol. 28, 351–355.
Amijee F, Tinker P B and Stribley D P 1989 The development of endomycorrhizal root systems. VII. A detailed study of effects of soil phosphorus on colonisation. New Phytol. 111, 435–446.
Amijee F, Stribley D P and Lane P W 1993 The susceptibility of roots to infection by an arbuscular mycorrhizal fungus in relation to age and phosphorus supply. New Phytol. 125, 581–586.
Bago B, Azcon-Aguilar C and Piche Y 1998 Architecture and developmental dynamics of the external mycelium of the arbuscular mycorrhizal fungus Glomus intraradices grown under monoaxenic conditions. Mycologia 90, 52–62.
BEG 1999 - WWW page URL: wwwbio.ukc.ac.uk/beg
Bentivenga S P and Morton J B 1995 A monograph of the genus Gigaspora, incorporating developmental patterns of morphological characters. Mycologia 87, 719–731.
Boddington C L and Dodd J C 1998 A comparison of the development and metabolic activity of mycorrhizas formed by arbuscular mycorrhizal fungi from different genera on two tropical forage legumes. Mycorrhiza 8, 149–157.
Boddington C L and Dodd J C 1999 Evidence that differences in phosphate metabolism in mycorrhizas formed by species of Glomus and Gigaspora may be related to their life-cycle strategies. New Phytol.
Boddington C L, Bassett E E, Jakobsen I and Dodd J C 1999 Comparison of techniques for the extraction and quantification of extra-radical mycelium of arbuscular mycorrhizal fungi from two soils containing fine particulate matter. Soil Biol. Biochem. 31, 479–482.
Braunberger P G, Abbott L K and Robson A D 1994 The effect of rain in the dry season on the formation of vesicular-arbuscular mycorrhizas in the growing season of annual clover-based pastures. New Phytol. 127, 107–114.
Brundrett M C, Piche Y and Peterson R L 1984 A new method for observing the morphology of vesicular-arbuscular mycorrhizae. Can. J. Bot. 62, 2128–2134.
Brundrett M and Kendrick B 1988 The mycorrhizal status, root anatomy and phenology of plants in a sugar maple forest. Can. J. Bot. 66, 1153–1173.
Brundrett M and Kendrick B 1990 The roots and mycorrhizae of herbaceous woodland plants. II. Structural aspects of morphology. New Phytol. 114, 469–479.
Brundrett M and Abbott L K 1994 Mycorrhizal fungus propagules in the jarrah forest I. Seasonal study of inoculum levels. New Phytol. 127, 539–546.
Brundrett M, Bougher N, Dell B, Grove T and Malajczuk N 1996 Working with Mycorrhizas in Forestry and Agriculture, ACIAR Monograph 32. 373 p.
Claassen V P, Zasoski R J and Tyler B M 1996 A method for direct soil extraction and PCR amplification of endomycorrhizal fungal DNA. Mycorrhiza 6, 447–450.
Clapp J P, Young J P W, Merryweather J and Fitter A H 1995 Diversity of fungal symbionts in arbuscular mycorrhizas from a natural community. New Phytol. 130, 259–265.
Clapp J P, Fitter A H and Young J P W 1999 Ribosomal small subunit sequence variation within spores of an arbuscular mycorrhizal fungus, Scutellospora sp. Molecular Ecology 8, 915–922.
Daniels-Hetrick B A, Bloom J and Feyerherm S M 1985 Root colonization pattern of Glomus epigaeum in nine host species. Mycologia 77, 825–828.
Declerck S, Strullu D G and Plenchette C 1996 In vitro massproduction of the arbuscular mycorrhizal fungus, Glomus versiforme, associated with Ri T-DNA transformed carrot roots. Mycol. Res. 100, 1237–1242.
Di Bonito R, Elliot M L and Des Jardin E A 1995 Detection of an arbuscular mycorrhizal fungus in roots of different plant species with PCR. Appl. Environ Microbiol. 61, 2809–2810.
