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BAG-1 inhibits p53-induced but not apoptin-induced apoptosis

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Abstract

BAG-1 has been identified as a Bcl-2-binding protein that inhibits apoptosis, either alone or in co-operation with Bcl-2. Here we show that BAG-1 inhibits p53- induced apoptosis in the human tumour cell line Saos-2. In contrast, BAG-1 was unable to inhibit the p53-independent pathway induced by apoptin, an apoptosis-inducing protein derived from chicken anaemia virus. Whereas BAG-1 seemed to co-operate with Bcl-2 to repress p53-induced apoptosis, co-expression of these proteins had no inhibitory effect on apoptin-induced apoptosis. Moreover, Bcl-2, and to some extent also BAG-1, paradoxically enhanced the apoptotic activity of apoptin. These results demonstrate that p53 and apoptin induce apoptosis through independent pathways, which are differentially regulated by BAG-1 and Bcl-2.

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Authors and Affiliations

  1. Laboratory for Molecular Carcinogenesis, Leiden University, PO Box 9503, 2300 RA, Leiden, The Netherlands

    A. A. A. M. Danen-van Oorschot, A. I. den Hollander, A. J. van der Eb & M. H. M. Noteborn

  2. The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, USA

    S. Takayama & J. C. Reed

  3. Leadd BV, PO Box 9503, 2300 RA, Leiden, The Netherlands

    M. H. M. Noteborn

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  1. A. A. A. M. Danen-van Oorschot
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  2. A. I. den Hollander
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  3. S. Takayama
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  4. J. C. Reed
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  5. A. J. van der Eb
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  6. M. H. M. Noteborn
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Danen-van Oorschot, A.A.A.M., den Hollander, A.I., Takayama, S. et al. BAG-1 inhibits p53-induced but not apoptin-induced apoptosis. Apoptosis 2, 395–402 (1997). https://doi.org/10.1023/A:1026409808732

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