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The sequence and secondary structure of the 3′-UTR affect 3′-end maturation, RNA accumulation, and translation in tobacco chloroplasts

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Abstract

RNA maturation and modulation of RNA stability play important roles in chloroplast gene expression. In vitro and in vivo studies have shown that both the 5′- and 3′-untranslated regions (UTRs) contain sequence and structural elements that guide these processes, and interact with specific proteins. We have previously characterized the spinach chloroplast petD 3′-UTR in detail by in vitro approaches. This stem-loop forming sequence is a weak terminator but is required for RNA maturation and also exhibits sequence-specific protein binding. To test petD 3′-UTR function in vivo, tobacco chloroplast transformants were generated containing uidA reporter genes flanked by variants of the petD 3′-UTR, including one which does not form an RNA-protein complex in vitro, and one which lacks a stem-loop structure. Analysis of uidA mRNA indicated that a stable secondary structure is required to accumulate a discrete mRNA, and that changes in the 3′-UTR sequence which affect protein binding in vitro can also affect RNA metabolism in vivo. The 3′-UTR also influenced β-glucuronidase protein accumulation, but not in proportion to RNA levels. These results raise the possibility that in tobacco chloroplasts, the 3′-UTR may influence translational yield.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, 14853, USA

    Rita-Ann Monde

  2. Division of Biological Sciences, Cornell University, Ithaca, NY, 14853, USA

    Jessica C. Greene

  3. Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, NY, 14853, USA

    David B. Stern

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  1. Rita-Ann Monde
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  2. Jessica C. Greene
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  3. David B. Stern
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Monde, RA., Greene, J.C. & Stern, D.B. The sequence and secondary structure of the 3′-UTR affect 3′-end maturation, RNA accumulation, and translation in tobacco chloroplasts. Plant Mol Biol 44, 529–542 (2000). https://doi.org/10.1023/A:1026540310934

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