Dodd J C 1994 Approaches to the study of the extraradical mycelium of arbuscular mycorrhizal fungi. In Impact of Arbuscular Mycorrhizas on Sustainable Agriculture and Natural Ecosystems. Eds. S Gianinazzi and H Schüepp. pp 147-166. Birkhäuser Verlag Basel, Switzerland.
Dodd J C, Arias I, Koomen I and Hayman D S 1990 The management of vesicular-arbuscular mycorrhizal populations in acidinfertile soils of a savanna ecosystem. I. The effect of precropping and VAMF inoculation on plant growth and nutrition in the field. Plant Soil 122, 229–240.
Edwards S G, Fitter A H and Young J P W 1997 Identification of an arbuscular mycorrhizal fungus, Glomus mosseae, within plant roots by competitive polymerase chain reaction. Mycol. Res. 101, 1440–1444.
Ezawa, T, Kuwahara S, Sakamoto K, Yoshida T and Saito M 1999 Specific inhibitor and substrate specificity of alkaline phosphatase expressed in the symbiotic phase of the arbuscular mycorrhizal fungus, Glomus etunicatum. Mycologia 91, 636–641.
Friese C F and Allen M F 1991 The spread of VA mycorrhizal fungi hyphae in the soil: inoculum types and external hyphal structure. Mycologia 83, 409–418.
Gange A C, Bower E, Stagg P G, Aplin D M, Gillam A E and Bracken M 1999 A comparison of visualization techniques for recording arbuscular mycorrhizal colonization. New Phytol. 142, 123–132.
Gazey C, Abbott L K and Robson A D 1992 The rate of development of mycorrhizas affects the onset of sporulation and production of external hyphae by two species of Acaulospora. Mycol. Res. 96, 643–550.
Gazey C, Abbott L K and Robson A D 1993 VA mycorrhizal spores from three species of Acaulospora: germination, longevity and hyphal growth. Mycol. Res. 97, 785–790.
Graham J H, Hodge N C and Morton, J B 1995 Fatty acid methyl ester profiles for characterisation of Glomalean fungi and their endomycorrhizae. Appl. Environ. Microbiol. 61, 58–64.
Graham J H, Duncan L W and Eissenstat D M 1997 Carbohydrate allocation patterns in citrus genotypes as affected by phosphorus nutrition, mycorrhizal colonization and mycorrhizal dependency. New Phytol. 135, 335–343.
Harrison M J 1998 Development of the arbuscular mycorrhizal symbiosis. Current Opinion in Plant Biology 1, 360–365.
Helgason T, Daniell T J, Husband R, Fitter A H and Young J P W 1998 Ploughing up the wood-wide web? Nature 394, 431.
Hepper C M 1985 Influence of age of roots on the pattern of vesicular arbuscular mycorrhizal infection in leek and clover New Phytol. 101, 685–693.
Hepper C M, Sen R and Maskall C S 1986. Identification of vesicular-arbuscular mycorrhizal fungi in roots of leek (Allium porrum L.) and maize (Zea mays L.) on the basis of enzyme mobility during polyacrylamide gel electrophoresis. New Phytol. 102, 529–539.
Hepper C M, Azcon-Aguilar C, Rosendahl S and Sen R 1988 Competition between three species of Glomus used as spatially separated introduced and indigenous mycorrhizal inoculation for leek (Allium porrum L.). New Phytol. 110, 207–215.
INVAM 1999 WWWpage URL: http V ==invam:caf:wvu:edu=
Jakobsen I, Abbott L K and Robson A D 1992a External hyphae of vesicular-arbuscular mycorrhizal fungi associated with Trifolium subterraneum L. 1. Spread of hyphae and phosphorus inflow into roots. New Phytol. 120, 371–380.
Jakobsen I, Abbott L K and Robson A D 1992b External hyphae of vesicular-arbuscular mycorrhizal fungi associated with Trifolium subterraneum L. 2. Hyphal transport of 32P over defined distances. New Phytol. 120, 509–516.
