Abstract
In sugarcane breeding programs, parents used in crosses are classified as male or female based on relative amounts of viable pollen produced. Usually the ‘female’ parent produces some pollen, albeit at a lower level than the ‘male’ parent. The possibility of selfing and associated problems have long been recognised by breeders. A high level of selfing may seriously bias estimates of breeding value of parents and affect the reliability of family means for identifying superior crosses. However, there is no reliable information reported indicating the level to which selfing is occurring in sugarcane crosses. The level of selfing was evaluated in eight Australian sugarcane crosses using RAPD markers. The crosses were selected as exhibiting variable levels of performance, as judged by measurements of commercial cane sugar (CCS), cane yield (TCH) and sugar yield (TSH) in plots consisting of random progeny clones. Five to eight male-specific RAPD bands were identified for each cross and used to screen twenty-eight to thirty-eight progeny from each cross. Selfed progeny were identified in each cross on the basis of absence of any male-specific RAPD bands after screening with at least 5 such RAPDs. Levels of selfing ranged from 0 to17.6%, with most of the crosses exhibiting zero or low levels of selfing. Although only a limited number of sugarcane crosses have been studied, this survey suggests that unwanted selfing is not a significant problem in the Australian sugarcane breeding program.
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References
Cassalett, C., C.A. Viveros & L.R. Pizza, 1996. Self pollination in sugarcane hybrids, Saccharum sp. Proc Int Soc Sugarcane Technol 22: 312–315.
Divinagracia, N.S., 1980. Emasculation of sugarcane flowers: Steam method. Proc Int Soc Sugar Cane Technol 17: 1287–1295.
Heinz, D.J. & T.L. Tew, 1987. Hybridization Procedures. In: D.J. Heinz (Ed.), Sugarcane Improvement Through Breeding, pp. 313–342. Elsevier Press, Amsterdam.
Hogarth, D.M., 1980. The effect of accidental selfing on the analysis of a diallel cross with sugar cane. Euphytica 29: 737–746.
Machado, J.R., D.I. Walker, J.A. Bressiani & J.A. Goncalves da Silva, 1996. Emasculation of sugarcane tassels using hot water. Proc Int Soc Sugar Cane Technol 22: 346–348.
Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen & R.W. Allard, 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Nat Acad Sciences USA 81: 8014–8018.
Skinner, J.C., 1959. Controlled pollination of sugar cane. Bur Sugar Exp Stn (QLD) Tech Com 1, pp. 1–19.
Stevenson, G.C., 1965. Genetics and Breeding of Sugarcane. Longmans, London, 284 pp.
Tao, Y., J.M. Manners, M.M. Ludlow & R.G. Henzell, 1993. DNA polymorphisms in grain sorghum (Sorghum bicolor (L.) Moench). Theor Appl Genet 86: 679–688.
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McIntyre, C., Jackson, P. Low level of selfing found in a sample of crosses in Australian sugarcane breeding programs. Euphytica 117, 245–249 (2001). https://doi.org/10.1023/A:1026527617892
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DOI: https://doi.org/10.1023/A:1026527617892