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Characterization of three distinct cDNA clones encoding cysteine proteinases from maize (Zea mays L.) callus

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Abstract

In previous work, a 33 kDa cysteine proteinase was found in callus initiated from maize (Zea mays L.) resistant to fall armyworm feeding. A callus cDNA library from the maize inbred Mp708 was screened with oligonucleotides derived from the N-terminal amino acid sequence of the 33 kDa proteinase and several cDNA clones were isolated and sequenced. A cDNA clone encoding the 33 kDa cysteine proteinase, mir1, was identified. Two additional clones, mir2 and mir3, encoding putative cysteine proteinases were also identified. mir2 and mir3 are distinct from mir1 and each other, but show a high degree of homology. All of the mir cDNA clones map to distinct sites on the maize genome. Amino acid sequences encoded by the mir clones are similar to other known cysteine proteinases and are most closely related to the oryzain-α and -β precursors. The ERFNIN motif and a 12 amino acid conserved sequence are present in the propeptide region of the putative proteinases encoded by mir clones. mir2 and mir3 appear to have C-terminal extensions. The phylogenetic tree of nucleotide sequences of mir1, mir2, mir3 and other representative cysteine proteinases from protozoa, plants and animals was constructed.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Mississippi State University, Box 9650, Mississippi State, MS , 39762, USA

    Tibor Pechan, David Steckler, Lei Lin & Dawn S. Luthe

  2. USDA/ARS Host Plant Resistance Laboratory, Mississippi State, MS , 39762, USA

    W. Paul Williams

  3. Scripps Research Institute, La Jolla, CA , 92037, USA

    Binghua Jiang

  4. Department of Medicine, Mount Sinai Medical Center, Box 1243, New York, NY , 10029, USA

    Lijun Ye

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  1. Tibor Pechan
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  2. Binghua Jiang
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  3. David Steckler
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  4. Lijun Ye
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  5. Lei Lin
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  6. Dawn S. Luthe
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  7. W. Paul Williams
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Pechan, T., Jiang, B., Steckler, D. et al. Characterization of three distinct cDNA clones encoding cysteine proteinases from maize (Zea mays L.) callus. Plant Mol Biol 40, 111–119 (1999). https://doi.org/10.1023/A:1026494813936

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  • DOI: https://doi.org/10.1023/A:1026494813936

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