Jasper D A, Robson A D and Abbott L K 1988 Revegetation in an iron-ore mine - nutrient requirements for plant growth and the potential role of vesicular arbuscular (VA) mycorrhizal fungi. Aust. J. Soil Res. 26, 497–507.
Johnson N C, Graham J H and Smith F A 1997 Functioning of mycorrhizal associations along the mutualism-parasitism continuum. New Phytol. 135, 575–585.
Kjoller R and Rosendahl S. 1996 Arbuscular mycorrhizal fungi in roots from a Danish pea field determined by polyacrylamide gel electrophoresis of specific fungal enzymes. Abstract of oral presentation at 1st International Conference onMycorrhizae, San Francisco, CA, USA. July 31- August 3 1996.
Kjoller R and Rosendahl S 2000 - Plant Soil 226, 189–196.
Klironimos J N and Kendrick B 1993 Research on mycorrhizas: trends in the past 40 years as expressed in the 'MYCOLIT' database. New Phytol. 125, 595–600.
Kormanik P P and McGraw A-C 1982 Quantification of vesiculararbuscular mycorrhizae in plant roots. In Methods and Principles of Mycorrhizal Research. Ed. NC Schenck. pp 37–45. St. Paul, MN, USA. American Phytopathological Society.
Lanfranco L, Delpero M and Bonfante P 1999 Intrasporal variability of ribosomal sequences in the endomycorrhizal fungus Gigaspora margarita. Molecular Ecology 8, 37–45.
Larsen J, Olsson P A and Jakobsen I 1998 The use of fatty acid signatures to study mycelial interactions between the arbuscular mycorrhizal fungus Glomus intraradices and the saprotrophic fungus Fusarium culmorum in root-free soil. Mycol. Res. 102, 1491–1496.
Lee P-J and Koske R E Gigaspora gigantea: seasonal abundance and ageing of spores in a sand dune. Mycol. Res. 98, 453- 457.
Lloyd-Macgilp S A, Chambers S M, Dodd J C, Fitter A H, Walker C and Young J P W 1996 Diversity of the internal transcribed spacers within and among isolates of Glomus mosseae and related arbuscular mycorrhizal fungi. New Phytol. 133, 103–112.
Merryweather J and Fitter A 1991 A modified method for elucidating the structure of the fungal partner in a vesicular-arbuscular mycorrhiza. Mycol. Res. 95, 1435–1437.
Merryweather J and Fitter A 1998 The arbuscular mycorrhizal fungi of Hyacinthoides non-scripta. I. Diversity of fungal taxa. New Phytol. 138, 117–129.
Miller R M and Jastrow J D 1992 The role of mycorrhizal fungi in soil conservation. In Proceedings of a Symposium on Mycorrhizae in Sustainable Agriculture. Eds. GJ Bethlenfalvay and RG Linderman. pp 29-44. ASA Special Publication No. 54. Madison, Wisconsin, USA.
Morton J B 1985 Underestimation of most probable numbers of vesicular arbuscular endophytes because of non-staining mycorrhizae. Soil Biol. Biochem. 17, 383–384.
Morton J B and Benny G L 1990 Revised classification of arbuscular mycorrhizal fungi (Zygomycetes): a new order, Glomales, two new suborders, Glomineae and Gigasporineae, and two new families, Acaulosporaceae and Gigasporinaceae, with an emendation of Glomaceae. Mycotaxon 37, 471–491.
Morton J B and Bentivenga S P 1994 Levels of diversity in endomycorrhizal fungi (Glomales, Zygomycetes) and their role in defining taxonomic and non-taxonomic groups. Plant Soil 159, 47–59.
Morton J B 1995 Taxonomic and phylogenetic divergence among five Scutellospora species based on comparative developmental sequences. Mycologia 87, 127–137.
Nicolson T H 1959 Mycorrhiza in the Graminae. I. Vesicular- arbuscular endophytes, with special reference to the external phase. Trans. Brit. Mycol. Soc. 42, 421–438.
Olsson P A, Baath E, Jakobsen I and Soderstrom B 1995 The use of phospholipid and neutral fatty acids to estimate biomass of arbuscular mycorrhizal fungi in soil. Mycol. Res. 99, 623–629.
