Pharmaceutical Research

, Volume 17, Issue 10, pp 1250–1258 | Cite as

Niosomes and Polymeric Chitosan Based Vesicles Bearing Transferrin and Glucose Ligands for Drug Targeting

  • Christine Dufes
  • Andreas G. Schätzlein
  • Laurence Tetley
  • Alexander I. Gray
  • Dave G. Watson
  • Jean-Christophe Olivier
  • William Couet
  • Ijeoma F. Uchegbu


Purpose. To prepare polymeric vesicles and niosomes bearing glucose or transferrin ligands for drug targeting.

Methods. A glucose-palmitoyl glycol chitosan (PGC) conjugate was synthesised and glucose-PGC polymeric vesicles prepared by sonication of glucose-PGC/ cholesterol. N-palmitoylglucosamine (NPG) was synthesised and NPG niosomes also prepared by sonication of NPG/ sorbitan monostearate/ cholesterol/ cholesteryl poly-24-oxyethylene ether. These 2 glucose vesicles were incubated with colloidal concanavalin A gold (Con-A gold), washed and visualised by transmission electron microscopy (TEM). Transferrin was also conjugated to the surface of PGC vesicles and the uptake of these vesicles investigated in the A431 cell line (over expressing the transferrin receptor) by fluorescent activated cell sorter analysis.

Results. TEM imaging confirmed the presence of glucose units on the surface of PGC polymeric vesicles and NPG niosomes. Transferrin was coupled to PGC vesicles at a level of 0.60 ± 0.18 g of transferrin per g polymer. The proportion of FITC-dextran positive A431 cells was 42% (FITC-dextran solution), 74% (plain vesicles) and 90% (transferrin vesicles).

Conclusions. Glucose and transferrin bearing chitosan based vesicles and glucose niosomes have been prepared. Glucose bearing vesicles bind Con-A to their surface. Chitosan based vesicles are taken up by A431 cells and transferrin enhances this uptake.

polymeric vesicles glucose vesicles transferrin vesicles 


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Copyright information

© Plenum Publishing Corporation 2000

Authors and Affiliations

  • Christine Dufes
    • 1
    • 2
  • Andreas G. Schätzlein
    • 3
  • Laurence Tetley
    • 4
  • Alexander I. Gray
    • 1
  • Dave G. Watson
    • 1
  • Jean-Christophe Olivier
    • 2
  • William Couet
    • 2
  • Ijeoma F. Uchegbu
    • 5
  1. 1.Department of Pharmaceutical SciencesUniversity of Strathclyde, Strathclyde Institute for Biomedical SciencesGlasgowUnited Kingdom
  2. 2.Faculté de Médecine et PharmacieLaboratoire de Pharmacie Galénique et BiopharmaciePoitiersFrance
  3. 3.Department of Medical OncologyUniversity of Glasgow, Garscube EstateGlasgowUnited Kingdom
  4. 4.Institute of Biomedical and Life SciencesUniversity of GlasgowGlasgowUnited Kingdom
  5. 5.Department of Pharmaceutical SciencesUniversity of Strathclyde, Strathclyde Institute for Biomedical SciencesGlasgowUnited Kingdom

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