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Synthesis of oligosaccharides by bacterial enzymes

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Abstract

Many human pathogens initiate disease by utilizing their microbial adhesin proteins to attach to glycoconjugates on host cell mucosal surfaces. Soluble oligosaccharides of identical or similar structure to these naturally occurring ligands can both prevent bacterial attachment as well as mediate the release of attached bacteria. Since it has not been possible to isolate large quantities of these compounds, we have developed enzyme-based technologies to synthesize several relevant human oligosaccharides. Using cloned bacterial glycosyltransferases, we can synthesize several hundred grams of these oligosaccharides at a time. The availability of these large quantities will allow these compounds to be tested as anti-adhesive pharmaceutical agents as well as lead to expanded practical applications.

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Authors and Affiliations

  1. Neose Technologies, Inc., Department of Molecular Biology, Horsham, PA, 19044, USA

    Karl F Johnson

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  1. Karl F Johnson
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Johnson, K.F. Synthesis of oligosaccharides by bacterial enzymes. Glycoconj J 16, 141–146 (1999). https://doi.org/10.1023/A:1026440509859

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