Pearson J N and Jakobsen I 1993 Symbiotic exchange of carbon and phosphorus between cucumber and three arbuscular mycorrhizal fungi. New Phytol. 124, 481–488.
Pearson J N and Schweiger P 1993 Scutellospora calospora (Nicol. And Gerd.) Walker and Sanders associated with subterranean clover: dynamics of colonisation, sporulation and soluble carbohydrates. New Phytol. 124, 215–219.
Pearson J N, Abbott L K and Robson A D 1993 Mediation of competition between two colonising VA mycorrhizal fungi by the host plant. New Phytol. 123, 93–98.
Phillips J M and Hayman, D S 1970. Improved procedure for clearing roots and staining parasitic and vesicular arbuscular fungi for rapid assessment of infection. Trans. Brit. Mycol. Soc. 55, 158–161.
Read D 1998 Plants on the Web. Nature 396, 22–23.
Riling M C, Wright S F, Allen M F and Field C B 1999 Rise in carbon dioxide changes soil structure. Nature 400, 628.
Rivillas C A 1995 The effects of arbuscular mycorrhizal fungi on two different coffee varieties from Colombia and their biochemical detection in roots. M.Sc. Thesis, University of Kent at Canterbury, UK. 87 p.
Rivillas C and Dodd J C 1996 The effects of arbuscular mycorrhizal fungi on two different coffee varieties from Colombia and their biochemical detection in roots. InMycorrhizas in Integrated Systems from Genes to Plant Development. Proceedings of 4th European Symposium on Mycorrhizas, Granada, Spain 11- 14 July 1994. pp 47–50. EC Brussels.
Rodriguez A and Dodd J C 1998 The use of isozyme markers to screen the intraspecific diversity of isolates of Glomus etunicatum in comparison with other species of Glomus. Abstract poster presented at ICOM2 in Uppsala, Sweden 1998.
Rouhier H and Read D J 1998 The role of mycorrhiza in determining the response of Plantago lanceolata to CO2 enrichment. New Phytol. 139, 367–373.
Rozycka M 1994 Use of biochemical and immunological methods to distinguish arbuscular mycorrhizal fungi. Ph.D. Thesis, University of Kent at Canterbury, UK. 160 p.
Sanders F E, Mosse B and Tinker PB (eds) 1975 Endomycorrhizas. Academic Press, London, UK. 626 p.
Sanders I R, Alt M, Groppe K, Boller T and Wiemken A 1995 Identification of ribosomal DNA polymorphisms among and within spores of the Glomales: application to studies on the genetic diversity of arbuscular mycorrhizal fungal communities. New Phytol. 130, 419–427.
Sanders I R, Clapp J P and Wiemken A 1996 The genetic diversity of arbuscular mycorrhizal fungi in natural ecosystems - a key to understanding the ecology and functioning of the mycorrhizal symbiosis. New Phytol. 133, 123–134.
Sanders I R, Streitwolf-Engel R, Van der Heijden M G A, Boller T and Wiemken A 1998 Increased allocation to external hyphae of arbuscular mycorrhiza fungi under CO2 enrichment. Oecologia 117, 496–503.
Simon L, Lalonde M and Bruns T 1992 Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots. Appl. Environ. Microbiol. 58, 291–295.
Simon L, Bousquet J, Lè vesque R C and Lalonde M 1993a Origin and diversification of endomycorrhizal fungi and coincidence with vascular land plants. Nature 363, 67–69.
Simon L, Vesque R and Lalonde M 1993b Identification of endomycorrhizal fungi colonizing roots by fluorescent single-strand conformation polymorphism-polymerase chain reaction. Appl. Environ. Microbiol. 59, 4211–4215.
Smith F A and Smith S E 1995 Nutrient transfer in vesiculararbuscular mycorrhizas: a new model based on the distribution of ATPases on fungal and plant membranes. Biotropia 8, 1–10.
