Summary
The carbonic anhydrase (CA)-like protein, CA VIII, lacks the typical carbon dioxide hydrase activity of the CA isozymes. However, the high degree of amino acid sequence similarity between the products of the mouse and the human CA VIII genes suggests an important biological function. We have attempted to investigate the function of this gene in mammalian development by conducting an in situ hybridization study on sagittal sections of mouse embryos at gestation days of 9.5–16.5 using a 35S-labelled riboprobe. Results indicate that this gene (called Car8 in mice) is expressed as early as day 9.5 in a variety of organs including liver, branchial arches, neuroepithelium and developing myocardium. Between days 10.5 and 12.5, it showed a widespread distribution of mRNA expression that became more restricted as development progressed. The level of expression of Car8 mRNA was relatively high in the brain, liver, lung, heart, gut, thymus and epithelium covering the head and the oronasal cavity.
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References
Angerer, L.M. & Angerer, R.C. (1981) Detection of poly(A)+ RNA in sea urchin eggs and embryos by quantitative in situ hybridization. Nucleic Acids Res. 9, 2819-840.
Cox, K.H., Deleon, D.V., Angerer, L.M. & Angerer, R.C. (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymetric RNA probes. Dev. Biol. 101, 485-502.
Hayashi, M., Ninomiya, Y., Parsons, J., Hayashi, K., Olsen, B.R. & Trelstad, R.L. (1986) Differential localization of mRNAs of collagen type I and II in chick fibroblasts, chondrocytes and corneal cells by in situ hybridization using cDNA probes. J. Cell. Biol. 102, 2302-9.
Hewett-Emmett, D. & Tashian, R.E. (1996) Functional diversity, conservation and convergence in the evolution of the ·-, ·-and ·-carbonic anhydrase gene families. Mol. Phylogenet. Evol. 5, 50-77.
Kato, K. (1990) Sequence of a novel carbonic anhydrase-related polypeptide and its exclusive presence in the Purkinje cells. FEBS Lett. 271, 137-40.
Kaufman, M.H. (1992) The Atlas of Mouse Development. New York: Academic Press.
Ling, B., Bergenhem, N.C.H., Dodgson, S.J., Forster, R.E. & Tashian, R.E. (1994) Determination of mRNA levels for six carbonic anhydrase isozymes in rat lung. Isozyme Bull. 27, 47.
Melton, D.A., Krieg, P.A., Rebagliati, M.R., Maniatis, T., Zinn, K. & Green, M.R. (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035-56.
Sassoon, D. & Rosenthal, N. (1993) Detection of messenger RNA by in situ hybridization. Methods Enzymol. 225, 384-404.
Skaggs, L.H., Bergenhem, N.C.H., Venta, P.J. & Tashian, R.E. (1993) The deduced amino acid sequence of human carbonic anhydrase-related protein (CARP) is 98% identical to the mouse homologue. Gene 126, 291-2.
Wilkinson, D.G. & Nieto, M.A. (1993) Detection of the messenger RNA by in situ hybridization to tissue sections and whole mounts. Methods Enzymol. 225, 361-73.
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Lakkis, M.M., Bergenhem, N.C., O'Shea, K.S. et al. Expression of the acatalytic carbonic anhydrase VIII gene, Car8, during mouse embryonic development. J Mol Hist 29, 135–141 (1997). https://doi.org/10.1023/A:1026433321974
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DOI: https://doi.org/10.1023/A:1026433321974