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Rapid Identification of Clavibacter michiganensis Subspecies Sepedonicus based on the Stable Low Molecular Weight RNA (LMW RNA) Profiles

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Abstract

Clavibacter michiganensis subsp. sepedonicus causes potato ring rot disease. The identification process for this bacterium is complex and long. This work demonstrates that the stable low-molecular-weight (LMW) RNA profiles allow their rapid identification. Staircase electrophoresis in polyacrylamide gels was used to analyze the LMW RNA profiles of 54 strains of C. michiganensis subsp. sepedonicus from different geographic origins. The profiles of several strains of other subspecies of C. michiganensis and other pathogens of potatoes were also analyzed. All the strains of C. michiganensis subsp. sepedonicus had the same LMW RNA profile. They had a band in class 2 of tRNA that was absent in the other subspecies of the species C. michiganensis. Also, the LMW RNA of C. michiganensis subsp. sepedonicus was different with respect to the LMW RNA profiles of other pathogens of potato. The results indicate the possible utilization of LMW RNA profiles in identification of the bacteria causing potato ring rot disease.

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References

  • Bidle KD and Fletcher M (1995) Comparison of free-living and particle-associated bacterial communities in the Chesapake Bay by stable low-molecular-weight RNA analysis. Applied and Environmental Microbiology 61: 944–952

    Google Scholar 

  • Council of the European Communities (1993) Directive 93/85/EEC. Official Journal of the European Communities L 259/1

  • Cruz-Sánchez JM, Velázquez E, Mateos PF, Velázquez E and Martínez-Molina E (1997) Enhancement of resolution of low molecular weight RNA profiles by staircase electrophoresis. Electrophoresis 18: 1909–1911

    PubMed  Google Scholar 

  • Haas H, Budowle B and Weiler G (1994) Horizontal polyacrylamide gel electrophoresis for the separation of DNA fragments. Electrophoresis 15: 153–158

    PubMed  Google Scholar 

  • Höfle MG (1988) Identification of bacteria by low molecular weight RNA profiles: a new chemotaxonomic approach. Journal of Microbiological Methods 8: 235–248

    Google Scholar 

  • Li X and de Boer SH (1995) Selection of polymerase chain reaction primers from a RNA intergenic spacer region for specific detection of Clavibacter michiganensis subsp. sepedonicus. Phytopathology 85: 837–842

    Google Scholar 

  • Lows FJ, Bell J, Medina-Mora CM, Smart CD, Opgenorth D, Ishimaru CA, Hausbeck MK, de Bruijn FJ and Fulbright DW (1998) rep-PCR mediated genomic fingerprinting: a rapid and effective method to identify Clavibacter michiganensis. Phytopathology 88: 862–868

    Google Scholar 

  • Pastrik KH and Rainey FA (1999) Identification and differentiation of Clavibacter michiganensis subspecies by polymerase chain reaction-based techniques. Journal of Phytopathology 147: 687–693

    Google Scholar 

  • Pastrik KH (2000) Detection of Clavibacter michiganensis subsp. sepedonicus in potato tubers by multiplex PCR with coamplification of host DNA. European Journal of Plant Pathology 106: 155–165

    Google Scholar 

  • Sprinzl M, Moll J, Meissner F and Hatmann T (1985) Compilation of tRNA sequences. Nucleic Acid Research 13: 1–49

    Google Scholar 

  • Stackebrandt E and Goebel BM (1994) Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. International Journal of Systematic Bacteriology 44: 846–849

    Google Scholar 

  • Velázquez E, Cruz-Sánchez JM, Mateos PF and Martínez-Molina E (1998a) Analysis of stable low-molecular weight RNA profiles of members of the family Rhizobiaceae. Applied and Environmental Microbiology 64: 1555–1559

    PubMed  Google Scholar 

  • Velázquez E, Cervantes E, Igual JM, Peix A, Mateos PF, Benamar S, Moiroud A, Wheeler CT, Dawson J, Labeda D, Rodríguez-Barrueco C and Martínez-Molina E (1998b) Analysis of LMW RNA profiles of Frankia strains by staircase electrophoresis. Systematics and Applied Microbiology 21: 539–545

    Google Scholar 

  • Velázquez E, Calvo O, Cervantes E, Mateos PF, Tamame M and Martínez-Molina E (2000) Staircase electrophoresis profiles of stable low molecular weight RNA as yeast fingerprinting. International Journal of Systematic and Evolutionary Microbiology 50: 917–923

    PubMed  Google Scholar 

  • Vidaver AK (1967) Synthetic and complex media for rapid detection of fluorescence of phytopathogenic pseudomonads: effect of the carbon source. Applied Microbiology 15: 1523–1524

    Google Scholar 

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Authors and Affiliations

  1. Centro Regional de Diagnóstico, Junta de Castilla y León, Salamanca, Spain

    José Luis Palomo & Pablo García-Benavides

  2. Departamento de Microbiología y Genética, Lab 209, Edificio Departamental, Campus Unamuno, Universidad de Salamanca, 37007, Salamanca, Spain;

    Encarna Velázquez

  3. Departamento de Microbiología y Genética, Lab 209, Edificio Departamental, Campus Unamuno, Universidad de Salamanca, 37007, Salamanca, Spain

    Pedro F. Mateos & Eustoquio Martínez-Molina

Authors
  1. José Luis Palomo
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  2. Encarna Velázquez
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  3. Pedro F. Mateos
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  4. Pablo García-Benavides
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  5. Eustoquio Martínez-Molina
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Palomo, J.L., Velázquez, E., Mateos, P.F. et al. Rapid Identification of Clavibacter michiganensis Subspecies Sepedonicus based on the Stable Low Molecular Weight RNA (LMW RNA) Profiles. European Journal of Plant Pathology 106, 789–793 (2000). https://doi.org/10.1023/A:1026539507031

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