Smith F A and Smith S E 1996 Mutualism and Parasitism: biodiversity in function and structure in the 'arbuscular' (VA) mycorrhizal symbiosis. Adv. Bot. Res. 22, 1–43.
Smith S E and Read D J (eds) 1997 Mycorrhizal Symbiosis (2nd Edn). Academic Press: London, UK. 605 p.
Staddon P L 1998 Insights into mycorrhizal colonisation at elevated CO2: a simple carbon partitioning model. Plant Soil 205, 171–180.
Stutz J C and Morton J B 1996 Successive pot cultures reveal high species richness of arbuscular endomycorrhizal fungi in arid ecosystems. Can. J. Bot. 74, 1883–1889.
Thomson B D, Robson A D and Abbott K 1990 Mycorrhizas formed by Gigaspora calospora and Glomus fasciculatum on subterranean clover in relation to carbohydrate concentration in roots. New Phytol. 114, 217–255.
Tisdall JM1991 Fungal hyphae and structural stability of soil. Aust. J. Soil Res. 29, 729–743.
Tisdall J M and Oades J M 1979 Organic matter and water-stable aggregates in soils. J Soil Sci. 33, 141–163.
Tisserant B, Brenac V, Requena N, Jeffries P and Dodd J C 1998 The detection of Glomus spp. (arbuscular mycorrhizal fungi) forming mycorrhizas in three plants, at different stages of seedling development, using mycorrhiza-specific isozymes. New Phytol. 138, 225–239.
Van der Heijden M G A, Boller T, Wiemken A and Sanders I R 1998a Different arbuscular mycorrhizal fungal species are potential determinants of plant community structure. Ecology 79, 2082–2091.
Van der Heijden M G A, Klironimos J N, Ursic M, Moutoglis P, Streitwolf-Engel R, Boller T, Wiemken A and Sanders I R 1998b Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature 396, 69–72.
Van Tuinen D, Jacquot E, Zhao B, Gollotte A and Gianinazzi-Pearson V 1998 Characterisation of root colonisation profiles by a microcosm community of arbuscular mycorrhizal fungi using 25 S rDNA-targeted nested PCR. Molecular Ecology 7, 879–887.
Vierheilig H, Coughlan A P, Wyss U and Piche Y 1998 Ink and Vinegar, a simple staining technique for arbuscular-mycorrhizal fungi. Appl. Env. Microbiol. 64, 5004–5007.
Vosatka M and Dodd, J C 1998 The role of different arbuscular mycorrhizal fungi growth of Calamagrostis villosa and Deschampsia flexuosa in experiments with simulated acid rain. Plant Soil, 200, 251–263.
Weickel B 1998 Studies on the isolation, identification and biological characterisation of arbuscular mycorrhizal fungi. Dr. Agr. Thesis Rheinischen Friedrich-Wilhelms-Universitat, Bonn, Germany.
Widden P 1996 The morphology of vesicular-arbuscular mycorrhizae in Clintona borealis and Medeola virginiana. Can. J. Bot. 74, 679–685.
Wilson G W T and Hartnett D C 1997 Effects of mycorrhizae on plant growth and dynamics in experimental tallgrass prairie micrcosms. Am. J. Bot. 84, 478–482.
Wright S F and Upadhyaya A 1996 Extraction of an abundant and unusual protein from soil and comparison with hyphal protein from arbuscular mycorrhizal fungi. Soil Sci. 161, 575–586.
Wright S F and Upadhyaya A 1998 A survey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi. Plant Soil 198, 97–107.
Wright S F, Upadhyaya A and Buyer J S 1998 Comparison of Nlinked oligosaccharides of glomalin from arbuscular mycorrhizal fungi and soils by capillary electrophoresis. Soil Biol. Biochem. 13, 1853–1857.
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Dodd, J.C., Boddington, C.L., Rodriguez, A. et al. Mycelium of Arbuscular Mycorrhizal fungi (AMF) from different genera: form, function and detection. Plant and Soil 226, 131–151 (2000). https://doi.org/10.1023/A:1026574828169
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DOI: https://doi.org/10.1023/A:1026574828169